FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6938023
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5574348.034478986304888No Hit
CAACGGAATCCCAAAAGCAGCTG4351166.27146955263769No Hit
GCCTGTCTGAGCGTCGCT2698933.890056288369179No Hit
AAGCTGCCAGTTGAAGAACTGT2427273.498503824504473No Hit
CCCCCACTGCTAAATTTGACTG2407053.4693600756296137No Hit
TTCAAGTAATCCAGGATAGGCT1743312.5126898541558598No Hit
TAGCAGCACGTAAATATTGGCG1654592.3848148096366937No Hit
AACATTCAACGCTGTCGGTGAGT1531922.208006517130312No Hit
AACATTCAACGCTGTCGGTGAG1083611.561842617125945No Hit
TCCTGTCTGAGCGTCGCT1054441.5197989398420848No Hit
ACCGGGTGCTGTAGGCTT992661.430753400500402No Hit
TGAGGTAGTAGGTTGTATAGTT831851.1989726756454973No Hit
TAGCTTATCAGACTGATGTTGAC749471.080235680971366No Hit
ACCGGGTGCTGTAGGCTTT722231.040973775958944No Hit
TAGCTTATCAGACTGATGTTGACT691040.996018606453164No Hit
TATTGCACTTGTCCCGGCCTGT684200.9861598902165645No Hit
TAGCTTATCAGACTGATGTTGA620430.8942460986364559No Hit
CGCCTGTCTGAGCGTCGCT590180.850645781946817No Hit
ACCGGGTGCTGTAGGCTTA551860.7954139096973302No Hit
TATTGCACTTGTCCCGGCCTGTAA527290.7600003632158614No Hit
CATAAAGTAGAAAGCACTACT493470.7112544884904533No Hit
TATTGCACTTGTCCCGGCCTGTAT471300.679300140688493No Hit
TTAATGCTAATCGTGATAGGGGTT410120.5911194010166874No Hit
TGAGGTAGTAGATTGTATAGTT406280.5855846831294736No Hit
TCGTACGACTCTTAGCGG403570.5816786712871952No Hit
TGTAAACATCCTTGACTGGAAGCT367240.5293150512761344No Hit
TATTGCACTTGTCCCGGCCTGTA315120.4541927866194736No Hit
AGCAGCATTGTACAGGGCTATGA309550.44616456301744745No Hit
ACTGGACTTGGAGTCAGAAGGC303570.4375453929743387No Hit
CAACGGAATCCCAAAAGCAGCTGT288540.4158821612439163No Hit
CAACACCAGTCGATGGGCTGT265130.3821405607908766No Hit
TTCAAGTAATTCAGGATAGGTT264300.3809442545808799No Hit
TGTAAACATCCCCGACTGGAAGCT225990.3257267956592245No Hit
CATTGCACTTGTCTCGGTCTGA216130.3115152544175769No Hit
AACCGGGCGGAAACACCA212020.30559137667891845No Hit
CCCATAAAGTAGAAAGCACT202920.29247524835244854No Hit
ATCCCGGACGAGCCCCCA202890.29243200836895467No Hit
CAACGGAATCCCAAAAGCAGCT190690.27484774841478615No Hit
CCCATAAAGTAGAAAGCACTAC186460.26875091074215235No Hit
TACGCCTGTCTGAGCGTCGCT165430.23843968231295862No Hit
TGAGGTAGTAGTTTGTACAGTT164250.2367389096288669No Hit
TGAGAACTGAATTCCATGGGTT159480.22986375225334363No Hit
TGAGAACTGAATTCCATAGGCTGT154130.22215262186360582No Hit
TTCTCACTACTGCACTTGACTAG143290.2065285744944922No Hit
CACCCGGCTGTGTGCACATGTGC138370.19943721720149962No Hit
ACGCCTGTCTGAGCGTCGCT135010.19459433905018764No Hit
AACGGAATCCCAAAAGCAGCTG133860.1929368063495898No Hit
CTACGCCTGTCTGAGCGTCGCT123820.17846582520697898No Hit
AGCTCGGTCTGAGGCCCCTCAGT123810.1784514118791477No Hit
TCGTTTCCCGGCCAATGCACCA115040.16581092337111017No Hit
CCCTGTCTGAGCGTCGCT112260.16180401823401278No Hit
ACCCCCCACTGCTAAATTTGACTG111430.16060771202401608No Hit
AACATTCAACGCTGTCGGTGAGTT108130.15585131383969178No Hit
TGAGGTAGTAGTTTGTGCTGTT105480.15203178196440112No Hit
TTCACAGTGGCTAAGTTCCG104720.1509363690492234No Hit
TGAGGTAGTAGGTTGTGTGGTT104220.1502157026576591No Hit
CGCGACCTCAGATCAGACGTGG103490.14916352972597524No Hit
TAGCAGCACGTAAATATTGGC101860.1468141572894757No Hit
TATTGCACTTGTCCCGGCCTGTT99290.14310993203683528No Hit
TGTAAACATCCTACACTCTCAGCT93770.1351537750739656No Hit
TTCAAGTAATCCAGGATAGGCTT86970.12535271214869137No Hit
CCCCCCACTGCTAAATTTGACTGG85930.12385372605423764No Hit
TAGCTTATCAGACTGATGTTGACA85550.12330601959664879No Hit
TTCAAGTAATCCAGGATAGGC85370.12304657969568564No Hit
CTCCCACTGCTTCACTTGACTAG83470.12030804740774137No Hit
TTCACAGTGGCTAAGTTCTG83230.1199621275397905No Hit
AATGCACCCGGGCAAGGATTCT82480.11888112795244409No Hit
CACCCGTAGAACCGACCTTGCG82270.11857844806798709No Hit
CCCCCACTGCTAAATTTGACTGGCTT81220.11706504864570211No Hit
TTCACAGTGGCTAAGTTCTGC80870.1165605821716071No Hit
TGAGGTAGTAGGTTGTATAGT79460.11452830294739581No Hit
ACCACAGGGTAGAACCACGGAC79180.11412472976811983No Hit
TTATAAAGCAATGAGACTGATT78660.11337523672089297No Hit
CTGGACGCGAGCCGGGCCCT78470.11310138349209854No Hit
CACTAGATTGTGAGCTCCTGGA77630.11189066395427055No Hit
CTTCTCACTACTGCACTTGACTAG73490.10592354623211828No Hit
CAAAGAATTCTCCTTTTGGGCT72920.105101986545735No Hit
AGCAGCATTGTACAGGGCTATGT71740.10340121386164329No Hit
AATGACACGATCACTCCCGTTG70760.10198870773417731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)