FastQCFastQC Report
Wed 8 May 2024
EGAF00003947752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9579227
Total Bases213.1 Mbp
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8550748.926336122946037No Hit
CAACGGAATCCCAAAAGCAGCTG6484616.769450186325056No Hit
CCCCCACTGCTAAATTTGACTG3431583.5823141053030687No Hit
AAGCTGCCAGTTGAAGAACTGT3219613.361033202365911No Hit
AACATTCAACGCTGTCGGTGAGT2971683.1022127359545815No Hit
TAGCAGCACGTAAATATTGGCG2852532.9778290043653834No Hit
TTCAAGTAATCCAGGATAGGCT2763222.884596011765876No Hit
AACATTCAACGCTGTCGGTGAG2229002.326910094102583No Hit
TAGCTTATCAGACTGATGTTGAC1892941.97608846726359No Hit
TAGCTTATCAGACTGATGTTGACT1281901.3382081873620908No Hit
CGCCTGTCTGAGCGTCGCT1189471.2417181469861818No Hit
TATTGCACTTGTCCCGGCCTGTAA1071131.1181799951081648No Hit
TGTAAACATCCTTGACTGGAAGCT1065881.1126993858690268No Hit
TGAGGTAGTAGGTTGTATAGTT966821.0092881189682634No Hit
TAGCTTATCAGACTGATGTTGA941250.9825949421597379No Hit
TATTGCACTTGTCCCGGCCTGTAT924030.9646185438553654No Hit
AGCAGCATTGTACAGGGCTATGA766940.8006282761646635No Hit
CATAAAGTAGAAAGCACTACT738950.7714087994782878No Hit
TATTGCACTTGTCCCGGCCTGT723940.7557394766822No Hit
GCCTGTCTGAGCGTCGCT587520.6133271505101613No Hit
TATTGCACTTGTCCCGGCCTGTA570850.5959249112689364No Hit
ACTGGACTTGGAGTCAGAAGGC526600.5497312048247734No Hit
TGTAAACATCCCCGACTGGAAGCT518160.5409204730193783No Hit
TGAGGTAGTAGATTGTATAGTT512660.5351788823879003No Hit
TTCAAGTAATTCAGGATAGGTT465040.4854671467749956No Hit
TACGCCTGTCTGAGCGTCGCT430480.4493890790979272No Hit
CTACGCCTGTCTGAGCGTCGCT353780.3693199879280447No Hit
CAACGGAATCCCAAAAGCAGCTGT337400.3522204871019342No Hit
CCCATAAAGTAGAAAGCACTAC334350.3490365141153874No Hit
TGTCAGTTTGTCAAATACCCCAA331570.34613440103256765No Hit
AACCGGGCGGAAACACCA321500.33562207054911636No Hit
CATTGCACTTGTCTCGGTCTGA307940.32146643982859996No Hit
CAACACCAGTCGATGGGCTGT290520.3032812564103554No Hit
TGTAAACATCCTACACTCTCAGCT277550.2897415417757612No Hit
AACATTCAACGCTGTCGGTGAGTT268110.2798868844010065No Hit
ACGCCTGTCTGAGCGTCGCT264950.2765880796018301No Hit
CCCATAAAGTAGAAAGCACT262550.274082658235367No Hit
GCGTTCGCGCTTTCCCCTG252310.26339286040512455No Hit
TGAGGTAGTAGTTTGTACAGTT246490.2573172135914516No Hit
CACCCGGCTGTGTGCACATGTGC234940.24525987326534804No Hit
CAACGGAATCCCAAAAGCAGCT226850.23681451540922874No Hit
TAGCAGCACGTAAATATTGGC222470.2322421214154336No Hit
TGTCAGTTTGTCAAATACCCCA214560.22398467016179904No Hit
TAGCTTATCAGACTGATGTTGACA211090.22036224843612118No Hit
TTCTCACTACTGCACTTGACTAG203390.21232402155205218No Hit
