FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7934056
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6697468.441407522205541No Hit
CAACGGAATCCCAAAAGCAGCTG4536295.7174917847819575No Hit
TTCAAGTAATCCAGGATAGGCT3552524.477558514837808No Hit
AAGCTGCCAGTTGAAGAACTGT3408904.296541390683403No Hit
TAGCAGCACGTAAATATTGGCG2748373.4640163870786895No Hit
TAGCTTATCAGACTGATGTTGAC2420753.0510876152121944No Hit
AACATTCAACGCTGTCGGTGAGT2088802.632701357288126No Hit
TAGCTTATCAGACTGATGTTGACT1848112.3293382350716962No Hit
CCCCCACTGCTAAATTTGACTG1695812.1373809310143512No Hit
GCCTGTCTGAGCGTCGCT1598672.0149467056950443No Hit
TAGCTTATCAGACTGATGTTGA1442441.8180360713360229No Hit
TGTAAACATCCTTGACTGGAAGCT1335781.6836029390263945No Hit
TGAGGTAGTAGGTTGTATAGTT1168541.47281541748634No Hit
AACATTCAACGCTGTCGGTGAG1106801.3949989765638156No Hit
TATTGCACTTGTCCCGGCCTGTAT1086251.3690979746046663No Hit
TATTGCACTTGTCCCGGCCTGTAA1064051.3411173301524466No Hit
TGAGGTAGTAGATTGTATAGTT990221.2480627815079703No Hit
TTCAAGTAATTCAGGATAGGTT794061.0008247988166457No Hit
TATTGCACTTGTCCCGGCCTGT707330.891511227044528No Hit
AACCGGGCGGAAACACCA704110.8874527732095664No Hit
AGCAGCATTGTACAGGGCTATGA664360.8373522949674164No Hit
CATAAAGTAGAAAGCACTACT662010.8343903799015282No Hit
TGTAAACATCCCCGACTGGAAGCT538490.67870708248089No Hit
TGTAAACATCCTACACTCTCAGCT505700.6373789143913278No Hit
TATTGCACTTGTCCCGGCCTGTA455180.5737040424216819No Hit
ACTGGACTTGGAGTCAGAAGGC417600.5263386091552669No Hit
TGAGGTAGTAGTTTGTACAGTT363370.45798769254968713No Hit
CTTCTCACTACTGCACTTGACTAG344720.4344814304310431No Hit
CCCATAAAGTAGAAAGCACTAC344490.434191540871403No Hit
TTCTCACTACTGCACTTGACTAG340140.4287088470260356No Hit
CAACGGAATCCCAAAAGCAGCTGT339740.4282046912701397No Hit
CGCCTGTCTGAGCGTCGCT312410.39375824924855585No Hit
CATTGCACTTGTCTCGGTCTGA306610.3864479907880661No Hit
CTCCCACTGCTTCACTTGACTAG269610.33981358336769996No Hit
TAGCTTATCAGACTGATGTTGACA264700.3336250714640784No Hit
TTCAAGTAATCCAGGATAGGCTT250770.3160678472650055No Hit
TTATAAAGCAATGAGACTGATT235170.2964057727850673No Hit
TGTAAACATCCTACACTCAGCT231910.29229690337451614No Hit
TCGTACGACTCTTAGCGG230240.290192053093651No Hit
CACCCGGCTGTGTGCACATGTGC216270.2725844133189884No Hit
CCCATAAAGTAGAAAGCACT210310.2650724925561403No Hit
CAAAGAATTCTCCTTTTGGGCT205150.2585688833050838No Hit
AACATTCAACGCTGTCGGTGAGTT195520.24643133348189122No Hit
TGTCAGTTTGTCAAATACCCCA192290.24236027575303223No Hit
AGCTCGGTCTGAGGCCCCTCAGT185160.23337369940418873No Hit
CCCCCCACTGCTAAATTTGACTGGCT184000.23191164771209075No Hit
TGTCAGTTTGTCAAATACCCCAA181340.2285590119353834No Hit
