FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4561687
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG1980534.341661319595141No Hit
GCCTGTCTGAGCGTCGCT1807463.9622622069422997No Hit
CCCCCCACTGCTAAATTTGACTG1658033.634686027340324No Hit
TTCAAGTAATCCAGGATAGGCT885861.9419569996801622No Hit
TCGTACGACTCTTAGCGG839061.8393633758738819No Hit
AAGCTGCCAGTTGAAGAACTGT778811.7072850460805398No Hit
AACATTCAACGCTGTCGGTGAGT747031.6376178374360186No Hit
TAGCTTATCAGACTGATGTTGAC736741.6150603932273304No Hit
TAGCAGCACGTAAATATTGGCG701641.5381151753726199No Hit
CCCCCACTGCTAAATTTGACTG648681.42201777544141No Hit
AACATTCAACGCTGTCGGTGAG535421.173732437144416No Hit
TATTGCACTTGTCCCGGCCTGT428220.938731657827466No Hit
TAGCTTATCAGACTGATGTTGA418370.9171387690562725No Hit
TAGCTTATCAGACTGATGTTGACT353490.7749106854547452No Hit
TGAGGTAGTAGGTTGTATAGTT304970.668546526756439No Hit
ACCGGGTGCTGTAGGCTT297520.6522148494624905No Hit
CATAAAGTAGAAAGCACTACT277460.6082398901985164No Hit
TGTAAACATCCTTGACTGGAAGCT240750.5277652763111542No Hit
TATTGCACTTGTCCCGGCCTGTAA238860.5236220722728236No Hit
TTCAAGTAATTCAGGATAGGTT237080.5197200070938668No Hit
TATTGCACTTGTCCCGGCCTGTAT214020.4691685334833363No Hit
TGAGGTAGTAGATTGTATAGTT201850.44248980695080575No Hit
ACCGGGTGCTGTAGGCTTT195400.4283503011057094No Hit
AGCAGCATTGTACAGGGCTATGA188410.4130270226782328No Hit
CGCCTGTCTGAGCGTCGCT181800.3985367694013202No Hit
TATTGCACTTGTCCCGGCCTGTA179850.39426203507605845No Hit
TGTAAACATCCCCGACTGGAAGCT161160.35329035069701187No Hit
ACTGGACTTGGAGTCAGAAGGC153430.3363448653973848No Hit
CATTGCACTTGTCTCGGTCTGA146540.3212408041147935No Hit
TTAATGCTAATCGTGATAGGGGTT145130.31814984237191196No Hit
CAACGGAATCCCAAAAGCAGCT138330.3032430765197174No Hit
ACCGGGTGCTGTAGGCTTA132070.28952008324990297No Hit
TCCTGTCTGAGCGTCGCT118310.2593558041136974No Hit
CACCCGGCTGTGTGCACATGTGC116350.2550591480739472No Hit
CCCATAAAGTAGAAAGCACTAC112020.24556704570041743No Hit
CAACGGAATCCCAAAAGCAGCTGT109730.240546973082546No Hit
CCCCCACTGCTAAATTTGACTGGCTT101130.22169429862241752No Hit
TGAGGTAGTAGTTTGTGCTGTT100240.21974326603293914No Hit
CAACACCAGTCGATGGGCTGT94840.20790554020913757No Hit
TAGCTTATCAGACTGATGTTGACA90300.1979530818313488No Hit
CGCGACCTCAGATCAGACGTGG86390.1893816914663369No Hit
TGAGGTAGTAGTTTGTACAGTT83870.18385741941522948No Hit
TTCTCACTACTGCACTTGACTAG81390.17842083422207616No Hit
CCCATAAAGTAGAAAGCACT80640.17677670563543704No Hit
TCGTTTCCCGGCCAATGCACCA79480.17423378675476855No Hit
CTCCCACTGCTTCACTTGACTAG77740.17041940843376585No Hit
TGAGAACTGAATTCCATGGGTT76160.16695577754457944No Hit
CCCCCCACTGCTAAATTTGACTGGCT75340.16515819695652068No Hit
AACCGGGCGGAAACACCA72320.1585378391809872No Hit
CCCCCCACTGCTAAATTTGACTGG70440.15441655685714517No Hit
CTACGCCTGTCTGAGCGTCGCT67850.1487388328046181No Hit
AACATTCAACGCTGTCGGTGAGTT67140.1471823910759331No Hit
CGCGACCTCAGATCAGACGTGGCG65450.14347762132737296No Hit
TAGCAGCACGTAAATATTGGC64270.14089085901772744No Hit
TATTGCACTTGTCCCGGCCTGTT62400.13679149840837393No Hit
TGAGGTAGTAGGTTGTGTGGTT59850.13120146121380097No Hit
ATCCCACCGCTGCCACCA55400.12144629826640889No Hit
TGTAAACATCCTACACTCTCAGCT54160.11872800566983224No Hit
ACGGACCAGAGCGAAAGCATT54080.11855263195392407No Hit
TACGCCTGTCTGAGCGTCGCT53560.11741270280052095No Hit
TTATAAAGCAATGAGACTGATT53070.11633853879058341No Hit
GGAATACCGGGTGCTGTAGGCTT52540.11517668792269177No Hit
AACGGAATCCCAAAAGCAGCTG51680.11329142047667891No Hit
AGCTCGGTCTGAGGCCCCTCAGT51490.11287490790139701No Hit
GGAATACCGGGTGCTGTAGGCTTT51480.11285298618690849No Hit
TTCACCACCTTCTCCACCCAGC49350.10818366100085341No Hit
CTTCTCACTACTGCACTTGACTAG48960.10732871413580108No Hit
CAAAGAATTCTCCTTTTGGGCT48590.10651761069972578No Hit
TTCAAGTAATCCAGGATAGGCTT48040.1053119164028571No Hit
GGGAATACCGGGTGCTGTAGGCTT47510.10415006553496546No Hit
TGTCAGTTTGTCAAATACCCCAA47420.10395277010456878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)