FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8714649
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG115145613.212878682778847No Hit
CCCCCCACTGCTAAATTTGACTG91122910.456290322191979No Hit
CCCCCACTGCTAAATTTGACTG3388773.8885903494219907No Hit
TTCAAGTAATCCAGGATAGGCT2582522.9634240002093026No Hit
TAGCAGCACGTAAATATTGGCG1700121.9508760479050848No Hit
AACATTCAACGCTGTCGGTGAG1535441.761906876570703No Hit
TAGCTTATCAGACTGATGTTGAC1456621.6714614667785244No Hit
TGAGGTAGTAGATTGTATAGTT1253951.4388990308158136No Hit
TCGTACGACTCTTAGCGG1169721.3422456831020964No Hit
AAGCTGCCAGTTGAAGAACTGT1134741.3021063728441615No Hit
TAGCTTATCAGACTGATGTTGA928281.0651949378569348No Hit
TAGCTTATCAGACTGATGTTGACT920851.0566690637798493No Hit
TATTGCACTTGTCCCGGCCTGTAA872301.0009582715264838No Hit
TATTGCACTTGTCCCGGCCTGTAT795230.9125209747403482No Hit
TATTGCACTTGTCCCGGCCTGT727630.8349504380497712No Hit
TGAGGTAGTAGGTTGTATAGTT684170.7850803859111251No Hit
TGAGAACTGAATTCCATGGGTT608710.698490553090549No Hit
AACATTCAACGCTGTCGGTGAGT572290.6566988527019275No Hit
CAACGGAATCCCAAAAGCAGCTGT566690.6502728910825898No Hit
CCCCCACTGCTAAATTTGACTGGCTT513040.5887098837830417No Hit
TGTAAACATCCTTGACTGGAAGCT506640.5813659276466556No Hit
TTCTCACTACTGCACTTGACTAG456440.523761771701878No Hit
TTAATGCTAATCGTGATAGGGGTT453320.5201815930853898No Hit
AGCAGCATTGTACAGGGCTATGA438910.5036462168470583No Hit
TATTGCACTTGTCCCGGCCTGTA435600.4998480145327712No Hit
CACCCGGCTGTGTGCACATGTGC429180.49248110853345906No Hit
TTCAAGTAATTCAGGATAGGTT400350.4593988811253328No Hit
CAACGGAATCCCAAAAGCAGCT377670.43337373656701494No Hit
TGAGGTAGTAGTTTGTACAGTT357240.4099304515878953No Hit
ACTGGACTTGGAGTCAGAAGGC321810.369274769414121No Hit
CTTCTCACTACTGCACTTGACTAG298260.3422513058185132No Hit
CATAAAGTAGAAAGCACTACT287520.3299272294271404No Hit
ACCCCCCACTGCTAAATTTGACTG284870.3268863725894181No Hit
TGTAAACATCCCCGACTGGAAGCT277770.3187391712506149No Hit
CCCATAAAGTAGAAAGCACTAC266210.30547415047926774No Hit
CATTGCACTTGTCTCGGTCTGA265280.30440698185319914No Hit
GGAATACCGGGTGCTGTAGGCTTT235010.26967236431438607No Hit
AACGGAATCCCAAAAGCAGCTG234870.26951171527390255No Hit
TAGCTTATCAGACTGATGTTGACA232890.2672396788442082No Hit
TGAGAACTGAATTCCATAGGCTGT226600.26002194695391634No Hit
ACCGGGTGCTGTAGGCTT192860.22130552819740645No Hit
GGAATACCGGGTGCTGTAGGCTT192590.22099570504790267No Hit
CCCCCCACTGCTAAATTTGACTGG192010.2203301590230427No Hit
CTCCCACTGCTTCACTTGACTAGCCT190480.21857449450918792No Hit
CTCCCACTGCTTCACTTGACTAG190060.2180925473877376No Hit
CAAAGAATTCTCCTTTTGGGCT185660.21304357754397224No Hit
ACCGGGTGCTGTAGGCTTT173600.1992048101994699No Hit
TAGCAGCACGTAAATATTGGC170210.19531480843347793No Hit
CCCCCCACTGCTAAATTTGACTGGCT162300.18623813764616337No Hit
CCCATAAAGTAGAAAGCACT158900.18233666094870832No Hit
TCGTTTCCCGGCCAATGCACCA155430.17835485973101153No Hit
CACTAGATTGTGAGCTCCTGGA155140.17802208671858155No Hit
CACTAGATTGTGAGCTCCTGGAA150760.1729960667377424No Hit
TGATGTAGTAGATTGTATAGTT145850.16736187538935876No Hit
TTCACAGTGGCTAAGTTCCGC140220.16090148897563172No Hit
TGTAAACATCCTACACTCTCAGCT129010.14803809080549316No Hit
ACCGGGTGCTGTAGGCTTA122920.14104985754446336No Hit
TGTCAGTTTGTCAAATACCCCA121420.1393286178249979No Hit
AATGCACCCGGGCAAGGATTCT114880.13182401264812846No Hit
TGAGGTAGTAGATTGTATAGT113070.12974705005330678No Hit
TATTGCACTTGTCCCGGCCTGTT112770.1294028021094137No Hit
CCCATAAAGTAGAAAGCACTA111890.12839300814066062No Hit
ATGCACCTGGGCAAGGATTCTGA111520.1279684356765258No Hit
AATCCTTGGAACCTAGGTGTGAGT110800.1271422406111824No Hit
ACTGGACTTGGAGTCAGAAGGCT108470.12446858158027937No Hit
CAAAGAATTCTCCTTTTGGGCTT104680.12011958255576329No Hit
TTCACAGTGGCTAAGTTCTGC104570.11999335830966916No Hit
GCCTGTCTGAGCGTCGCT102470.11758362270241751No Hit
TTCAAGTAATCCAGGATAGGCTT101450.11641317969318099No Hit
TATTGCACTTGTCCCGGCCTGTAG101390.11634433010440236No Hit
CAACACCAGTCGATGGGCTGT100110.11487553887712518No Hit
AGCTCGGTCTGAGGCCCCTCAGT99870.1146001405220107No Hit
ACTGGACTTGGAGTCAGAAGGCA99060.11367067107349936No Hit
CAAAGTGCTGTTCGTGCAGGTAG98410.11292480052839764No Hit
CGCGACCTCAGATCAGACGTGGCG96970.1112724103977108No Hit
TTCAAGTAATCCAGGATAGGC94050.1079217304104847No Hit
CAAAGAATTCTCCTTTTGGGC93240.10699226096197334No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC91510.1050070978188565No Hit
TGAGGTAGTAGTTTGTGCTGTT89940.10320553357914931No Hit
TTAATGCTAATCGTGATAGGGGTTT88410.10144986906529455No Hit
ATCACATTGCCAGGGATTTCC87150.10000402770094356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)