CCCCCCACTGCTAAATTTGACTGG181150.22831953795133283No Hit
TGAGGTAGTAGTTTGTGCTGTT174780.22029085753869143No Hit
CAAAGAATTCTCCTTTTGGGCTT174360.2197614939950008No Hit
TTCACAGTGGCTAAGTTCCG162680.205040145922842No Hit
CTACGCCTGTCTGAGCGTCGCT160900.20279665280910544No Hit
AGCAGCATTGTACAGGGCTATGT151960.1915287716648332No Hit
TGTAAACATCCCCGACTGGAAGC150920.190217966699504No Hit
TACGCCTGTCTGAGCGTCGCT149680.18865508385622687No Hit
TGTCAGTTTGTCAAATACCCC148690.18740729836038464No Hit
TTCAAGTAATCCAGGATAGGC147730.1861973245462346No Hit
CAACGGAATCCCAAAAGCAGCT143090.18034911777784277No Hit
TAGCAGCACGTAAATATTGGC139170.17540839137006342No Hit
TATTGCACTTGTCCCGGCCTGTT132570.16708982139778192No Hit
TGATGTAGTAGGTTGTATAGTT131630.16590505537142666No Hit
TTCACAGTGGCTAAGTTCTGC122920.15492706378679455No Hit
TTCACAGTGGCTAAGTTCCGC121860.15359105103367054No Hit
CCCCCACTGCTAAATTTGACTGGCTT121640.15331376536792782No Hit
TGTAAACATCCTTGACTGGAAGC120850.15231805775003351No Hit
CAAAGTGCTGTTCGTGCAGGTAG118460.14930572710855583No Hit
TAGCAGCACGTAAATATTGGCGT117880.14857470126250685No Hit
CAACACCAGTCGATGGGCTGT114640.14449103963975046No Hit
TATTGCACTTGTCCCGGCCTGTAG112800.14217192316262955No Hit
TGATGTAGTAGATTGTATAGTT111870.1409997610301717No Hit
AACGGAATCCCAAAAGCAGCTG108140.1362985086064429No Hit
GGAATACCGGGTGCTGTAGGCTTT103900.13095445759394692No Hit
ACCCCCCACTGCTAAATTTGACTG103420.1303494706868719No Hit
GCGTTCGCGCTTTCCCCTG102790.12955542537133594No Hit
TGAGGTAGTAGGTTGTATAGT102420.12908908129713226No Hit
ATCACATTGCCAGGGATTTCCA102200.12881179563138953No Hit
CACTAGATTGTGAGCTCCTGGA101420.12782869190739263No Hit
TATTGCACTTGTCCCGGCCTGTTT101240.1276018218172395No Hit
TCCTGTCTGAGCGTCGCT99610.12554738711196392No Hit
ACGCCTGTCTGAGCGTCGCT96070.12108560867228566No Hit
CACTAGATTGTGAGCTCCTGGAA95970.12095956973331169No Hit
CTCCCACTGCTTCACTTGACTAGCCT95870.12083353079433773No Hit
ACCACAGGGTAGAACCACGGAC93800.11822452475757671No Hit
ATCACATTGCCAGGGATTTCC92260.11628352509737767No Hit
AGAGGTAGTAGGTTGCATAGTT91350.11513657075271463No Hit
AGCAGCATTGTACAGGGCTATG90880.11454418773953699No Hit
TATTGCACTTGTCCCGGCCTGTAAT90140.11361149959112969No Hit
CAAAGTGCTGTTCGTGCAGGT89410.11269141533661975No Hit
TTCACAGTGGCTAAGTTCTG88460.11149404541636711No Hit
TGAGGTAGTAAGTTGTATTGTT88160.11111592859944523No Hit
TAGCAGCACATAATGGTTTGT87670.1104983377984728No Hit
CAGTGCAATAGTATTGTCAAAGC86500.10902368221247746No Hit
TTCAAGTAATTCAGGATAGGTTA85590.1078767278678144No Hit
TAGTGGGTTATCAGAACT81590.1028351703088559No Hit
TTCAAGTAATCCAGGATAGGCTA80910.10197810552383295No Hit
TGAGGTAGTAGATTGTATAGT80020.1008563589669647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)