FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7136882
Sequences flagged as poor quality0
Sequence length18-26
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG4158775.827152529634089No Hit
CAACGGAATCCCAAAAGCAGCTG3053054.2778485058320985No Hit
AAGCTGCCAGTTGAAGAACTGT2015202.823642033033473No Hit
AACATTCAACGCTGTCGGTGAGT1697912.379064134729984No Hit
TTCAAGTAATCCAGGATAGGCT1589422.227050972679666No Hit
CCCCCACTGCTAAATTTGACTG1560672.1867672745605153No Hit
TAGCAGCACGTAAATATTGGCG1310381.8360679075260036No Hit
ACCGGGTGCTGTAGGCTT1141701.59971819626554No Hit
CGCCTGTCTGAGCGTCGCT1060461.485886974171634No Hit
AACATTCAACGCTGTCGGTGAG904611.2675143010631253No Hit
GCCTGTCTGAGCGTCGCT766461.0739423742749286No Hit
ACCGGGTGCTGTAGGCTTT763141.0692904828747343No Hit
TAGCTTATCAGACTGATGTTGAC734261.0288246323814798No Hit
TATTGCACTTGTCCCGGCCTGT693820.9721612323140554No Hit
AACCGGGCGGAAACACCA679560.9521805180469566No Hit
ACCGGGTGCTGTAGGCTTA634350.8888335270220244No Hit
TCGTACGACTCTTAGCGG611130.8562983106628357No Hit
TAGCTTATCAGACTGATGTTGA594150.8325064082606382No Hit
TAGCTTATCAGACTGATGTTGACT466770.6540251050809023No Hit
CATAAAGTAGAAAGCACTACT463370.6492611199120288No Hit
TATTGCACTTGTCCCGGCCTGTAT450200.6308076832431866No Hit
TATTGCACTTGTCCCGGCCTGTAA446710.6259175925845488No Hit
TGAGGTAGTAGGTTGTATAGTT387660.5431783795780847No Hit
AGCAGCATTGTACAGGGCTATGA312340.43764209636645246No Hit
TACGCCTGTCTGAGCGTCGCT295490.41403234633835895No Hit
CTACGCCTGTCTGAGCGTCGCT295360.413850193964255No Hit
TATTGCACTTGTCCCGGCCTGTA292550.4099129003393919No Hit
TGTAAACATCCTTGACTGGAAGCT292550.4099129003393919No Hit
TTCAAGTAATTCAGGATAGGTT286190.40100144572938157No Hit
ACGCCTGTCTGAGCGTCGCT280620.39319691708508003No Hit
ACTGGACTTGGAGTCAGAAGGC277150.3883348498686121No Hit
TGAGGTAGTAGATTGTATAGTT246520.3454169481854961No Hit
TTCTCACTACTGCACTTGACTAG226460.3173094356891427No Hit
CCCATAAAGTAGAAAGCACTAC219500.30755727781403697No Hit
GCGTTCGCGCTTTCCCCTG218320.3059038947260162No Hit
TTAATGCTAATCGTGATAGGGGTT216410.3032276559987961No Hit
CAACGGAATCCCAAAAGCAGCTGT205940.28855738402288283No Hit
CATTGCACTTGTCTCGGTCTGA201710.2826304260039608No Hit
CGGCCCCGCCGGGGTCGGC195600.2740692644210735No Hit
ATCCCGGACGAGCCCCCA180670.25314976484128504No Hit
CCCATAAAGTAGAAAGCACT168080.23550900799536828No Hit
TGTAAACATCCCCGACTGGAAGCT163450.2290215811330494No Hit
CACCCGGCTGTGTGCACATGTGC154870.2169995244421864No Hit
CGCGACCTCAGATCAGACGTGG143340.20084401003127136No Hit
TGTAAACATCCTACACTCTCAGCT139490.1954494974135764No Hit
CTCCCACTGCTTCACTTGACTAG138410.19393623153640482No Hit
CAACACCAGTCGATGGGCTGT136040.19061545363927831No Hit
GGGCTACGCCTGTCTGAGCGTCGCT135910.19043330126517433No Hit
AACATTCAACGCTGTCGGTGAGTT128200.17963026430869952No Hit
CTTCTCACTACTGCACTTGACTAG126080.17665977943869607No Hit
ATCCCACCGCTGCCACCA122920.17223207557586073No Hit
GGAATACCGGGTGCTGTAGGCTTT111630.1564128424709838No Hit
GGAATACCGGGTGCTGTAGGCTT108780.15241950196178108No Hit
CGCGACCTCAGATCAGAC108570.15212525581899772No Hit
AGCTCGGTCTGAGGCCCCTCAGT108480.15199915032923342No Hit
CTCGACACAAGGGTTTGT108300.15174693934970482No Hit
TACCTGGTTGATCCTGCC106020.1485522669423426No Hit
CGCGACCTCAGATCAGACGTGGCG101370.1420368166378539No Hit
TGAGGTAGTAGTTTGTACAGTT101110.1416725118896459No Hit
ACCTGGTGCTGTAGGCTT98780.1384077808768591No Hit
CCCCCACTGCTAAATTTGACTGGCTT98700.13829568710817972No Hit
CCCCCCACTGCTAAATTTGACTGG96930.13581561247614857No Hit
TATTGCACTTGTCCCGGCCTGTT94710.13270501039529586No Hit
TGAGGTAGTAGTTTGTGCTGTT91130.1276888142468938No Hit
CCTCGACACAAGGGTTTGT90960.12745061498845014No Hit
GCTCCCCGTCCTCCCCCCT90310.12653985311793023No Hit
TTCAAGTAATCCAGGATAGGC88430.1239056495539649No Hit
CCGGGTGCTGTAGGCTTT87630.12278471186717113No Hit
CTGGACGCGAGCCGGGCCCT87300.12232232507136871No Hit
AACGGAATCCCAAAAGCAGCTG86820.12164976245929245No Hit
ACGCGACCTCAGATCAGA86310.12093516468396143No Hit
TTCAAGTAATCCAGGATAGGCTT84820.11884741824230804No Hit
TGTAAACATCCTACACTCAGCT83890.1175443281814103No Hit
CAACGGAATCCCAAAAGCAGCT83500.11699787105909835No Hit
CCCCCCACTGCTAAATTTGACTGGCT80260.11245807342758363No Hit
ACCCCCCACTGCTAAATTTGACTG79570.11149126467272404No Hit
ATACCGGGTGCTGTAGGCTT78690.11025823321725088No Hit
TGTCAGTTTGTCAAATACCCCAA78160.10951561199975003No Hit
TTCACAGTGGCTAAGTTCCG75450.10571843558573618No Hit
TTATAAAGCAATGAGACTGATT72510.10159898958676912No Hit
TGTCAGTTTGTCAAATACCCCA71630.10036595813129598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)