FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6777021
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG68398910.092767899051811No Hit
GCCTGTCTGAGCGTCGCT6228299.190306478318423No Hit
CCCCCCACTGCTAAATTTGACTG5138367.582033462785493No Hit
TCCTGTCTGAGCGTCGCT1994052.942369516045472No Hit
AACATTCAACGCTGTCGGTGAGT1906162.8126812651163395No Hit
TTCAAGTAATCCAGGATAGGCT1893452.793926712046488No Hit
AAGCTGCCAGTTGAAGAACTGT1869832.7590736401731677No Hit
TAGCAGCACGTAAATATTGGCG1731622.5551344757526944No Hit
CCCCCACTGCTAAATTTGACTG1335431.9705265779757803No Hit
AACATTCAACGCTGTCGGTGAG1189151.7546795265943544No Hit
TAGCTTATCAGACTGATGTTGAC944201.3932375301773448No Hit
TGAGGTAGTAGGTTGTATAGTT765401.1294047930499256No Hit
TAGCTTATCAGACTGATGTTGACT751391.1087319930099082No Hit
AACCGGGCGGAAACACCA731341.0791467224315816No Hit
TATTGCACTTGTCCCGGCCTGTAA651510.9613516027174772No Hit
TCGTACGACTCTTAGCGG607620.8965886338554949No Hit
TATTGCACTTGTCCCGGCCTGTAT569700.8406348453103509No Hit
TAGCTTATCAGACTGATGTTGA517960.7642886158977521No Hit
TGTAAACATCCTTGACTGGAAGCT468590.6914394982692248No Hit
TATTGCACTTGTCCCGGCCTGT464600.6855519556454083No Hit
CAACGGAATCCCAAAAGCAGCTGT447080.659699888785943No Hit
AGCAGCATTGTACAGGGCTATGA404710.5971797933044622No Hit
TGAGGTAGTAGATTGTATAGTT375520.5541077709512778No Hit
TTCAAGTAATTCAGGATAGGTT321050.47373322290133085No Hit
ACTGGACTTGGAGTCAGAAGGC320830.47340859649099515No Hit
CGCCTGTCTGAGCGTCGCT306210.45183569594959205No Hit
TGTAAACATCCCCGACTGGAAGCT303460.44777786582039514No Hit
CATAAAGTAGAAAGCACTACT302250.4459924205635485No Hit
CAACGGAATCCCAAAAGCAGCT285010.42055351458996515No Hit
TATTGCACTTGTCCCGGCCTGTA284870.4203469341470241No Hit
CACCCGGCTGTGTGCACATGTGC284310.41952061237526045No Hit
CCCATAAAGTAGAAAGCACTAC275320.40625519678926775No Hit
TTAATGCTAATCGTGATAGGGGTT214580.3166287960447518No Hit
CATTGCACTTGTCTCGGTCTGA191920.2831922757801695No Hit
CCCCCACTGCTAAATTTGACTGGCTT165310.24392723587546802No Hit
CCCTGTCTGAGCGTCGCT164030.2420385003971509No Hit
TGTAAACATCCTACACTCTCAGCT152390.22486281214120482No Hit
CCCCCCACTGCTAAATTTGACTGGCT150650.22229531235036754No Hit
CCCATAAAGTAGAAAGCACT149400.22045084410982346No Hit
AACGGAATCCCAAAAGCAGCTG148040.21844406266411154No Hit
AACATTCAACGCTGTCGGTGAGTT146710.2164815484561727No Hit
TAGCTTATCAGACTGATGTTGACA141210.20836588819777893No Hit
TAGCAGCACGTAAATATTGGC140740.20767236813933437No Hit
TGAGGTAGTAGTTTGTACAGTT132150.19499718239031574No Hit
TTCTCACTACTGCACTTGACTAG126650.18688152213192197No Hit
AGCTCGGTCTGAGGCCCCTCAGT120890.17838221247949504No Hit
CTACGCCTGTCTGAGCGTCGCT117670.1736308622918536No Hit
TGTCAGTTTGTCAAATACCCCAA112820.16647432551854272No Hit
CTTCTCACTACTGCACTTGACTAG112580.16612018761635827No Hit
CTCCCACTGCTTCACTTGACTAG112340.16576604971417383No Hit
CAAAGAATTCTCCTTTTGGGCT110710.1633608631285044No Hit
TCGTTTCCCGGCCAATGCACCA108610.1602621564843904No Hit
TACGCCTGTCTGAGCGTCGCT107610.1587865818919552No Hit
CCCCCCACTGCTAAATTTGACTGG105120.15511240115679148No Hit
ACCCCCCACTGCTAAATTTGACTG103510.15273672606297076No Hit
TTCAAGTAATCCAGGATAGGCTT102150.1507299446172588No Hit
GCCTGTCTGAGCGTCGCA101470.14972655389440287No Hit
TGAGGTAGTAGGTTGTGTGGTT96300.14209783325151273No Hit
CAACACCAGTCGATGGGCTGT92460.13643162681656146No Hit
TGAGGTAGTAGTTTGTGCTGTT91860.1355462820611003No Hit
CTCCCACTGCTTCACTTGACTAGCCT90450.13346572188576664No Hit
CACTAGATTGTGAGCTCCTGGA89810.13252135414660807No Hit
TATTGCACTTGTCCCGGCCTGTT85130.1256156650540112No Hit
TGTAAACATCCCCGACTGGAAGC83820.12368266233792104No Hit
TGATGTAGTAGGTTGTATAGTT83740.12356461637052622No Hit
CACTAGATTGTGAGCTCCTGGAA83360.12300389802540083No Hit
AATGCACCCGGGCAAGGATTCT81980.12096760508784023No Hit
TTCACAGTGGCTAAGTTCTGC81680.12052493271010964No Hit
TTCAAGTAATCCAGGATAGGC81320.11999372585683296No Hit
AGCAGCATTGTACAGGGCTATGT81080.11963958795464852No Hit
TGAGAACTGAATTCCATGGGTT80600.11893131215027959No Hit
GCCTGTCTGAGCGTCGCTT79780.1177213409844827No Hit
TGAGAACTGAATTCCATAGGCTGT79310.11702782092603814No Hit
TGTCAGTTTGTCAAATACCCCA78090.11522761992326716No Hit
GCCTGTCTGAGCGTCGCG77780.11477019179961226No Hit
ATCACATTGCCAGGGATTTCC76910.11348644190419359No Hit
CAACGGAATCCCAAAAGCAGCTGA76350.11266012013242986No Hit
CAAAGAATTCTCCTTTTGGGCTT71240.10511993396508584No Hit
TATTGCACTTGTCCCGGCCTGTAG70350.10380667257781848No Hit
CAAAGTGCTGTTCGTGCAGGTAG69700.1028475490927356No Hit
ACGCCTGTCTGAGCGTCGCT69310.10227207500168585No Hit
TAGCAGCACGTAAATATTGGCGT68580.10119490554920813No Hit
TTCACAGTGGCTAAGTTCCGC68460.1010178365981159No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)