FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11358338
Sequences flagged as poor quality0
Sequence length18-26
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG199297317.54634348792931No Hit
CCCCCACTGCTAAATTTGACTG5625484.9527316408439335No Hit
CAACGGAATCCCAAAAGCAGCTG5617654.945838026654957No Hit
TTCAAGTAATCCAGGATAGGCT2986242.629117041595346No Hit
AACATTCAACGCTGTCGGTGAGT2820362.483074548406642No Hit
TAGCAGCACGTAAATATTGGCG2434372.143244900794465No Hit
AAGCTGCCAGTTGAAGAACTGT2183521.9223939277031552No Hit
GCCTGTCTGAGCGTCGCT1812071.595365448712655No Hit
AACATTCAACGCTGTCGGTGAG1578151.3894198253300791No Hit
TAGCTTATCAGACTGATGTTGAC1388331.2223003048509384No Hit
TAGCTTATCAGACTGATGTTGACT993740.8748991269673432No Hit
ACCGGGTGCTGTAGGCTT969780.8538044914669733No Hit
TAGCTTATCAGACTGATGTTGA924750.814159606801629No Hit
TGAGGTAGTAGGTTGTATAGTT719240.6332264456296335No Hit
TGTAAACATCCTTGACTGGAAGCT681470.5999733411701607No Hit
TATTGCACTTGTCCCGGCCTGT667460.5876387901117224No Hit
CCCCCACTGCTAAATTTGACTGGCTT639310.5628552346302778No Hit
TATTGCACTTGTCCCGGCCTGTAA625870.5510225175549451No Hit
TATTGCACTTGTCCCGGCCTGTAT592720.5218369095901179No Hit
CGCCTGTCTGAGCGTCGCT576380.5074510020744232No Hit
ACCGGGTGCTGTAGGCTTT548050.48250897270357684No Hit
CATAAAGTAGAAAGCACTACT543820.47878483630263513No Hit
AGCAGCATTGTACAGGGCTATGA460320.40527055983014415No Hit
TGAGGTAGTAGATTGTATAGTT423440.3728010207127134No Hit
ACCGGGTGCTGTAGGCTTA413280.3638560500664798No Hit
TATTGCACTTGTCCCGGCCTGTA403390.3551487902543488No Hit
CCCATAAAGTAGAAAGCACTAC371830.32736303497923724No Hit
CTACGCCTGTCTGAGCGTCGCT362920.3195185774538493No Hit
TTCTCACTACTGCACTTGACTAG356310.31369906407081743No Hit
TTCAAGTAATTCAGGATAGGTT353840.31152445014402635No Hit
CCCCCCACTGCTAAATTTGACTGG349290.3075185823841481No Hit
ACTGGACTTGGAGTCAGAAGGC323270.2846103012606246No Hit
CAACGGAATCCCAAAAGCAGCTGT315790.27802483074548406No Hit
TTAATGCTAATCGTGATAGGGGTT303180.2669228543823929No Hit
TGTAAACATCCCCGACTGGAAGCT302350.2661921136701514No Hit
CCCCCCACTGCTAAATTTGACTGGCT292030.2571062773444495No Hit
TACGCCTGTCTGAGCGTCGCT274160.24137334176883976No Hit
AACCGGGCGGAAACACCA272580.23998229318409084No Hit
ACCCCCCACTGCTAAATTTGACTG258280.22739242308161636No Hit
GGGCTACGCCTGTCTGAGCGTCGCT252180.22202191905188948No Hit
CATTGCACTTGTCTCGGTCTGA238040.2095729146288832No Hit
TGTAAACATCCTACACTCTCAGCT234100.20610409727197765No Hit
CGCGACCTCAGATCAGACGTGGCG223160.1964724064383363No Hit
GGAATACCGGGTGCTGTAGGCTTT220630.19424496788174467No Hit
GGAATACCGGGTGCTGTAGGCTT216900.1909610367291412No Hit
AACATTCAACGCTGTCGGTGAGTT214490.18883924743215072No Hit
TGAGGTAGTAGTTTGTACAGTT209590.18452523599843568No Hit
CTTCTCACTACTGCACTTGACTAG193930.1707380076204811No Hit
TAGCTTATCAGACTGATGTTGACA192370.16936456724566568No Hit
ACGCCTGTCTGAGCGTCGCT181990.16022590629016323No Hit
CAACGGAATCCCAAAAGCAGCT181090.15943353684315434No Hit
CACCCGGCTGTGTGCACATGTGC174550.15367565219488977No Hit
TCGTACGACTCTTAGCGG171580.15106083301976045No Hit
CGCGACCTCAGATCAGACGTGG169380.14912392992707207No Hit
GTCAGGATGGCCGAGCGGTCTAAGG168940.14873654930853442No Hit
TGAGGTAGTAGTTTGTGCTGTT167200.14720463504431722No Hit
TTCACAGTGGCTAAGTTCCG161170.1418957597493577No Hit
TGTCAGTTTGTCAAATACCCCAA159200.1401613510709049No Hit
TTCACAGTGGCTAAGTTCCGC158640.13966832119276604No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT155470.1368774199183014No Hit
TCCACCACGTTCCCGTGG154150.1357152780626884No Hit
CAACACCAGTCGATGGGCTGT149840.1319207088220125No Hit
CTCCCACTGCTTCACTTGACTAG139830.12310779975028036No Hit
CAAAGAATTCTCCTTTTGGGCT139450.12277324376154329No Hit
TAGCAGCACGTAAATATTGGC134690.11858248979736295No Hit
GTGCGGTAACGCGACCGATCCCGGA133850.11784294498015466No Hit
TATTGCACTTGTCCCGGCCTGTT131910.11613494861660219No Hit
GACCCGCGGGCGCTCTCCAGTCCT131440.1157211556831642No Hit
CCCATAAAGTAGAAAGCACT125670.11064118711734058No Hit
CTCCCACTGCTTCACTTGACTAGCCT125630.11060597069747352No Hit
AACGGAATCCCAAAAGCAGCTG124450.10956708631139521No Hit
TGTCAGTTTGTCAAATACCCCA123660.10887156201902075No Hit
TAGCAGCACATCATGGTTTACA120460.10605424842965581No Hit
TGTAAACATCCTACACTCAGCT120450.10604544432468906No Hit
TAGCAGCACGTAAATATTGGCGT119890.10555241444655018No Hit
CGGCCCCGCCGGGGTCGGC119180.10492732299390985No Hit
TTCAAGTAATCCAGGATAGGC114810.10107992912343337No Hit
GCTCCCCGTCCTCCCCCCT114460.1007717854495966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)