FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10508911
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8735088.31206963309519No Hit
CCCCCCACTGCTAAATTTGACTG7715357.341721706464162No Hit
TTCAAGTAATCCAGGATAGGCT3778393.595415357499935No Hit
AAGCTGCCAGTTGAAGAACTGT3079502.930370235317437No Hit
TAGCAGCACGTAAATATTGGCG2672022.542623112899139No Hit
AACATTCAACGCTGTCGGTGAGT2580512.4555446325504136No Hit
CCCCCACTGCTAAATTTGACTG2573892.449245216749861No Hit
TAGCTTATCAGACTGATGTTGAC1780151.6939433591168487No Hit
AACATTCAACGCTGTCGGTGAG1604471.526770947056265No Hit
TAGCTTATCAGACTGATGTTGACT1574511.4982618084785377No Hit
TGAGGTAGTAGGTTGTATAGTT1559821.4842831954709674No Hit
TATTGCACTTGTCCCGGCCTGTAA1229681.170130758553384No Hit
CCCCCACTGCTAAATTTGACTGGCTT1184901.1275193024281964No Hit
TAGCTTATCAGACTGATGTTGA1064201.0126643950072467No Hit
TATTGCACTTGTCCCGGCCTGTAT930890.8858101472169666No Hit
TGAGGTAGTAGATTGTATAGTT901440.857786311064962No Hit
TGTAAACATCCTTGACTGGAAGCT878420.8358810917705936No Hit
TCCACCACGTTCCCGTGG822260.7824407305381118No Hit
CCCATAAAGTAGAAAGCACTAC772240.7348430298819735No Hit
AGCAGCATTGTACAGGGCTATGA693950.6603443496666781No Hit
CATAAAGTAGAAAGCACTACT674940.6422549396412245No Hit
TAGCAGCACATCATGGTTTACA614210.5844658880449173No Hit
TATTGCACTTGTCCCGGCCTGT614100.5843612149727027No Hit
CTACGCCTGTCTGAGCGTCGCT602310.5731421647780631No Hit
GTCAGGATGGCCGAGCGGTCTAAGG583600.5553382267677403No Hit
CAACGGAATCCCAAAAGCAGCTGT575150.54729743167489No Hit
ACTGGACTTGGAGTCAGAAGGC510580.4858543382849089No Hit
TATTGCACTTGTCCCGGCCTGTA489930.4662043479100737No Hit
TGTAAACATCCCCGACTGGAAGCT467840.4451840918626107No Hit
CCCCCCACTGCTAAATTTGACTGGCT429110.4083296547092273No Hit
TTCACAGTGGCTAAGTTCCGC412410.39243837920028063No Hit
TTCAAGTAATTCAGGATAGGTT409630.38979300519340204No Hit
TGAGGTAGTAGTTTGTACAGTT397990.3787166910063279No Hit
TTCTCACTACTGCACTTGACTAG386780.3680495533742745No Hit
TGTAAACATCCTACACTCTCAGCT365190.3475050840186961No Hit
TGTCAGTTTGTCAAATACCCCAA355780.3385507784774274No Hit
CCCCCCACTGCTAAATTTGACTGG337240.32090860794234527No Hit
CTTCTCACTACTGCACTTGACTAG333570.31741633362391214No Hit
CGCCTGTCTGAGCGTCGCT328200.3123063845530712No Hit
GGGCTACGCCTGTCTGAGCGTCGCT327370.3115165786445427No Hit
CACCCGGCTGTGTGCACATGTGC295640.2813231551775441No Hit
CATTGCACTTGTCTCGGTCTGA283610.2698757273707999No Hit
ATCACATTGCCAGGGATTTCC279650.2661074967710736No Hit
CTCCCACTGCTTCACTTGACTAGCCT269890.25682014054548563No Hit
CTCCCACTGCTTCACTTGACTAG267620.2546600689643294No Hit
TAGCTTATCAGACTGATGTTGACA265940.25306142567959705No Hit
TACGCCTGTCTGAGCGTCGCT262140.24944544682127387No Hit
TGTCAGTTTGTCAAATACCCCA248730.2366848477449281No Hit
GCCTGTCTGAGCGTCGCT248060.23604729357780269No Hit
AACATTCAACGCTGTCGGTGAGTT246870.23491492125111726No Hit
TGAGGTAGTAGTTTGTGCTGTT239410.22781618380819857No Hit
