FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7569567
Sequences flagged as poor quality0
Sequence length18-26
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG152186820.105086592139287No Hit
CAACGGAATCCCAAAAGCAGCTG6615218.739218504836538No Hit
CCCCCACTGCTAAATTTGACTG4293035.671434046359587No Hit
TTCAAGTAATCCAGGATAGGCT1753082.3159580990563926No Hit
AACATTCAACGCTGTCGGTGAGT1082681.4303063834430687No Hit
AACATTCAACGCTGTCGGTGAG590620.7802559908644708No Hit
ACTGGACTTGGAGTCAGAAGGC549090.7253915580640213No Hit
TAGCAGCACGTAAATATTGGCG508390.671623621271864No Hit
CCCCCACTGCTAAATTTGACTGGCTT504830.6669205781519604No Hit
CCCATAAAGTAGAAAGCACTAC413190.5458568502002823No Hit
ACCGGGTGCTGTAGGCTTA406720.5373094656537157No Hit
AAGCTGCCAGTTGAAGAACTGT403880.5335575997940173No Hit
CAACGGAATCCCAAAAGCAGCTGT402110.5312192890293461No Hit
CTCCCACTGCTTCACTTGACTAG376420.49728075595341187No Hit
AGCAGCATTGTACAGGGCTATGA332070.4386908788838252No Hit
CACCCGGCTGTGTGCACATGTGC325100.42948295457322727No Hit
CCCCCCACTGCTAAATTTGACTGGCT311910.4120579155980785No Hit
ACCGGGTGCTGTAGGCTT310530.4102348258493518No Hit
CCCCCCACTGCTAAATTTGACTGG298840.3947914061662972No Hit
CATAAAGTAGAAAGCACTACT298190.3939327044730564No Hit
CTTCTCACTACTGCACTTGACTAG252450.33350652685946236No Hit
CAACGGAATCCCAAAAGCAGCT248770.32864495419619116No Hit
ACCGGGTGCTGTAGGCTTT248500.32828826272361417No Hit
CGCCTGTCTGAGCGTCGCT234510.30980636012601515No Hit
TCGTACGACTCTTAGCGG233710.30874949650356487No Hit
ACCCCCCACTGCTAAATTTGACTG231760.3061733914238423No Hit
GCCTGTCTGAGCGTCGCT228410.30174777500483185No Hit
CACTAGATTGTGAGCTCCTGGAA218860.2891314655118318No Hit
TAGCTTATCAGACTGATGTTGA210850.2785496184920485No Hit
TAGCTTATCAGACTGATGTTGAC205240.27113836233961597No Hit
GACCCGCGGGCGCTCTCCAGTCCT201190.26578799025096156No Hit
CACTAGATTGTGAGCTCCTGGA200570.26496892094356256No Hit
CGCGACCTCAGATCAGACGTGGC191050.2523922438364044No Hit
CCCATAAAGTAGAAAGCACT181750.24010620422542003No Hit
CTACGCCTGTCTGAGCGTCGCT181490.23976272354812367No Hit
CATTGCACTTGTCTCGGTCTGA179540.2371866184684012No Hit
AGCTCGGTCTGAGGCCCCTCAGT178060.23523142076686815No Hit
CAAAGAATTCTCCTTTTGGGCT174550.23059443162336762No Hit
CAACACCAGTCGATGGGCTGT168470.2225622680927456No Hit
CGCGACCTCAGATCAGACGTGGCG165490.21862545109911838No Hit
TTCTCACTACTGCACTTGACTAG163320.21575870852322201No Hit
ACTGGACTTGGAGTCAGAAGGCT160330.21180868073431414No Hit
TTCAAGTAATTCAGGATAGGTT154500.20410678708570779No Hit
CGCGACCTCAGATCAGACGT134450.177619142548048No Hit
TACGCCTGTCTGAGCGTCGCT133460.1763112738152658No Hit
CAAAGAATTCTCCTTTTGGGCTT127660.16864901255250134No Hit
AACCGGGCGGAAACACCA127140.1679620511979087No Hit
TTCAAGTAATCCAGGATAGGCTT120450.15912402915516832No Hit
AACGGAATCCCAAAAGCAGCTG115470.1525450531054154No Hit
CCCCCCACTGCTAAATTTGACTGA115080.1520298320894709No Hit
ACTGGACTTGGAGTCAGAAGGCA110100.145450856039718No Hit
TGTAAACATCCTACACTCTCAGCT109580.14476389468512532No Hit
CCCCCACTGCTAAATTTGACTGGCT109250.14432793844086458No Hit
TATTGCACTTGTCCCGGCCTGTAA108120.1428351185741536No Hit
ACTGGACTTGGAGTCAGAAGG104380.1378942811391986No Hit
ATAGGCGATAGAAATTGAAACCT99100.13091898123102683No Hit
TAGCTTATCAGACTGATGTTGACT98930.13069439771125613No Hit
CCCCCCACTGCTAAATTTGAC98560.1302055982858729No Hit
GCGGGCCGCCGGTGAAATACCACTAC90820.11998044273866657No Hit
AACATTCAACGCTGTCGGTGAGTT88470.1168759058477189No Hit
TATTGCACTTGTCCCGGCCTGTAT85500.11295229964937228No Hit
CCCATAAAGTAGAAAGCACTA83040.10970244401033771No Hit
GGAATACCGGGTGCTGTAGGCTTT81590.10778687869464659No Hit
CTCCCACTGCTTCACTTGACTAGCCT76510.10107579469208741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)