TTCACAGTGGCTAAGTTCCG190230.19858596105927964No Hit
CCCCCACTGCTAAATTTGACTGGCTT189610.19793872720627667No Hit
AACGGAATCCCAAAAGCAGCTG186680.194880025288053No Hit
ACCCCCCACTGCTAAATTTGACTG184660.19277129563794657No Hit
ACCGGGTGCTGTAGGCTT175300.1830001523087406No Hit
CTTCTCACTACTGCACTTGACTAG174450.1821128155747849No Hit
TCGTACGACTCTTAGCGG167870.17524378532839863No Hit
TGTAAACATCCTACACTCAGCT164220.17143345700023602No Hit
TTATAAAGCAATGAGACTGATT163900.17109940081804095No Hit
TCCTGTCTGAGCGTCGCT163830.17102632602818577No Hit
TTCAAGTAATCCAGGATAGGCTT159840.1668610630064409No Hit
TGAGGTAGTAGTTTGTGCTGTT157640.16456442675384975No Hit
AGCAGCATTGTACAGGGCTATGT155050.16186065952920836No Hit
CTCCCACTGCTTCACTTGACTAG150500.15711079818862211No Hit
ACTGGACTTGGAGTCAGAAGGCA150290.15689157381905658No Hit
CAAAGAATTCTCCTTTTGGGCT148440.1549603115157413No Hit
ACTGGACTTGGAGTCAGAAGG146630.1530708062352004No Hit
TCGTTTCCCGGCCAATGCACCA143350.14964673036770088No Hit
CCTCGACACAAGGGTTTGT138510.1445941306120003No Hit
TAGCAGCACGTAAATATTGGCGT137120.14314307407059046No Hit
TGTAAACATCCCCGACTGGAAGC137070.14309087779212246No Hit
ACCACAGGGTAGAACCACGGAC135920.14189036338735891No Hit
TATTGCACTTGTCCCGGCCTGTT134940.1408673163293865No Hit
CCCCCCACTGCTAAATTTGACTGG133030.13887341849190962No Hit
ACTGGACTTGGAGTCAGAAGGCT127850.13346588404262683No Hit
TTCAAGTAATCCAGGATAGGC125320.130824752352147No Hit
AGCAGCATTGTACAGGGCTATG121360.12669080709748293No Hit
CAAAGAATTCTCCTTTTGGGCTT119960.12522931130037945No Hit
TTCACAGTGGCTAAGTTCCGC119790.12505184395358834No Hit
GGAATACCGGGTGCTGTAGGCTTT119510.12475954479416763No Hit
AGAATTACTCTGAGACCT119460.12470734851569965No Hit
TATTGCACTTGTCCCGGCCTGTAG119060.1242897782879558No Hit
ATCCCGGACGAGCCCCCA118910.12413318945255186No Hit
TGAGGTAGTAGGTTGTGTGGTT117140.12228544119478535No Hit
ATCACATTGCCAGGGATTTCC116070.12116844083557056No Hit
ACCGGGTGCTGTAGGCTTT115830.12091789869892425No Hit
ATCACATTGCCAGGGATTTCCA113840.1188404868158986No Hit
CCAAGTCCTTCTGATCGAGGCCC113390.11837072030968679No Hit
TTCACAGTGGCTAAGTTCTG113020.11798446784902372No Hit
CAAAGTGCTGTTCGTGCAGGTAG112710.11766085092252225No Hit
CCCCCCACTGCTAAATTTGACTGGCT109050.11384008333866606No Hit
CACTAGATTGTGAGCTCCTGGA108220.11297362511609758No Hit
TTCACAGTGGCTAAGTTCTGC104280.10886055837282069No Hit
CTCGACACAAGGGTTTGT103050.10757652992250835No Hit
CACTAGATTGTGAGCTCCTGGAA102020.10650128658606796No Hit
ACCGGGTGCTGTAGGCTTA100670.10509198706743247No Hit
AGCTCGGTCTGAGGCCCCTCAGT99680.10405850075376646No Hit
TACCTGGTTGATCCTGCC98970.10331731359952112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)