CCCATAAAGTAGAAAGCACT228330.21727275071603516No Hit
TCAGGCTCAGTCCCCTCCCGAT219270.20865149585908566No Hit
GGAATACCGGGTGCTGTAGGCTTT212440.20215224964794162No Hit
TCTCCCAACCCTTGTACCAGTGA211340.20110551892579548No Hit
ATCACATTGCCAGGGATTTCCA208450.1983554718467023No Hit
GTCAGGATGGCCGAGTGGTCTAAGG208400.1983078931775138No Hit
ACCCCCCACTGCTAAATTTGACTG197790.18821169957572198No Hit
TCGTTTCCCGGCCAATGCACCA197290.18773591288383734No Hit
CAAAGAATTCTCCTTTTGGGCT194570.18514763327998496No Hit
TAGCAGCACGTAAATATTGGCGT185880.1768784605750301No Hit
TAGCAGCACGTAAATATTGGC185550.17656444135838623No Hit
AACGGAATCCCAAAAGCAGCTG180770.17201592058396917No Hit
TTCACAGTGGCTAAGTTCTGC178720.17006519514724217No Hit
TCTCCCAACCCTTGTACCAGTG175520.16702016031918057No Hit
TCAGGCTCAGTCCCCTCCCGATA168850.16067316584943958No Hit
TTCACAGTGGCTAAGTTCCG167340.159236290039948No Hit
TCGTACGACTCTTAGCGG165080.15708573419262947No Hit
ACTGGACTTGGAGTCAGAAGGCA164330.15637205415480254No Hit
TTCAAGTAATCCAGGATAGGC163500.15558224824627404No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC161790.15395505776002863No Hit
GGAATACCGGGTGCTGTAGGCTT158190.15052939357845926No Hit
CAACGGAATCCCAAAAGCAGCT157180.14956830446085234No Hit
CCCCCACTGCTAAATTTGACTGGCT156700.14911154923664308No Hit
TGAGGTAGTAGGTTGTGTGGTT152210.1448389847435191No Hit
CCCCCACTGCTAAATTTGACTGGCTA146390.139300827649982No Hit
AGCAGCATTGTACAGGGCTATGT146090.13901535563485123No Hit
TTCAAGTAATCCAGGATAGGCTT141500.13464763380335032No Hit
TTCAGGATGGCCGAGCGGTCTAAGG140230.13343913560596335No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT138630.13191661819193254No Hit
ACTGGACTTGGAGTCAGAAGGCT137670.13100310774351404No Hit
TGTAAACATCCTACACTCAGCT137350.1306986042607079No Hit
GCCCCCACTGCTAAATTTGACTG135050.12850998547803857No Hit
TATTGCACTTGTCCCGGCCTGTT132420.12600734747872544No Hit
ACTGGACTTGGAGTCAGAAGG131590.12521754157019696No Hit
TATTGCACTTGTCCCGGCCTGTAG130830.12449434579853232No Hit
ACGCCTGTCTGAGCGTCGCT128930.12268635636937071No Hit
TGAGGTAGTAGGTTGTATAGT126830.12068805226345526No Hit
AACCGGGCGGAAACACCA126820.12067853652961759No Hit
GTCAAGTAATCCAGGATAGGCT117940.11222856488174654No Hit
CAACACCAGTCGATGGGCTGT117380.11169568378683577No Hit
CACTAGATTGTGAGCTCCTGGAA113770.10826050387142874No Hit
AGAGGTAGTAGGTTGCATAGTT113270.1077847171795441No Hit
TGTAAACATCCCCGACTGGAAGC112250.10681411232809947No Hit
CAAAGTGCTGTTCGTGCAGGTAG111950.10652864031296869No Hit
AGCTCGGTCTGAGGCCCCTCAGT111800.10638590430540329No Hit
CACTAGATTGTGAGCTCCTGGA110200.10486338689137248No Hit
TCAGGCTCAGTCCCCTCCCGA108270.1030268502606978No Hit
TCAGGCTCAGTCCCCTCCCGAAA107700.10248445343194931No Hit
CAAAGAATTCTCCTTTTGGGCTT106460.10130450243607544No Hit
ACCGGGTGCTGTAGGCTT106140.10099999895326926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)