FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6541796
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG107686016.461228690102843No Hit
CCCCCCACTGCTAAATTTGACTG3661835.597591242527281No Hit
TCGTACGACTCTTAGCGG2236643.418999919899673No Hit
TTCAAGTAATCCAGGATAGGCT1741282.66177667417327No Hit
CCCCCACTGCTAAATTTGACTG1564742.3919119458937574No Hit
TGAGGTAGTAGATTGTATAGTT1342992.052937755931246No Hit
AACATTCAACGCTGTCGGTGAG1180701.804856036476833No Hit
TGAGAACTGAATTCCATGGGTT1165791.7820641304008868No Hit
TAGCAGCACGTAAATATTGGCG1027011.5699205539273926No Hit
AAGCTGCCAGTTGAAGAACTGT919891.406173472850575No Hit
TATTGCACTTGTCCCGGCCTGT881881.348070162994994No Hit
TGAGGTAGTAGGTTGTATAGTT839191.2828128544515909No Hit
TATTGCACTTGTCCCGGCCTGTAA829081.2673583829272572No Hit
TAGCTTATCAGACTGATGTTGAC714281.0918714065678599No Hit
TATTGCACTTGTCCCGGCCTGTAT632890.9674560319520816No Hit
CACCCGGCTGTGTGCACATGTGC595150.909765452790029No Hit
TAGCTTATCAGACTGATGTTGA515790.7884532015367034No Hit
CAACGGAATCCCAAAAGCAGCTGT444160.6789572771758704No Hit
TAGCTTATCAGACTGATGTTGACT424210.6484610648207312No Hit
TGAGGTAGTAGTTTGTACAGTT423460.6473145906720417No Hit
TATTGCACTTGTCCCGGCCTGTA409730.6263264705900337No Hit
TTCAAGTAATTCAGGATAGGTT377810.577532530821811No Hit
GCCTGTCTGAGCGTCGCT365530.5587609274272691No Hit
AACATTCAACGCTGTCGGTGAGT336960.5150879055231927No Hit
AGCAGCATTGTACAGGGCTATGA307740.470421272690252No Hit
TGTAAACATCCTTGACTGGAAGCT284020.43416211694770057No Hit
AACGGAATCCCAAAAGCAGCTG279570.42735970366547654No Hit
ACCGGGTGCTGTAGGCTT278080.4250820416900802No Hit
ACTGGACTTGGAGTCAGAAGGC277800.42465402467456953No Hit
CCCCCACTGCTAAATTTGACTGGCTT264720.40465951552142565No Hit
CTCCCACTGCTTCACTTGACTAG262690.4015563921589729No Hit
TGAGAACTGAATTCCATAGGCTGT260960.39891185845599586No Hit
TTAATGCTAATCGTGATAGGGGTT260590.39834626454264244No Hit
CATAAAGTAGAAAGCACTACT258570.39525842750217216No Hit
CATTGCACTTGTCTCGGTCTGA255200.39010693699406096No Hit
TTCTCACTACTGCACTTGACTAG239110.3655112449241768No Hit
CAACGGAATCCCAAAAGCAGCT227790.3482071284399575No Hit
ACCGGGTGCTGTAGGCTTT213820.3268521366303688No Hit
TCGTTTCCCGGCCAATGCACCA209100.31963699265461654No Hit
CCCATAAAGTAGAAAGCACT198640.30364749986089445No Hit
CCCATAAAGTAGAAAGCACTAC179660.2746340607380603No Hit
TGTAAACATCCCCGACTGGAAGCT179500.27438947958633986No Hit
ACCGGGTGCTGTAGGCTTA163720.250267663497914No Hit
GGAATACCGGGTGCTGTAGGCTTT162330.24814286474234296No Hit
TGAGGTAGTAGATTGTATAGT152400.23296354701369473No Hit
GGAATACCGGGTGCTGTAGGCTT147750.22585540729182016No Hit
CTCCCACTGCTTCACTTGACTAGCCT138840.2122352944053896No Hit
CTTCTCACTACTGCACTTGACTAG137590.21032450415757387No Hit
TGAGAACTGAATTCCATGGGTTG130410.19934892497412027No Hit
ACCCCCCACTGCTAAATTTGACTG130080.19884447634869692No Hit
TTCACAGTGGCTAAGTTCCGC127220.1944725882616945No Hit
CAACACCAGTCGATGGGCTGT117370.17941556110890647No Hit
AGCTCGGTCTGAGGCCCCTCAGT116330.1778257836227238No Hit
CACTAGATTGTGAGCTCCTGGA115520.1765875915421392No Hit
TGATGTAGTAGATTGTATAGTT108170.16535214488498265No Hit
TAGCAGCACGTAAATATTGGC105840.16179043186305414No Hit
CCCCCCACTGCTAAATTTGACTGG104790.1601853680548889No Hit
CCCCCCACTGCTAAATTTGACTGGCT103990.15896246229628683No Hit
TATTGCACTTGTCCCGGCCTGTT101290.15483515536100484No Hit
TCGTTTCCCGGCCAACGCACCA94850.14499076400425814No Hit
TGAGGTAGTAGTTTGTGCTGTT94140.14390543514349882No Hit
TACCACCCTGAACGCGCCCGA93720.14326340962023273No Hit
TCCCACTGCTTCACTTGACTAG92390.14123032879655678No Hit
TTCACAGTGGCTAAGTTCTGC92250.14101632028880143No Hit
CACTAGATTGTGAGCTCCTGGAA91950.14055773062932564No Hit
TCCTGTACTGAGCTGCCCCGAG91200.1394112564806362No Hit
CCCATAAAGTAGAAAGCACTA88620.13546738540914452No Hit
TATTGCACTTGTCCCGGCCTGTAG87560.13384703527899677No Hit
TGATAACTGAATTCCATGGGTT86980.13296042860401028No Hit
TCCTGTACTGAGCTGCCCCGAGT86970.13294514228202775No Hit
TAGCTTATCAGACTGATGTTGACA86740.13259355687642965No Hit
TGAGGTAGTAAGTTGTATTGTT83370.12744206636831842No Hit
TGGCTCAGTTCAGCAGGAAC80840.12357462690673938No Hit
ACTGGACTTGGAGTCAGAAGGCA80580.12317718253519369No Hit
CTCGTACGACTCTTAGCGG79590.12166383665892364No Hit
ACTGGACTTGGAGTCAGAAGGCT79260.12115938803350028No Hit
TGTAAACATCCCCGACTGGA78220.11956961054731757No Hit
CCACCCTGAACGCGCCCGA76130.11637476925296968No Hit
TGTCAGTTTGTCAAATACCCCA75700.11571745740772106No Hit
TTCAAGTAATCCAGGATAGGC74870.11444869268317141No Hit
TCGATTCCCGGCCAACGCACCA73220.11192644955605464No Hit
GAATACCGGGTGCTGTAGGCTTT73140.11180415898019443No Hit
GTCAGGATGGCCGAGCGGTCTAAGG72030.11010737724013406No Hit
CAAAGTGCTGTTCGTGCAGGTAG70560.10786028790870275No Hit
TGATGTAGTAGGTTGTATAGTT69550.10631636938846763No Hit
TGTAAACATCCTACACTCTCAGCT69110.1056437712212365No Hit
AATGCACCCGGGCAAGGATTCT68800.1051698952397782No Hit
TTCACAGTGGCTAAGTTCTG67000.10241835728292353No Hit
TTCAAGTAATCCAGGATAGGCTT66860.10220434877516818No Hit
TCCTGTACTGAGCTGCCCCGAGA65730.10047699439114273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)