FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6848015
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6420339.375461356320043No Hit
CAACGGAATCCCAAAAGCAGCTG5589498.162204668068046No Hit
CCCCCACTGCTAAATTTGACTG2554313.730000591412256No Hit
TTCAAGTAATCCAGGATAGGCT2199643.212084085680303No Hit
AAGCTGCCAGTTGAAGAACTGT2077543.033784242587086No Hit
TAGCAGCACGTAAATATTGGCG1994202.912084742805032No Hit
GCCTGTCTGAGCGTCGCT1973222.881448127669113No Hit
AACATTCAACGCTGTCGGTGAGT1552182.2666130258184305No Hit
AACATTCAACGCTGTCGGTGAG1144861.6718129268116382No Hit
TGAGGTAGTAGGTTGTATAGTT984441.4375552623643495No Hit
TAGCTTATCAGACTGATGTTGAC962931.406144700325569No Hit
TAGCTTATCAGACTGATGTTGACT811551.185087941542184No Hit
TCCTGTCTGAGCGTCGCT799501.167491601580896No Hit
TAGCTTATCAGACTGATGTTGA735951.0746909870962609No Hit
TATTGCACTTGTCCCGGCCTGTAA624510.9119576986907885No Hit
TGTAAACATCCTTGACTGGAAGCT537510.784913584447464No Hit
TATTGCACTTGTCCCGGCCTGTAT529800.7736548474265901No Hit
TATTGCACTTGTCCCGGCCTGT475800.6947998799652162No Hit
TGAGGTAGTAGATTGTATAGTT473920.6920545588758202No Hit
CATAAAGTAGAAAGCACTACT454070.6630680569478893No Hit
TTCTCACTACTGCACTTGACTAG356050.5199316882337437No Hit
TTCAAGTAATTCAGGATAGGTT347110.5068768102873606No Hit
AGCAGCATTGTACAGGGCTATGA344900.5036495977301452No Hit
CAACGGAATCCCAAAAGCAGCTGT335820.4903902809792327No Hit
CCCCCACTGCTAAATTTGACTGGCTT326110.4762109896079375No Hit
TATTGCACTTGTCCCGGCCTGTA307170.44855333990944823No Hit
TGTAAACATCCCCGACTGGAAGCT298790.43631621718118313No Hit
ACCGGGTGCTGTAGGCTT295670.43176015239452603No Hit
CGCCTGTCTGAGCGTCGCT284630.4156386923802007No Hit
ACTGGACTTGGAGTCAGAAGGC274920.40145940100890554No Hit
CCCATAAAGTAGAAAGCACTAC240380.35102142737713043No Hit
ACCGGGTGCTGTAGGCTTT236700.34564760737235534No Hit
TCGTACGACTCTTAGCGG235470.3438514664468463No Hit
CACCCGGCTGTGTGCACATGTGC218160.3185740685439503No Hit
CTTCTCACTACTGCACTTGACTAG215900.3152738421279743No Hit
ACCGGGTGCTGTAGGCTTA212540.3103673108192666No Hit
CAACGGAATCCCAAAAGCAGCT209190.30547538228231097No Hit
CATTGCACTTGTCTCGGTCTGA195990.2861997235695307No Hit
AACCGGGCGGAAACACCA195890.2860536958520096No Hit
CTCCCACTGCTTCACTTGACTAG195380.28530895449265226No Hit
CCCCCCACTGCTAAATTTGACTGGCT186570.2724439125790466No Hit
TGAGGTAGTAGTTTGTACAGTT178920.2612727921886853No Hit
CCCCCCACTGCTAAATTTGACTGG175720.2565999052280113No Hit
AACGGAATCCCAAAAGCAGCTG162050.23663791624288205No Hit
CCCATAAAGTAGAAAGCACT161640.23603920260104572No Hit
CTACGCCTGTCTGAGCGTCGCT159850.23342530645741869No Hit
CTCCCACTGCTTCACTTGACTAGCCT152390.22253163873034743No Hit
ACCCCCCACTGCTAAATTTGACTG152080.2220789528060321No Hit
TAGCAGCACGTAAATATTGGC151910.22183070568624633No Hit
CAACACCAGTCGATGGGCTGT143910.21014848828456129No Hit
AGCTCGGTCTGAGGCCCCTCAGT142270.20775363371721586No Hit
TAGCTTATCAGACTGATGTTGACA131220.19161757093113843No Hit
CAAAGAATTCTCCTTTTGGGCT130030.18987984109263778No Hit
AACATTCAACGCTGTCGGTGAGTT126690.1850025153274343No Hit
TACGCCTGTCTGAGCGTCGCT125860.18379048527200947No Hit
TGAGGTAGTAGTTTGTGCTGTT122640.17908839276783128No Hit
TGTAAACATCCTACACTCTCAGCT122340.17865030961526807No Hit
TTCAAGTAATCCAGGATAGGCTT102230.14928413562178236No Hit
TGAGGTAGTAGGTTGTGTGGTT98550.14391031561700726No Hit
TTCACAGTGGCTAAGTTCTGC95180.13898918153654743No Hit
TTCACAGTGGCTAAGTTCCG92820.13554292740305038No Hit
TTCAAGTAATCCAGGATAGGC92220.134666761097924No Hit
GACCCGCGGGCGCTCTCCAGTCCT90150.131643987345238No Hit
TGTAAACATCCCCGACTGGAAGC89580.13081162935536794No Hit
TATTGCACTTGTCCCGGCCTGTT87810.12822693875524513No Hit
CAAAGAATTCTCCTTTTGGGCTT86670.12656222277550502No Hit
AATGCACCCGGGCAAGGATTCT85190.12440101255619329No Hit
TGAGGTAGTAGGTTGTATAGT83580.12204996630410418No Hit
ACGCCTGTCTGAGCGTCGCT81460.11895417869265765No Hit
CCCCCACTGCTAAATTTGACTGGCT77430.11306926167655883No Hit
TTATAAAGCAATGAGACTGATT74780.10919952716225068No Hit
TTCACAGTGGCTAAGTTCCGC74360.1085862107486622No Hit
CAACGGAATCCCAAAAGCAGCTGA73790.10775385275879215No Hit
AGAGGTAGTAGGTTGCATAGTT73580.10744719455199792No Hit
CGCGACCTCAGATCAGACGTGGCG71420.10429299585354296No Hit
CCCTGTCTGAGCGTCGCT69680.10175211356867647No Hit
CACTAGATTGTGAGCTCCTGGA69400.1013432359596175No Hit
TATTGCACTTGTCCCGGCCTGTAG69130.10094896112231064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)