FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8280061
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5169626.243456418980489No Hit
AAGCTGCCAGTTGAAGAACTGT3412224.121008287257787No Hit
CCCCCCACTGCTAAATTTGACTG2989533.6105168790423163No Hit
TAGCAGCACGTAAATATTGGCG2875833.473199050103616No Hit
TTCAAGTAATCCAGGATAGGCT2863343.458114620170069No Hit
AACATTCAACGCTGTCGGTGAGT1803352.1779428919666173No Hit
GCCTGTCTGAGCGTCGCT1760422.126095447847546No Hit
TGAGGTAGTAGGTTGTATAGTT1237641.4947232876666006No Hit
TAGCTTATCAGACTGATGTTGAC1199581.4487574427289847No Hit
CCCCCACTGCTAAATTTGACTG1075501.2989034742618442No Hit
AACCGGGCGGAAACACCA980281.1839043214778249No Hit
AACATTCAACGCTGTCGGTGAG969051.1703416194639147No Hit
TAGCTTATCAGACTGATGTTGA959641.1589769688894804No Hit
TATTGCACTTGTCCCGGCCTGT932891.126670443611466No Hit
TAGCTTATCAGACTGATGTTGACT876451.0585066945762838No Hit
TATTGCACTTGTCCCGGCCTGTAA869411.0500043417554532No Hit
CATAAAGTAGAAAGCACTACT844911.0204151877625056No Hit
CGCCTGTCTGAGCGTCGCT836961.0108138092219368No Hit
TGAGGTAGTAGATTGTATAGTT768400.9280124868645292No Hit
TATTGCACTTGTCCCGGCCTGTAT748330.9037735350017349No Hit
TGTAAACATCCTTGACTGGAAGCT673370.8132428009890265No Hit
TTCAAGTAATTCAGGATAGGTT606980.7330622322709941No Hit
AGCAGCATTGTACAGGGCTATGA506920.6122177119226537No Hit
ACTGGACTTGGAGTCAGAAGGC427020.5157208382885102No Hit
TATTGCACTTGTCCCGGCCTGTA419360.5064696987135723No Hit
CAACGGAATCCCAAAAGCAGCTGT389780.47074532421922977No Hit
CCCATAAAGTAGAAAGCACTAC355200.4289823468691837No Hit
CTACGCCTGTCTGAGCGTCGCT349000.42149447932811124No Hit
TCGTACGACTCTTAGCGG344320.41584234705517265No Hit
TGTAAACATCCCCGACTGGAAGCT341880.4128955088615893No Hit
TTCTCACTACTGCACTTGACTAG305120.36849970066645643No Hit
CATTGCACTTGTCTCGGTCTGA300410.36281133677638366No Hit
TGAGGTAGTAGTTTGTACAGTT298680.3607219801883102No Hit
TACGCCTGTCTGAGCGTCGCT293540.35451429645264687No Hit
CCCATAAAGTAGAAAGCACT280680.33898300990777724No Hit
ACCGGGTGCTGTAGGCTT273800.33067389237832906No Hit
CACCCGGCTGTGTGCACATGTGC264750.31974402120950557No Hit
TGTAAACATCCTACACTCTCAGCT257010.3103962639888764No Hit
ACGCCTGTCTGAGCGTCGCT247540.29895915018017377No Hit
CTCCCACTGCTTCACTTGACTAG234030.28264284526406264No Hit
CTTCTCACTACTGCACTTGACTAG221830.2679086542961459No Hit
GCGTTCGCGCTTTCCCCTG216510.26148358085767726No Hit
CCCCCACTGCTAAATTTGACTGGCTT214540.25910437133253No Hit
TGAGGTAGTAGTTTGTGCTGTT210250.25392325008233635No Hit
ACCGGGTGCTGTAGGCTTT193650.2338750886014004No Hit
ATCCCGGACGAGCCCCCA187690.2266770739974017No Hit
CAACACCAGTCGATGGGCTGT184210.222474206409832No Hit
ACCGGGTGCTGTAGGCTTA175120.21149602641816287No Hit
