FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10767458
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG126305511.730298831906287No Hit
CAACGGAATCCCAAAAGCAGCTG7751867.199340828633834No Hit
CCCCCACTGCTAAATTTGACTG3755093.4874433687133957No Hit
TAGCAGCACGTAAATATTGGCG3459203.212643132668825No Hit
TTCAAGTAATCCAGGATAGGCT3392953.1511151471405783No Hit
AAGCTGCCAGTTGAAGAACTGT2511912.332871881181241No Hit
AACATTCAACGCTGTCGGTGAGT2345492.1783135815342862No Hit
GCCTGTCTGAGCGTCGCT2161732.0076512023543533No Hit
AACATTCAACGCTGTCGGTGAG1867601.7344855210951367No Hit
TATTGCACTTGTCCCGGCCTGTAA1244071.1553980521679303No Hit
TAGCTTATCAGACTGATGTTGAC1223241.13605272479354No Hit
TATTGCACTTGTCCCGGCCTGTAT1155171.0728344610213478No Hit
TAGCTTATCAGACTGATGTTGACT1151361.06929602140078No Hit
TGAGGTAGTAGGTTGTATAGTT1119301.039521120026658No Hit
TAGCTTATCAGACTGATGTTGA882430.8195341927500438No Hit
TGTAAACATCCTTGACTGGAAGCT767300.7126101629558248No Hit
TATTGCACTTGTCCCGGCCTGT721280.6698702702160528No Hit
TGAGGTAGTAGATTGTATAGTT646330.6002623831920217No Hit
CATAAAGTAGAAAGCACTACT596910.5543648277987246No Hit
TTCAAGTAATTCAGGATAGGTT592850.5505942071006917No Hit
TTCTCACTACTGCACTTGACTAG577170.5360318099220819No Hit
CCCCCACTGCTAAATTTGACTGGCTT572200.531416050102076No Hit
TATTGCACTTGTCCCGGCCTGTA544820.5059875784981005No Hit
CAACGGAATCCCAAAAGCAGCTGT517780.4808748731594774No Hit
AGCAGCATTGTACAGGGCTATGA486360.4516943553436661No Hit
TTAATGCTAATCGTGATAGGGGTT467670.43433649799237667No Hit
CTCCCACTGCTTCACTTGACTAG424640.39437349093908697No Hit
TGTAAACATCCCCGACTGGAAGCT411980.38261584117625536No Hit
ACCGGGTGCTGTAGGCTTA411760.3824115218280861No Hit
CCCCCCACTGCTAAATTTGACTGG389810.362026023226652No Hit
ACCGGGTGCTGTAGGCTT372580.34602410336775863No Hit
ACCGGGTGCTGTAGGCTTT362040.33623534914183084No Hit
CCCATAAAGTAGAAAGCACTAC361200.3354552207215482No Hit
CTTCTCACTACTGCACTTGACTAG342200.3178094588342021No Hit
CCCCCCACTGCTAAATTTGACTGGCT339750.3155340842750443No Hit
ACTGGACTTGGAGTCAGAAGGC337850.31376950808630966No Hit
CACCCGGCTGTGTGCACATGTGC323090.30006153727277135No Hit
ACCCCCCACTGCTAAATTTGACTG298180.2769270147141507No Hit
AACCGGGCGGAAACACCA272290.252882342331867No Hit
TGAGAACTGAATTCCATGGGTT257280.23894219044086357No Hit
CATTGCACTTGTCTCGGTCTGA256810.2385056900152292No Hit
TGAGGTAGTAGTTTGTACAGTT241360.22415689942788725No Hit
GGAATACCGGGTGCTGTAGGCTTT239080.2220394080014057No Hit
CAAAGAATTCTCCTTTTGGGCT229770.2133929846766061No Hit
TAGCAGCACGTAAATATTGGC224840.2088143738289947No Hit
CTCCCACTGCTTCACTTGACTAGCCT216530.20109667481405546No Hit
AGCTCGGTCTGAGGCCCCTCAGT211660.1965737874250357No Hit
GGGAATACCGGGTGCTGTAGGCTTT201550.18718438465234785No Hit
GGAATACCGGGTGCTGTAGGCTT201000.18667358628192465No Hit
TGTAAACATCCTACACTCTCAGCT200040.18578201094445876No Hit
GGGAATACCGGGTGCTGTAGGCTT199410.18519691462924676No Hit
CGCCTGTCTGAGCGTCGCT190450.17687554481289827No Hit
CTACGCCTGTCTGAGCGTCGCT188960.1754917455912064No Hit
AACATTCAACGCTGTCGGTGAGTT184200.17107101787627127No Hit
TAGCTTATCAGACTGATGTTGACA182200.16921356925655062No Hit
AACGGAATCCCAAAAGCAGCTG181900.1689349519635925No Hit
CCCATAAAGTAGAAAGCACT176020.16347405302161383No Hit
GACCCGCGGGCGCTCTCCAGTCCT175510.16300040362358506No Hit
TTCAAGTAATCCAGGATAGGC166510.15464188483484215No Hit
GAATACCGGGTGCTGTAGGCTTT153940.14296782025989793No Hit
TATTGCACTTGTCCCGGCCTGTAG151080.1403116687336974No Hit
TGAGGTAGTAGTTTGTGCTGTT145680.1352965574604517No Hit
CAACGGAATCCCAAAAGCAGCT143520.13329051295115338No Hit
CAACACCAGTCGATGGGCTGT141430.1313494791435453No Hit
TATTGCACTTGTCCCGGCCTGTT140420.13041146759058636No Hit
TTCAAGTAATCCAGGATAGGCTT139930.12995639267875483No Hit
CAAAGAATTCTCCTTTTGGGCTT134070.12451406822297333No Hit
CCCCCACTGCTAAATTTGACTGGCT130420.12112422449198315No Hit
CGCGACCTCAGATCAGACGTGG128830.11964755283930524No Hit
CGCGACCTCAGATCAGACGTGGCG126310.11730716757845723No Hit
TGAGGTAGTAGGTTGTGTGGTT126100.11711213547338657No Hit
TGATGTAGTAGGTTGTATAGTT123270.11448384567648184No Hit
CACTAGATTGTGAGCTCCTGGA121370.11271926948774726No Hit
TGTAAACATCCCCGACTGGAAGC120320.11174410896239392No Hit
TTCACAGTGGCTAAGTTCCG120040.11148406615563301No Hit
CACTAGATTGTGAGCTCCTGGAA115120.10691474255112024No Hit
TCCCACTGCTTCACTTGACTAG115080.10687759357872584No Hit
GAATACCGGGTGCTGTAGGCTT114760.10658040179957053No Hit
CCCCCACTGCTAAATTTGACTGG111380.10344131363224263No Hit
TACGCCTGTCTGAGCGTCGCT108350.10062727897336586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)