FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4781960
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG48631810.16984667374884No Hit
CAACGGAATCCCAAAAGCAGCTG3168646.626236940501384No Hit
CCCCCACTGCTAAATTTGACTG2128704.451521970070849No Hit
AAGCTGCCAGTTGAAGAACTGT1343582.8096847317836198No Hit
TTCAAGTAATCCAGGATAGGCT1229022.5701176923269955No Hit
TAGCAGCACGTAAATATTGGCG1107722.3164560138520605No Hit
AACATTCAACGCTGTCGGTGAGT1068272.2339584605475578No Hit
ACCGGGTGCTGTAGGCTT788081.6480271687759829No Hit
AACATTCAACGCTGTCGGTGAG766221.602313695639445No Hit
TGAGGTAGTAGGTTGTATAGTT527541.103187814201708No Hit
ACCGGGTGCTGTAGGCTTT510781.0681394240018738No Hit
TAGCTTATCAGACTGATGTTGAC502481.0507825243205715No Hit
TAGCTTATCAGACTGATGTTGA432250.9039180587039625No Hit
TAGCTTATCAGACTGATGTTGACT428020.8950723134446963No Hit
ACCGGGTGCTGTAGGCTTA387210.8097307380237392No Hit
CGCCTGTCTGAGCGTCGCT371440.776752628629265No Hit
TATTGCACTTGTCCCGGCCTGT338620.7081196831424772No Hit
CATAAAGTAGAAAGCACTACT336060.7027662297467984No Hit
TCGTACGACTCTTAGCGG295330.6175919497444562No Hit
TATTGCACTTGTCCCGGCCTGTAA286980.6001304904265197No Hit
TGAGGTAGTAGATTGTATAGTT255660.5346343340387624No Hit
TGTAAACATCCTTGACTGGAAGCT255630.5345715982567817No Hit
TATTGCACTTGTCCCGGCCTGTAT252550.5281307246401057No Hit
TATTGCACTTGTCCCGGCCTGTA221020.46219541777848416No Hit
AGCAGCATTGTACAGGGCTATGA214360.44826807417878867No Hit
ACTGGACTTGGAGTCAGAAGGC203620.42580866422973007No Hit
CAACGGAATCCCAAAAGCAGCTGT178090.3724205137642306No Hit
CAACACCAGTCGATGGGCTGT172920.36160904733623866No Hit
TTCAAGTAATTCAGGATAGGTT161440.3376021547649917No Hit
TGTAAACATCCCCGACTGGAAGCT161250.33720482814578123No Hit
TTCTCACTACTGCACTTGACTAG160200.33500907577645983No Hit
CCCCCACTGCTAAATTTGACTGGCTT155470.3251177341508503No Hit
CAACGGAATCCCAAAAGCAGCT142620.29824590753582214No Hit
CATTGCACTTGTCTCGGTCTGA127630.26689892847284374No Hit
CCCATAAAGTAGAAAGCACT125900.26328116504529525No Hit
CCCATAAAGTAGAAAGCACTAC119810.2505458013032313No Hit
CTCCCACTGCTTCACTTGACTAG104620.21878058369371556No Hit
TGAGGTAGTAGTTTGTACAGTT103220.21585291386795372No Hit
TTCACAGTGGCTAAGTTCCG102710.21478640557428333No Hit
TACGCCTGTCTGAGCGTCGCT98110.2051669190039231No Hit
ACCCCCCACTGCTAAATTTGACTG96090.2009427096838953No Hit
ATCCCGGACGAGCCCCCA94210.1970112673464437No Hit
AACGGAATCCCAAAAGCAGCTG92070.1925361148984935No Hit
AACATTCAACGCTGTCGGTGAGTT89330.18680624681093108No Hit
CCCCCCACTGCTAAATTTGACTGG86460.18080452366811933No Hit
CTACGCCTGTCTGAGCGTCGCT84030.1757229253276899No Hit
GCCTGTCTGAGCGTCGCT82240.17197969033618013No Hit
TGTCAGTTTGTCAAATACCCCAA81630.17070406276924105No Hit
CTTCTCACTACTGCACTTGACTAG80960.16930296363834074No Hit
ACGCCTGTCTGAGCGTCGCT80270.16786004065278673No Hit
CCCCCCACTGCTAAATTTGACTGGCT77140.16131460739947637No Hit
TGAGGTAGTAGTTTGTGCTGTT71500.14952028038712162No Hit
TAGCAGCACGTAAATATTGGC70940.14834921245681687No Hit
TTCACAGTGGCTAAGTTCTG68730.14372767651757856No Hit
CACCCGGCTGTGTGCACATGTGC67380.14090456632845108No Hit
ACTGGACTTGGAGTCAGAAGG65610.13720315519159507No Hit
CGCGACCTCAGATCAGACGTGG65410.13678491664505768No Hit
TGAGGTAGTAGGTTGTGTGGTT64770.135446553296138No Hit
TCGTTTCCCGGCCAATGCACCA64200.13425457343850639No Hit
CTGGACGCGAGCCGGGCCCT62540.13078319350224593No Hit
TAGCTTATCAGACTGATGTTGACA61490.12858744113292458No Hit
AGCTCGGTCTGAGGCCCCTCAGT61070.12770914018519602No Hit
TTCAAGTAATCCAGGATAGGC59990.12545065203389405No Hit
ACTGGACTTGGAGTCAGAAGGCT59240.1238822574843788No Hit
AATGCACCCGGGCAAGGATTCT58820.12300395653665024No Hit
TATTGCACTTGTCCCGGCCTGTT58800.12296213268199649No Hit
CAAAGAATTCTCCTTTTGGGCT56860.1189052187805837No Hit
ATACCGGGTGCTGTAGGCTT54870.11474374524253653No Hit
CTCCCACTGCTTCACTTGACTAGCCT54830.11466009753322906No Hit
TGTAAACATCCTACACTCTCAGCT53870.11265255250984953No Hit
AGCAGCATTGTACAGGGCTATG53740.1123806974546002No Hit
TGAGGTAGTAGGTTGTATAGT53240.1113351010882567No Hit
TTATAAAGCAATGAGACTGATT53140.11112598181498799No Hit
TTCAAGTAATCCAGGATAGGCTT51290.10725727525951702No Hit
TTCACAGTGGCTAAGTTCTGC51240.10715271562288266No Hit
GCGTTCGCGCTTTCCCCTG50690.1060025596199048No Hit
ACCACAGGGTAGAACCACGGAC50400.10539611372742558No Hit
CGCGACCTCAGATCAGACGTGGCG49900.10435051736108207No Hit
ACTGGACTTGGAGTCAGAAGGCA49470.10345130448602664No Hit
GACCCGCGGGCGCTCTCCAGTCCT48850.1021547649917607No Hit
TGTCAGTTTGTCAAATACCCCA48530.10148558331730086No Hit
GGAATACCGGGTGCTGTAGGCTTT48130.10064910622422606No Hit
AACCGGGCGGAAACACCA48010.1003981630963036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)