GGAATACCGGGTGCTGTAGGCTTT169040.2041530853456273No Hit
CCCCCCACTGCTAAATTTGACTGGCT163840.19787293837569553No Hit
AACATTCAACGCTGTCGGTGAGTT157150.18979328775476412No Hit
TGTCAGTTTGTCAAATACCCCAA152700.18441893121318792No Hit
TAGCAGCACGTAAATATTGGCGT150340.1815687106652958No Hit
TTCAAGTAATCCAGGATAGGC149990.18114600846539658No Hit
TTCACAGTGGCTAAGTTCCG148920.179853747454276No Hit
AGCTCGGTCTGAGGCCCCTCAGT147970.17870641291169231No Hit
ATCCCACCGCTGCCACCA147660.1783320195346387No Hit
TAGCAGCACGTAAATATTGGC147500.1781387842432562No Hit
TTATAAAGCAATGAGACTGATT146100.17644797544365917No Hit
TTCAAGTAATCCAGGATAGGCTT142160.17168955639336472No Hit
TGTAAACATCCTACACTCAGCT141450.17083207478785484No Hit
GGAATACCGGGTGCTGTAGGCTT141310.17066299390789513No Hit
GGGCTACGCCTGTCTGAGCGTCGCT139040.1679214682114057No Hit
TATTGCACTTGTCCCGGCCTGTT135690.1638756042980843No Hit
TGATGTAGTAGGTTGTATAGTT130090.15711236909969625No Hit
TGAGGTAGTAGGTTGTGTGGTT128710.15544571471152205No Hit
TTCACAGTGGCTAAGTTCCGC127040.15342882135771704No Hit
AACGGAATCCCAAAAGCAGCTG126990.15336843532916003No Hit
AGCAGCATTGTACAGGGCTATGT124890.1508322221297645No Hit
TGAGGTAGTAGGTTGTATAGT122720.14821146849038916No Hit
TGTCAGTTTGTCAAATACCCCA121070.14621872954800694No Hit
CCCCCCACTGCTAAATTTGACTGG119490.14431053104560462No Hit
TTCACAGTGGCTAAGTTCTGC119200.1439602920799738No Hit
TAGCTTATCAGACTGATGTTGACA118800.14347720385151752No Hit
CAACGGAATCCCAAAAGCAGCT117410.14179847225763192No Hit
TACCTGGTTGATCCTGCC115990.1400835090466121No Hit
CTCCCACTGCTTCACTTGACTAGCCT110860.1338879025166602No Hit
CACTAGATTGTGAGCTCCTGGA109390.13211255327708332No Hit
CAAAGAATTCTCCTTTTGGGCT104890.1266778107069501No Hit
AGCAGCATTGTACAGGGCTATG102310.12356189163340704No Hit
ACTGGACTTGGAGTCAGAAGG101840.1229942629649709No Hit
TGTAAACATCCCCGACTGGAAGC101660.12277687326216558No Hit
CAAAGAATTCTCCTTTTGGGCTT97630.11790975936046848No Hit
CAAAGTGCTGTTCGTGCAGGTAG96730.11682281084644183No Hit
ACCACAGGGTAGAACCACGGAC95830.11573586233241519No Hit
CGCGACCTCAGATCAGACGTGGCG95290.1150836932239992No Hit
AGAGGTAGTAGGTTGCATAGTT93820.11330834398442233No Hit
TAGCAGCACATAATGGTTTGT91280.11024073373372492No Hit
CCTCGACACAAGGGTTTGT90950.10984218594524847No Hit
TATTGCACTTGTCCCGGCCTGTAG89800.10845330728843665No Hit
GCTTGACTAGCTTGCTGTTT88980.10746297642010125No Hit
CAAAGTGCTGTTCGTGCAGGT88940.10741466759725561No Hit
ACCCCCCACTGCTAAATTTGACTG87570.10576009041479283No Hit
ACTGGACTTGGAGTCAGAAGGCA87190.10530115659775938No Hit
CTCGACACAAGGGTTTGT86740.10475768234074605No Hit
CGCGACCTCAGATCAGACGTGG84350.10187123017571971No Hit
TGATGTAGTAGATTGTATAGTT83970.10141229635868626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)