FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7757209
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG92620211.939887142398767No Hit
CCCCCCACTGCTAAATTTGACTG6381078.225986949687703No Hit
CCCCCACTGCTAAATTTGACTG2969713.8283227898075194No Hit
TTCAAGTAATCCAGGATAGGCT2521973.2511306579466916No Hit
TAGCAGCACGTAAATATTGGCG1804372.3260556728586272No Hit
TAGCTTATCAGACTGATGTTGAC1647712.1241015937562078No Hit
TGAGGTAGTAGATTGTATAGTT1528771.970773251049443No Hit
AACATTCAACGCTGTCGGTGAG1471801.8973318883118915No Hit
TCGTACGACTCTTAGCGG1350381.7408065194582225No Hit
AAGCTGCCAGTTGAAGAACTGT1271931.639674785093453No Hit
TAGCTTATCAGACTGATGTTGA1178631.5193995675506489No Hit
TAGCTTATCAGACTGATGTTGACT858141.1062483942356072No Hit
TATTGCACTTGTCCCGGCCTGT830441.0705396747722022No Hit
TGAGGTAGTAGGTTGTATAGTT817821.0542709368794885No Hit
GCCTGTCTGAGCGTCGCT580400.7482072482512718No Hit
TATTGCACTTGTCCCGGCCTGTAA577970.7450746782766843No Hit
AACATTCAACGCTGTCGGTGAGT576370.7430120807625527No Hit
TATTGCACTTGTCCCGGCCTGTAT563320.7261890197879161No Hit
TTCAAGTAATTCAGGATAGGTT480000.6187792542395081No Hit
TGTAAACATCCTTGACTGGAAGCT462100.5957039445501597No Hit
TTCTCACTACTGCACTTGACTAG457140.5893098922563514No Hit
CATAAAGTAGAAAGCACTACT439870.5670467303381925No Hit
TGAGGTAGTAGTTTGTACAGTT435840.5618515628494734No Hit
AGCAGCATTGTACAGGGCTATGA432170.5571204798014338No Hit
CAACGGAATCCCAAAAGCAGCTGT430170.5545422329087691No Hit
TATTGCACTTGTCCCGGCCTGTA404810.5218500623097818No Hit
CACCCGGCTGTGTGCACATGTGC329910.4252947161794919No Hit
ACCGGGTGCTGTAGGCTT329090.42423763495349937No Hit
CATTGCACTTGTCTCGGTCTGA326780.42125975979247177No Hit
ACTGGACTTGGAGTCAGAAGGC313580.40424333030088533No Hit
CAACGGAATCCCAAAAGCAGCT286170.3689084566369167No Hit
CCCCCACTGCTAAATTTGACTGGCTT268170.34570423460293515No Hit
ACCGGGTGCTGTAGGCTTT263850.3401352213147796No Hit
CCCATAAAGTAGAAAGCACTAC249820.32204881936273727No Hit
CTTCTCACTACTGCACTTGACTAG245380.31632511126102186No Hit
AACGGAATCCCAAAAGCAGCTG232600.29985011361689495No Hit
TGTAAACATCCCCGACTGGAAGCT230740.2974523440067169No Hit
TAGCTTATCAGACTGATGTTGACA225380.29054264233437566No Hit
CCCATAAAGTAGAAAGCACT193850.249896580071518No Hit
ACCCCCCACTGCTAAATTTGACTG186800.24080825977487522No Hit
CTCCCACTGCTTCACTTGACTAG176710.22780100420138222No Hit
TAGCAGCACGTAAATATTGGC175400.2261122524866869No Hit
ACCGGGTGCTGTAGGCTTA170830.22022095833694827No Hit
TTCACAGTGGCTAAGTTCCGC170750.22011782846124167No Hit
TGATGTAGTAGATTGTATAGTT169830.21893183489061596No Hit
AACCGGGCGGAAACACCA169300.21824859946405983No Hit
TGAGAACTGAATTCCATGGGTT165780.21371088493297008No Hit
TGAGAACTGAATTCCATAGGCTGT164700.2123186316109312No Hit
TGAGGTAGTAGATTGTATAGT160150.2064531199301192No Hit
CAACACCAGTCGATGGGCTGT158170.2039006555063812No Hit
CCCCCCACTGCTAAATTTGACTGG141250.18208868679443857No Hit
TGTCAGTTTGTCAAATACCCCA139490.1798198295288937No Hit
CTCCCACTGCTTCACTTGACTAGCCT137420.1771513439949858No Hit
TGTAAACATCCTACACTCTCAGCT122590.15803364328587768No Hit
CAAAGAATTCTCCTTTTGGGCT122330.1576984711898313No Hit
TCGTTTCCCGGCCAATGCACCA117790.1518458507434826No Hit
TTCACAGTGGCTAAGTTCTGC117220.1511110503790732No Hit
TATTGCACTTGTCCCGGCCTGTT117180.15105948544121992No Hit
CACTAGATTGTGAGCTCCTGGA116820.15059540100054025No Hit
TGAGGTAGTAAGTTGTATTGTT108890.14037265207112506No Hit
GGAATACCGGGTGCTGTAGGCTTT108260.13956050429993572No Hit
TTCAAGTAATCCAGGATAGGCTT102450.132070697076745No Hit
GGAATACCGGGTGCTGTAGGCTT102320.1319031110287218No Hit
CAAAGTGCTGTTCGTGCAGGTAG101790.1312198756021657No Hit
TTCAAGTAATCCAGGATAGGC100540.1296084712942503No Hit
TACCACCCTGAACGCGCCCGA97900.126205185395933No Hit
CCCATAAAGTAGAAAGCACTA96790.12477425837050414No Hit
CACTAGATTGTGAGCTCCTGGAA95720.12339489628292856No Hit
TGATGTAGTAGGTTGTATAGTT92870.1197208944608815No Hit
CCCCCCACTGCTAAATTTGACTGGCT92800.11963065581963822No Hit
TGAGGTAGTAGTTTGTGCTGTT92700.119501743475005No Hit
TGTAAACATCCCCGACTGGA92440.1191665713789586No Hit
ACTGGACTTGGAGTCAGAAGGCT91120.11746492842979994No Hit
CGCGACCTCAGATCAGACGTGGCG88960.11468042178572216No Hit
AGCTCGGTCTGAGGCCCCTCAGT87740.11310769118119675No Hit
TGGCTCAGTTCAGCAGGAAC87270.11250180316142055No Hit
AATGCACCCGGGCAAGGATTCT86610.11165098168684125No Hit
TGAGGTAGTAGATTGTATAGTTA84090.10840239060208381No Hit
ATCACATTGCCAGGGATTTCC83120.10715194085914148No Hit
AGCAGCATTGTACAGGGCTATGT82030.10574679630263926No Hit
CAAAGAATTCTCCTTTTGGGCTT82010.10572101383371262No Hit
TTATAAAGCAATGAGACTGATT81700.1053213855653496No Hit
AATCCTTGGAACCTAGGTGTGAGT81200.10467682384218344No Hit
ACTGGACTTGGAGTCAGAAGGCA77580.10001019696646049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)