FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6497958
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5270938.111671389688883No Hit
CAACGGAATCCCAAAAGCAGCTG4156306.39631711993214No Hit
GCCTGTCTGAGCGTCGCT3097114.766281961194578No Hit
CCCCCACTGCTAAATTTGACTG2092723.2205809886736727No Hit
TTCAAGTAATCCAGGATAGGCT1875012.8855372718629453No Hit
AAGCTGCCAGTTGAAGAACTGT1726912.657619516777425No Hit
TAGCAGCACGTAAATATTGGCG1345892.0712506913710427No Hit
AACATTCAACGCTGTCGGTGAGT1218341.874958256116768No Hit
AACATTCAACGCTGTCGGTGAG983721.513890979289186No Hit
TGAGGTAGTAGGTTGTATAGTT685051.0542542749583792No Hit
TAGCTTATCAGACTGATGTTGAC657741.0122256869004078No Hit
ACCGGGTGCTGTAGGCTT648600.9981597295642723No Hit
TAGCTTATCAGACTGATGTTGACT626760.9645491706779268No Hit
TATTGCACTTGTCCCGGCCTGT587910.9047611572743314No Hit
TATTGCACTTGTCCCGGCCTGTAA583830.8984822616582009No Hit
TATTGCACTTGTCCCGGCCTGTAT541890.833938908192389No Hit
TAGCTTATCAGACTGATGTTGA541260.8329693728398982No Hit
ACCGGGTGCTGTAGGCTTT493420.7593462438507604No Hit
ACCGGGTGCTGTAGGCTTA447310.6883854897184624No Hit
CATAAAGTAGAAAGCACTACT390490.6009426345938217No Hit
TTAATGCTAATCGTGATAGGGGTT385930.5939250453757934No Hit
TGTAAACATCCTTGACTGGAAGCT368130.5665318243054203No Hit
TGAGGTAGTAGATTGTATAGTT348900.5369379118793935No Hit
TATTGCACTTGTCCCGGCCTGTA322590.4964482688253756No Hit
TTCAAGTAATTCAGGATAGGTT321790.49521711282221276No Hit
GGAATACCGGGTGCTGTAGGCTTT299760.4613141543851161No Hit
AGCAGCATTGTACAGGGCTATGA292680.450418423757125No Hit
CAACGGAATCCCAAAAGCAGCTGT271030.4171002644215306No Hit
TGAGAACTGAATTCCATGGGTT270910.41691559102105613No Hit
AACCGGGCGGAAACACCA263960.40621992324357903No Hit
TCCTGTCTGAGCGTCGCT257900.3968939165196205No Hit
CAACGGAATCCCAAAAGCAGCT256910.3953703609657065No Hit
ACTGGACTTGGAGTCAGAAGGC235320.3621445383303493No Hit
GGAATACCGGGTGCTGTAGGCTT233200.35888197492196783No Hit
TGTAAACATCCCCGACTGGAAGCT229280.3528493105064699No Hit
TCGTACGACTCTTAGCGG207330.31906946766968947No Hit
CGCCTGTCTGAGCGTCGCT207250.31894635206937316No Hit
CATTGCACTTGTCTCGGTCTGA198720.3058191511856494No Hit
CCCATAAAGTAGAAAGCACTAC189810.2921071512004233No Hit
TTCTCACTACTGCACTTGACTAG174990.26929998624183166No Hit
CGCGACCTCAGATCAGACGTGG172850.2660066439333711No Hit
CAACACCAGTCGATGGGCTGT166950.25692686841004514No Hit
ACCCCCCACTGCTAAATTTGACTG161750.24892435438948665No Hit
CTCCCACTGCTTCACTTGACTAG150730.23196518044591852No Hit
CCCATAAAGTAGAAAGCACT150570.23171894924528597No Hit
TCGTTTCCCGGCCAATGCACCA149170.22956442623975104No Hit
CCCCCACTGCTAAATTTGACTGGCTT148080.22788697618544165No Hit
CACCCGGCTGTGTGCACATGTGC142410.21916115801302502No Hit
TGAGGTAGTAGTTTGTACAGTT137470.21155876969349446No Hit
GGGAATACCGGGTGCTGTAGGCTT122270.18816680563340052No Hit
AGCTCGGTCTGAGGCCCCTCAGT120740.1858122197773516No Hit
TGAGAACTGAATTCCATAGGCTGT119700.1842117169732399No Hit
ATCCCGGACGAGCCCCCA119400.18375003347205382No Hit
GGGAATACCGGGTGCTGTAGGCTTT117190.1803489650133165No Hit
AACGGAATCCCAAAAGCAGCTG113840.1751934992500721No Hit
CGCGACCTCAGATCAGACGTGGCG108800.16743721643014622No Hit
CTACGCCTGTCTGAGCGTCGCT101990.15695700095322254No Hit
TATTGCACTTGTCCCGGCCTGTT99100.15250944989179677No Hit
CCCCCCACTGCTAAATTTGACTGG95970.14769255202942216No Hit
TAGCTTATCAGACTGATGTTGACA94170.14492245102230578No Hit
TGTAAACATCCTACACTCTCAGCT93770.14430687302072437No Hit
TGAGGTAGTAGTTTGTGCTGTT92600.1425063073660987No Hit
TTCAAGTAATCCAGGATAGGC90830.1397823747091009No Hit
TAGCAGCACGTAAATATTGGC90620.1394591962582707No Hit
TTCACAGTGGCTAAGTTCCG88540.1362581906500473No Hit
CTTCTCACTACTGCACTTGACTAG88170.13568878099858447No Hit
GTGCGGTAACGCGACCGATCCCGGA87640.1348731401464891No Hit
TTCAAGTAATCCAGGATAGGCTT86640.13333419514253556No Hit
CACTAGATTGTGAGCTCCTGGA85590.13171830288838432No Hit
TGAGGTAGTAGGTTGTGTGGTT83390.1283326238796865No Hit
CCCCCCACTGCTAAATTTGACTGT83390.1283326238796865No Hit
AACATTCAACGCTGTCGGTGAGTT80820.1243775352195259No Hit
TGATGTAGTAGGTTGTATAGTT80430.12377734666798401No Hit
CAAAGAATTCTCCTTTTGGGCT78600.120961077310749No Hit
TACGCCTGTCTGAGCGTCGCT75860.11674436799991629No Hit
CAACGGAATCCCAAAAGCAGCTGA75320.11591333769778137No Hit
CCCCCCACTGCTAAATTTGACTGGCT73740.11348180459153476No Hit
AATGCACCCGGGCAAGGATTCT73010.11235837473864867No Hit
TTCACAGTGGCTAAGTTCTGC72750.11195824903762074No Hit
CCACCCTGAACGCGCCCGA72000.11080404028465558No Hit
ATACCGGGTGCTGTAGGCTTA71580.1101576833829951No Hit
ATACCGGGTGCTGTAGGCTT69600.10711057227516706No Hit
AGCAGCATTGTACAGGGCTATGT69280.10661810987390191No Hit
CACTAGATTGTGAGCTCCTGGAA67130.10330937811540179No Hit
CCCCCCACTGCTAAATTTGACTGTA65960.10150881246077613No Hit
TTCACAGTGGCTAAGTTCTG65580.1009240133592738No Hit
TATTGCACTTGTCCCGGCCTGTAG65300.10049310875816679No Hit
CGCGACCTCAGATCAGACGTGGC65260.10043155095800864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)