FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6943364
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5750368.28180691664732No Hit
GCCTGTCTGAGCGTCGCT4627616.664795335517482No Hit
CAACGGAATCCCAAAAGCAGCTG4209306.062335202360123No Hit
CCCCCACTGCTAAATTTGACTG2385583.4357697508009086No Hit
AAGCTGCCAGTTGAAGAACTGT1930232.779963717875082No Hit
TTCAAGTAATCCAGGATAGGCT1874362.6994983987588728No Hit
TAGCAGCACGTAAATATTGGCG1514052.180571261999227No Hit
AACATTCAACGCTGTCGGTGAG1010941.4559801272121122No Hit
AACATTCAACGCTGTCGGTGAGT977761.408193492376318No Hit
ACCGGGTGCTGTAGGCTT841391.2117901351563882No Hit
TGAGGTAGTAGGTTGTATAGTT699211.007019076055929No Hit
ACCGGGTGCTGTAGGCTTT650090.9362752694515223No Hit
CATAAAGTAGAAAGCACTACT601210.865877116625313No Hit
TAGCTTATCAGACTGATGTTGA584570.8419117880036248No Hit
ACCGGGTGCTGTAGGCTTA580680.8363093163486749No Hit
TAGCTTATCAGACTGATGTTGAC574670.827653569652981No Hit
TAGCTTATCAGACTGATGTTGACT555610.8002028987678019No Hit
TATTGCACTTGTCCCGGCCTGT531660.7657095321518503No Hit
TATTGCACTTGTCCCGGCCTGTAA481170.6929926185635666No Hit
TATTGCACTTGTCCCGGCCTGTAT448070.6453212016538381No Hit
TTAATGCTAATCGTGATAGGGGTT419440.6040875863630367No Hit
TATTGCACTTGTCCCGGCCTGTA372990.5371891780410764No Hit
TGTAAACATCCTTGACTGGAAGCT356880.513987168179574No Hit
TGAGGTAGTAGATTGTATAGTT349160.5028686383142235No Hit
ATCCCGGACGAGCCCCCA299200.43091504348612575No Hit
CGCCTGTCTGAGCGTCGCT294370.42395876119990256No Hit
TCGTACGACTCTTAGCGG272520.3924898651431784No Hit
CAACACCAGTCGATGGGCTGT272210.3920433956796734No Hit
TTCAAGTAATTCAGGATAGGTT258070.3716786272475417No Hit
TCCTGTCTGAGCGTCGCT251180.3617554833651239No Hit
AACCGGGCGGAAACACCA239140.3444151854922196No Hit
CAACGGAATCCCAAAAGCAGCTGT236020.33992168637565306No Hit
AGCAGCATTGTACAGGGCTATGA231990.33411758335008795No Hit
TGAGAACTGAATTCCATGGGTT231880.3339591587017474No Hit
CATTGCACTTGTCTCGGTCTGA196810.2834505003626484No Hit
CCCATAAAGTAGAAAGCACTAC191680.2760621508536784No Hit
TGTAAACATCCCCGACTGGAAGCT184420.2656061240632062No Hit
TTCACAGTGGCTAAGTTCCG182400.26269687143004455No Hit
GGAATACCGGGTGCTGTAGGCTTT179750.25888027762911464No Hit
ACTGGACTTGGAGTCAGAAGGC178550.25715200873812755No Hit
TTCTCACTACTGCACTTGACTAG171990.24770413880073117No Hit
CTACGCCTGTCTGAGCGTCGCT167480.24120872821877118No Hit
CCCATAAAGTAGAAAGCACT164850.23742093889935773No Hit
GGAATACCGGGTGCTGTAGGCTT160650.23137199778090276No Hit
ACCCCCCACTGCTAAATTTGACTG146540.21105043607104568No Hit
TGAGGTAGTAGTTTGTACAGTT136950.19723868718390683No Hit
TGAGAACTGAATTCCATAGGCTGT125160.18025844532995822No Hit
TGATGTAGTAGGTTGTATAGTT120050.17289890030250465No Hit
ACCTGGTGCTGTAGGCTT116230.1673972443328623No Hit
ACGCCTGTCTGAGCGTCGCT111690.1608586270286276No Hit
CTCCCACTGCTTCACTTGACTAG111660.16081542030635293No Hit
CCCCCACTGCTAAATTTGACTGGCTT109450.15763252509878495No Hit
TAGCAGCACGTAAATATTGGC108590.15639393239357752No Hit
TTCACAGTGGCTAAGTTCTG108210.15584664724476494No Hit
TTCAAGTAATCCAGGATAGGC107370.15463685902107394No Hit
TACGCCTGTCTGAGCGTCGCT105370.15175641086942873No Hit
CAAAGAATTCTCCTTTTGGGCT104930.15112271227606677No Hit
AACGGAATCCCAAAAGCAGCTG104760.1508778741831769No Hit
GGGAATACCGGGTGCTGTAGGCTTT101790.14660040867798377No Hit
GGGAATACCGGGTGCTGTAGGCTT101560.14626915714054456No Hit
CACCCGGCTGTGTGCACATGTGC101050.14553464286187504No Hit
TATTGCACTTGTCCCGGCCTGTT99430.14320147985904239No Hit
CAACGGAATCCCAAAAGCAGCT98690.14213571404293365No Hit
AGCTCGGTCTGAGGCCCCTCAGT97690.14069548996711104No Hit
ATACCGGGTGCTGTAGGCTT92650.13343676062496507No Hit
ATACCGGGTGCTGTAGGCTTA92650.13343676062496507No Hit
CTTCTCACTACTGCACTTGACTAG91680.13203974327141715No Hit
CGCGACCTCAGATCAGACGTGG88180.126998959006038No Hit
TGTAAACATCCTACACTCTCAGCT87700.12630765144964315No Hit
ACCTGGTGCTGTAGGCTTT87660.12625004248661023No Hit
TGAGGTAGTAGTTTGTGCTGTT84980.12239024196340563No Hit
CCCCCCACTGCTAAATTTGACTGG82000.11809837421745426No Hit
ACCTGGTGCTGTAGGCTTA81840.11786793836532264No Hit
ATACCGGGTGCTGTAGGCTTT79220.11409455128666739No Hit
AGCTACATTGTCTGCTGGGTTT78810.1135040594155801No Hit
CCCATAAAGTAGAAAGCACTA76780.11058040454166021No Hit
AACATTCAACGCTGTCGGTGAGTT76220.10977387905919955No Hit
GACCCGCGGGCGCTCTCCAGTCCT74710.10759914070470741No Hit
TGAGGTAGTAGGTTGTGTGGTT73190.10541000010945703No Hit
TAGCTTATCAGACTGATGTTGACA72110.10385455810756862No Hit
TGAGGTAGTAGGTTGTATAGT72040.10375374242226103No Hit
TCGTTTCCCGGCCAATGCACCA71730.10330727295875602No Hit
CACTAGATTGTGAGCTCCTGGA71460.10291841245828391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)