FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11154354
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8709897.808511366951416No Hit
CAACGGAATCCCAAAAGCAGCTG8703617.8028812784675825No Hit
GCCTGTCTGAGCGTCGCT5587375.009138135655368No Hit
TTCAAGTAATCCAGGATAGGCT4721454.2328314127380215No Hit
TAGCAGCACGTAAATATTGGCG3982643.5704801909639947No Hit
CCCCCACTGCTAAATTTGACTG3093402.773266833740439No Hit
AAGCTGCCAGTTGAAGAACTGT3063832.746757006277549No Hit
AACATTCAACGCTGTCGGTGAGT2707782.427554298527732No Hit
TAGCTTATCAGACTGATGTTGAC2361122.1167698281765133No Hit
AACATTCAACGCTGTCGGTGAG2062191.8487758233242373No Hit
TAGCTTATCAGACTGATGTTGACT1703691.5273766638570017No Hit
TAGCTTATCAGACTGATGTTGA1588861.4244303166279284No Hit
AACCGGGCGGAAACACCA1476371.323581804916717No Hit
TGAGGTAGTAGGTTGTATAGTT1406461.2609067275433432No Hit
TTCAAGTAATTCAGGATAGGTT1325111.18797556541598No Hit
TGTAAACATCCTTGACTGGAAGCT1197051.0731683789128441No Hit
TGAGGTAGTAGATTGTATAGTT1160191.040122986952001No Hit
TATTGCACTTGTCCCGGCCTGTAA1157241.0374782797820474No Hit
TCCTGTCTGAGCGTCGCT1130531.0135324735076545No Hit
TATTGCACTTGTCCCGGCCTGTAT1129171.0123132186767607No Hit
CATAAAGTAGAAAGCACTACT1012930.9081027910715402No Hit
TATTGCACTTGTCCCGGCCTGT897980.805048862533859No Hit
CAACGGAATCCCAAAAGCAGCT657910.5898234895539446No Hit
AGCAGCATTGTACAGGGCTATGA605180.5425504695296562No Hit
TATTGCACTTGTCCCGGCCTGTA563780.5054349180597998No Hit
TGTAAACATCCCCGACTGGAAGCT533090.47792099838323227No Hit
CAACGGAATCCCAAAAGCAGCTGT519970.46615877530872696No Hit
CCCATAAAGTAGAAAGCACTAC500550.44874853353228705No Hit
CATTGCACTTGTCTCGGTCTGA494980.4437549677910527No Hit
TTCTCACTACTGCACTTGACTAG477320.4279225852075342No Hit
TGAGGTAGTAGTTTGTACAGTT456240.4090241353286797No Hit
TGTAAACATCCTACACTCTCAGCT415870.37283199009104423No Hit
TGAGAACTGAATTCCATGGGTT405120.3631944978615525No Hit
ACTGGACTTGGAGTCAGAAGGC397630.35647963118258574No Hit
CCCATAAAGTAGAAAGCACT392490.3518715651305311No Hit
CACCCGGCTGTGTGCACATGTGC388840.34859930032702924No Hit
TTAATGCTAATCGTGATAGGGGTT380990.3415616897222376No Hit
TGTCAGTTTGTCAAATACCCCAA347030.3111161793860944No Hit
CTCCCACTGCTTCACTTGACTAG334150.2995691189288057No Hit
TCGTACGACTCTTAGCGG321610.28832687217924047No Hit
TCGTTTCCCGGCCAATGCACCA316870.2840774104892134No Hit
TGTCAGTTTGTCAAATACCCCA307940.2760715681069473No Hit
TAGCAGCACGTAAATATTGGC302330.2710421419295102No Hit
TAGCTTATCAGACTGATGTTGACA291620.26144051013622127No Hit
CTTCTCACTACTGCACTTGACTAG288630.25875994253006496No Hit
TGAGAACTGAATTCCATAGGCTGT266540.23895601663709076No Hit
