FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6961546
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG85535112.286796639711927No Hit
CAACGGAATCCCAAAAGCAGCTG84071712.076584712648598No Hit
CCCCCACTGCTAAATTTGACTG2433673.4958757724218157No Hit
TTCAAGTAATCCAGGATAGGCT2215273.1821523552383337No Hit
CCCCCACTGCTAAATTTGACTGGCTT649500.9329824151129649No Hit
AACATTCAACGCTGTCGGTGAGT619280.8895725173689867No Hit
CAACGGAATCCCAAAAGCAGCTGT593680.8527990765269668No Hit
GCCTGTCTGAGCGTCGCT586860.8430024020526475No Hit
ACTGGACTTGGAGTCAGAAGGC569770.8184532573655334No Hit
CTCCCACTGCTTCACTTGACTAG547830.7869372693938961No Hit
TAGCAGCACGTAAATATTGGCG514050.7384135650328246No Hit
CCCCCCACTGCTAAATTTGACTGGCT493670.7091384586124978No Hit
CGCCTGTCTGAGCGTCGCT486830.6993130548875207No Hit
CACCCGGCTGTGTGCACATGTGC429380.6167882823729096No Hit
CCCATAAAGTAGAAAGCACTAC399130.5733352907529448No Hit
ACCGGGTGCTGTAGGCTTA375480.5393629518500632No Hit
CATAAAGTAGAAAGCACTACT365220.5246248462625974No Hit
AAGCTGCCAGTTGAAGAACTGT360330.5176005444767585No Hit
AACATTCAACGCTGTCGGTGAG357550.5136071786353203No Hit
AGCAGCATTGTACAGGGCTATGA343350.4932094106682625No Hit
CTACGCCTGTCTGAGCGTCGCT341370.4903652148531375No Hit
AACCGGGCGGAAACACCA326030.4683298795985834No Hit
CCCCCCACTGCTAAATTTGACTGG291130.41819733720067354No Hit
CCCATAAAGTAGAAAGCACT282250.4054415499085979No Hit
ACCGGGTGCTGTAGGCTT275510.3957597924369099No Hit
AGCTCGGTCTGAGGCCCCTCAGT259470.37271893340933177No Hit
ACCGGGTGCTGTAGGCTTT257280.36957308046229964No Hit
TACGCCTGTCTGAGCGTCGCT250010.3591299978481791No Hit
CAACGGAATCCCAAAAGCAGCT243390.3496206158804381No Hit
CAACACCAGTCGATGGGCTGT235740.33863167750381884No Hit
CATTGCACTTGTCTCGGTCTGA227870.32732671736996355No Hit
CACTAGATTGTGAGCTCCTGGAA225420.32380738416437954No Hit
CACTAGATTGTGAGCTCCTGGA222980.3203024155841246No Hit
GACCCGCGGGCGCTCTCCAGTCCT220100.31616540348939737No Hit
TCGTACGACTCTTAGCGG217400.31228695465059053No Hit
CTTCTCACTACTGCACTTGACTAG212350.30503281885948896No Hit
TTCAAGTAATTCAGGATAGGTT189400.27206600372963136No Hit
CAAAGAATTCTCCTTTTGGGCT187620.2695091004210846No Hit
TGAGGTAGTAGGTTGTATAGTT175760.2524726547809926No Hit
ACTGGACTTGGAGTCAGAAGGCT168060.24141189327772883No Hit
CCCCCACTGCTAAATTTGACTGGCT166930.23978869061556154No Hit
ATCCCGGACGAGCCCCCA155040.22270915109948278No Hit
TGTAAACATCCTACACTCTCAGCT152320.2188019730100182No Hit
TAGCTTATCAGACTGATGTTGA147350.2116627542215479No Hit
TAGCTTATCAGACTGATGTTGAC144370.2073820958735315No Hit
CCCCCCACTGCTAAATTTGACTGT142890.20525613132485226No Hit
TTCTCACTACTGCACTTGACTAG135570.1947412255840872No Hit
CAAAGAATTCTCCTTTTGGGCTT132780.19073349511732018No Hit
TCCTGTCTGAGCGTCGCT132230.18994344072422994No Hit
CGCGACCTCAGATCAGACGTGGCG130800.18788929930219522No Hit
ACGCCTGTCTGAGCGTCGCT130510.18747272516765673No Hit
AACGGAATCCCAAAAGCAGCTG130480.18742963129166998No Hit
CGCGACCTCAGATCAGACGTGGC128460.18452797697522935No Hit
TTCAAGTAATCCAGGATAGGCTT127460.18309151444233795No Hit
ACCCCCCACTGCTAAATTTGACTG127240.18277549268510185No Hit
CGCGACCTCAGATCAGACGT124720.1791556071022155No Hit
TTCACAGTGGCTAAGTTCCG123450.17733129968544342No Hit
ACTGGACTTGGAGTCAGAAGG118270.16989042376506597No Hit
CCCCCCACTGCTAAATTTGACTGTA117920.169387661878554No Hit
ATAGGCGATAGAAATTGAAACCT111460.16010811391607555No Hit
ACTGGACTTGGAGTCAGAAGGCA107580.1545346392884569No Hit
TGAGGTAGTAGATTGTATAGTT103630.14886061228353586No Hit
CCCCCCACTGCTAAATTTGACTGGC103090.14808492251577451No Hit
AGCTACATCTGGCTACTGGGTCTC102120.14669155385886984No Hit
GGAATACCGGGTGCTGTAGGCTTT102030.14656227223090962No Hit
AGCTACATTGTCTGCTGGGTTT100810.14480978794078211No Hit
CCCATAAAGTAGAAAGCACTA98200.14106062072993555No Hit
GACCCGCGGGCGCTCTCCAGTCCTT93500.13430924682534598No Hit
TATTGCACTTGTCCCGGCCTGTAA92880.1334186400549533No Hit
CAACGGAATCCCAAAAGCAGCTGA91900.13201090677271973No Hit
CTCCCACTGCTTCACTTGACTAGCCT89290.12826173956187317No Hit
TCGTTTCCCGGGCGGCGCACCA83130.11941313035926215No Hit
TTAATGCTAATCGTGATAGGGGTT81520.11710042568130699No Hit
CCCCCACAACCGCGCTTGACTAG80120.11508937813525903No Hit
ACCACAGGGTAGAACCACGGAC78950.11340871697177608No Hit
CCCCCACTGCTAAATTTGACTGGCTA78530.1128054027079617No Hit
TAGCTTATCAGACTGATGTTGACT74960.10767723146553941No Hit
AGCAGCATTGTACAGGGCTATGT72850.10464629552113855No Hit
AACATTCAACGCTGTCGGTGAGTT72650.10435900301456026No Hit
CCCCCCACTGCTAAATTTGACTGA72440.10405734588265307No Hit
AGCTACATCTGGCTACTGGGTCT71750.10306618673495802No Hit
CCCCCACTGCTAAATTTGACTGG70590.10139989019680398No Hit
GGAATACCGGGTGCTGTAGGCTT70130.10073911743167395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)