FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6869881
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG70049010.196537611059057No Hit
CAACGGAATCCCAAAAGCAGCTG4722286.873889081921507No Hit
CCCCCACTGCTAAATTTGACTG2448313.563831746139416No Hit
AAGCTGCCAGTTGAAGAACTGT2038092.9667034989397925No Hit
TTCAAGTAATCCAGGATAGGCT2013862.9314336012516082No Hit
AACATTCAACGCTGTCGGTGAGT1987352.8928448687830253No Hit
GCCTGTCTGAGCGTCGCT1956942.8485791820848134No Hit
TAGCAGCACGTAAATATTGGCG1727462.5145413726962667No Hit
AACATTCAACGCTGTCGGTGAG1236551.7999583981149019No Hit
TAGCTTATCAGACTGATGTTGAC979691.426065458775778No Hit
TGAGGTAGTAGGTTGTATAGTT882671.2848403050940767No Hit
TAGCTTATCAGACTGATGTTGACT829551.207517277227946No Hit
TATTGCACTTGTCCCGGCCTGTAA747701.0883740198702132No Hit
TATTGCACTTGTCCCGGCCTGTAT673290.9800606444274653No Hit
TAGCTTATCAGACTGATGTTGA665650.9689396366545505No Hit
TATTGCACTTGTCCCGGCCTGT532900.7757048484537068No Hit
TCCTGTCTGAGCGTCGCT516930.7524584486980197No Hit
TGTAAACATCCTTGACTGGAAGCT514200.7484845807372792No Hit
TGAGGTAGTAGATTGTATAGTT472570.6878867334092104No Hit
CATAAAGTAGAAAGCACTACT414560.6034456783166987No Hit
AGCAGCATTGTACAGGGCTATGA335640.4885674147776358No Hit
TATTGCACTTGTCCCGGCCTGTA331870.4830796923556609No Hit
ACCGGGTGCTGTAGGCTT322940.4700809227990994No Hit
TGTAAACATCCCCGACTGGAAGCT314580.45791186193763767No Hit
CGCCTGTCTGAGCGTCGCT306360.4459465891767266No Hit
TTCAAGTAATTCAGGATAGGTT302220.4399202839175817No Hit
AACCGGGCGGAAACACCA300320.4371545882672494No Hit
CAACGGAATCCCAAAAGCAGCTGT297860.433573740214714No Hit
ACTGGACTTGGAGTCAGAAGGC291880.4248690770626158No Hit
ACCGGGTGCTGTAGGCTTT278950.40604779034746014No Hit
TTCTCACTACTGCACTTGACTAG266740.3882745567208515No Hit
ACCGGGTGCTGTAGGCTTA265400.38632401347272244No Hit
CCCCCACTGCTAAATTTGACTGGCTT246440.35872528214098615No Hit
CACCCGGCTGTGTGCACATGTGC233780.34029701533403567No Hit
CCCATAAAGTAGAAAGCACTAC217210.3161772380045593No Hit
CAACGGAATCCCAAAAGCAGCT212730.30965601878693383No Hit
TCGTACGACTCTTAGCGG200360.29164988447398144No Hit
TTAATGCTAATCGTGATAGGGGTT199530.2904417121635731No Hit
CCCCCCACTGCTAAATTTGACTGG190550.27737016114252927No Hit
CATTGCACTTGTCTCGGTCTGA183710.2674136568013332No Hit
TGAGGTAGTAGTTTGTACAGTT179070.260659536897364No Hit
CTTCTCACTACTGCACTTGACTAG178910.26042663621102025No Hit
CCCATAAAGTAGAAAGCACT162380.23636508405312986No Hit
CCCCCCACTGCTAAATTTGACTGGCT159690.2324494412639753No Hit
CTCCCACTGCTTCACTTGACTAG153550.2235118774255333No Hit
AACATTCAACGCTGTCGGTGAGTT148130.2156223666756382No Hit
CTACGCCTGTCTGAGCGTCGCT145970.21247820740999734No Hit
CAACACCAGTCGATGGGCTGT144370.21014920054655967No Hit
AACGGAATCCCAAAAGCAGCTG131570.1915171456390584No Hit
ACCCCCCACTGCTAAATTTGACTG129710.1888096751603121No Hit
TAGCTTATCAGACTGATGTTGACA127990.1863059927821166No Hit
TGTAAACATCCTACACTCTCAGCT127280.18527249598646614No Hit
AGCTCGGTCTGAGGCCCCTCAGT126610.18429722436240162No Hit
TAGCAGCACGTAAATATTGGC121110.17629126326933464No Hit
TGAGAACTGAATTCCATGGGTT120730.1757381241392682No Hit
TACGCCTGTCTGAGCGTCGCT120140.17487930285837555No Hit
TGAGGTAGTAGTTTGTGCTGTT118140.1719680442790785No Hit
CAAAGAATTCTCCTTTTGGGCT108080.15732441362521418No Hit
TTCAAGTAATCCAGGATAGGCTT102200.14876531340208077No Hit
CTCCCACTGCTTCACTTGACTAGCCT101960.1484159623725651No Hit
CGCGACCTCAGATCAGACGTGG99750.14519902164244183No Hit
GGAATACCGGGTGCTGTAGGCTTT99000.14410729967520544No Hit
TGTAAACATCCCCGACTGGAAGC94980.1382556699308183No Hit
CAAAGAATTCTCCTTTTGGGCTT92420.13452925894931805No Hit
TATTGCACTTGTCCCGGCCTGTT90080.13112308641154047No Hit
CACTAGATTGTGAGCTCCTGGA88460.12876496696230982No Hit
TGAGGTAGTAGGTTGTGTGGTT87990.128080821196175No Hit
ACGCCTGTCTGAGCGTCGCT87530.1274112317229367No Hit
CGCGACCTCAGATCAGACGTGGCG87120.12681442371418078No Hit
TATTGCACTTGTCCCGGCCTGTAG87080.12675619854259484No Hit
TTCAAGTAATCCAGGATAGGC85990.12516956261687792No Hit
TTCACAGTGGCTAAGTTCTGC81670.11888124408559625No Hit
GGAATACCGGGTGCTGTAGGCTT80250.11681425049429532No Hit
GACCCGCGGGCGCTCTCCAGTCCT77100.11222901823190243No Hit
CAACGGAATCCCAAAAGCAGCTGA75080.10928864706681235No Hit
CACTAGATTGTGAGCTCCTGGAA75060.10925953448101938No Hit
AATGCACCCGGGCAAGGATTCT74960.10911397155205455No Hit
CCCTGTCTGAGCGTCGCT74930.10907030267336508No Hit
TTATAAAGCAATGAGACTGATT74400.10829881914985137No Hit
TCGTTTCCCGGCCAATGCACCA74040.10777479260557789No Hit
TGAGGTAGTAGGTTGTATAGT73590.10711975942523605No Hit
GGGAATACCGGGTGCTGTAGGCTTT71970.10476163997600539No Hit
ACTGGACTTGGAGTCAGAAGGCT71770.10447051411807569No Hit
GGGAATACCGGGTGCTGTAGGCTT69620.10134091114533134No Hit
TAGCAGCACGTAAATATTGGCGT69570.10126812968084892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)