CCCCCACTGCTAAATTTGACTGGCTT246240.2207568452641901No Hit
TTCAAGTAATCCAGGATAGGCTT237740.21313650257110361No Hit
AACGGAATCCCAAAAGCAGCTG232890.20878842468151898No Hit
CAAAGAATTCTCCTTTTGGGCT232770.2086808433729107No Hit
CGCCTGTCTGAGCGTCGCT222980.19990400161228522No Hit
TTCAAGTAATCCAGGATAGGC218840.19619244646529957No Hit
TGAGGTAGTAGTTTGTGCTGTT217310.194820784780544No Hit
TTATAAAGCAATGAGACTGATT208010.1864832333634023No Hit
TAGTGGGTTATCAGAACT201660.18079038911621417No Hit
TTCACAGTGGCTAAGTTCTGC196610.17626300904561573No Hit
GGAATACCGGGTGCTGTAGGCTTT193820.17376174362047322No Hit
TTCACAGTGGCTAAGTTCCGC184040.16499386696889842No Hit
CCCCCCACTGCTAAATTTGACTGG182440.16355944952078802No Hit
AACATTCAACGCTGTCGGTGAGTT179270.1607175099517193No Hit
CCCCCCACTGCTAAATTTGACTGGCT178980.1604575217892493No Hit
CAACACCAGTCGATGGGCTGT175160.15703285013188573No Hit
TTCACAGTGGCTAAGTTCCG165750.14859668251518643No Hit
TGTAAACATCCCCGACTGGAAGC163900.1469381373408088No Hit
TGTAAACATCCTACACTCAGCT163310.14640919590681808No Hit
AGCTCGGTCTGAGGCCCCTCAGT162540.14571888250991497No Hit
CACTAGATTGTGAGCTCCTGGA161830.14508235976731598No Hit
TATTGCACTTGTCCCGGCCTGTT160800.1441589535350949No Hit
CAAAGAATTCTCCTTTTGGGCTT160390.14379138406401662No Hit
ATCACATTGCCAGGGATTTCC160250.14366587253730695No Hit
TGATGTAGTAGGTTGTATAGTT158440.14204318779913208No Hit
CTACGCCTGTCTGAGCGTCGCT152260.13650275040580565No Hit
CTCCCACTGCTTCACTTGACTAGCCT144750.1297699535087375No Hit
CCCATAAAGTAGAAAGCACTA138740.1243819229692728No Hit
CACTAGATTGTGAGCTCCTGGAA134670.12073312358564198No Hit
CAACGGAATCCCAAAAGCAGCTGA133700.11986350800772506No Hit
CCCTGTCTGAGCGTCGCT132850.11910147373841641No Hit
ACCCCCCACTGCTAAATTTGACTG132540.11882355535784501No Hit
TGATGTAGTAGATTGTATAGTT131380.11778360270796498No Hit
TGTCAGTTTGTCAAATACCCC131020.11746085878214013No Hit
TATTGCACTTGTCCCGGCCTGTAG130580.11706639398390978No Hit
TGAGGTAGTAGGTTGTATAGT129490.11608919709738456No Hit
AGCAGCATTGTACAGGGCTATGT128570.11526440706472109No Hit
TTCACAGTGGCTAAGTTCTG126170.1131127808925555No Hit
AATGCACCCGGGCAAGGATTCT126040.11299623447489653No Hit
TAGCAGCACGTAAATATTGGCGT124890.11196524693406719No Hit
GGAATACCGGGTGCTGTAGGCTT122710.11001085316101676No Hit
TGAGGTAGTAGGTTGTGTGGTT120580.1081012849332198No Hit
CACCCGTAGAACCGACCTTGCG120380.10792198275220599No Hit
TGTAAACATCCTTGACTGGAAGC118270.10603034474251041No Hit
AGAGGTAGTAGGTTGCATAGTT118030.10581518212529385No Hit
CAAAGTGCTGTTCGTGCAGGTAG116800.10471247371205898No Hit
CAAAGTGCTGTTCGTGCAGGT113050.10135055781805025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)