FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8785095
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG146566716.68356460573278No Hit
CAACGGAATCCCAAAAGCAGCTG8195659.329039697351025No Hit
CCCCCACTGCTAAATTTGACTG2902413.303788974393561No Hit
TTCAAGTAATCCAGGATAGGCT2574492.9305203870874474No Hit
AACATTCAACGCTGTCGGTGAGT2465012.8059002207716595No Hit
TAGCAGCACGTAAATATTGGCG1977082.2504935917027646No Hit
AACATTCAACGCTGTCGGTGAG1709971.9464445176745386No Hit
AAGCTGCCAGTTGAAGAACTGT1549941.7642837100794018No Hit
TATTGCACTTGTCCCGGCCTGTAA1473071.6767832334197867No Hit
TATTGCACTTGTCCCGGCCTGTAT1332371.5166256027965548No Hit
TGAGGTAGTAGGTTGTATAGTT805230.9165865593940645No Hit
TTAATGCTAATCGTGATAGGGGTT698120.7946641442124416No Hit
TAGCTTATCAGACTGATGTTGACT634880.7226785823033217No Hit
TATTGCACTTGTCCCGGCCTGT621290.7072091992175383No Hit
TAGCTTATCAGACTGATGTTGAC586540.6676535654992917No Hit
CAACGGAATCCCAAAAGCAGCTGT572300.6514442928619439No Hit
TATTGCACTTGTCCCGGCCTGTA534580.6085079330388573No Hit
TGTAAACATCCTTGACTGGAAGCT472460.5377972577416636No Hit
AACCGGGCGGAAACACCA444600.5060844532699987No Hit
TGAGGTAGTAGATTGTATAGTT420680.4788565177724316No Hit
TTCAAGTAATTCAGGATAGGTT385930.439300884054185No Hit
TAGCTTATCAGACTGATGTTGA383850.4369332374891791No Hit
CACCCGGCTGTGTGCACATGTGC381590.43436069843297087No Hit
TGTAAACATCCCCGACTGGAAGCT379810.4323345393533024No Hit
ACTGGACTTGGAGTCAGAAGGC375720.42767892663653606No Hit
CCCCCACTGCTAAATTTGACTGGCTT369670.4207922623488989No Hit
CCCCCCACTGCTAAATTTGACTGGCT367030.41778717247792996No Hit
CTCCCACTGCTTCACTTGACTAG355220.40434394847181504No Hit
GCCTGTCTGAGCGTCGCT348550.39675154338114726No Hit
AGCAGCATTGTACAGGGCTATGA342640.39002423992000085No Hit
CCCATAAAGTAGAAAGCACTAC339680.3866548967313387No Hit
GTCAGGATGGCCGAGCGGTCTAAGG331190.37699080089629083No Hit
AGCTCGGTCTGAGGCCCCTCAGT303700.3456991643232088No Hit
ACCCCCCACTGCTAAATTTGACTG286220.3258018268442174No Hit
CCCCCCACTGCTAAATTTGACTGG278550.31707113013575833No Hit
CATAAAGTAGAAAGCACTACT262260.2985283596819386No Hit
TTCTCACTACTGCACTTGACTAG261630.29781123596273No Hit
GGAATACCGGGTGCTGTAGGCTTT252030.2868836364319339No Hit
TCGTTTCCCGGGCGGCGCACCA249150.283605356572695No Hit
CTTCTCACTACTGCACTTGACTAG227600.2590751722092931No Hit
CTCCCACTGCTTCACTTGACTAGCCT216420.24634907192238673No Hit
TGTAAACATCCTACACTCTCAGCT202370.23035607469241937No Hit
GACCCGCGGGCGCTCTCCAGTCCT194500.22139771966040206No Hit
TATTGCACTTGTCCCGGCCTGTAG187440.21336138083879572No Hit
CAAAGAATTCTCCTTTTGGGCT184950.21052703471049544No Hit
AACATTCAACGCTGTCGGTGAGTT184670.21020831305751392No Hit
CTACGCCTGTCTGAGCGTCGCT184120.2095822526677287No Hit
TGAGAACTGAATTCCATGGGTT180410.20535919076572307No Hit
TGAGGGGCAGAGAGCGAGACTTT168420.19171107426840575No Hit
CATTGCACTTGTCTCGGTCTGA163280.1858602553529586No Hit
GGAATACCGGGTGCTGTAGGCTT161300.18360643794973192No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC161250.18354952336884234No Hit
TGAGGTAGTAGTTTGTACAGTT160720.18294622881141295No Hit
CACTAGATTGTGAGCTCCTGGA159520.18158027887006345No Hit
AACGGAATCCCAAAAGCAGCTG158680.18062411391111877No Hit
CACTAGATTGTGAGCTCCTGGAA155580.17709540989596584No Hit
GAATACCGGGTGCTGTAGGCTTT152440.17352117421610125No Hit
TGAGGTAGTAGGTTGTGTGGTT145490.16561004747245192No Hit
TATTGCACTTGTCCCGGCCTGTT140850.16032837436590042No Hit
TAGCAGCACGTAAATATTGGC138080.15717530658461862No Hit
CAACGGAATCCCAAAAGCAGCT129340.1472266378451229No Hit
TTCAAGTAATCCAGGATAGGC126900.1444492062977122No Hit
GGGAATACCGGGTGCTGTAGGCTTT124110.14127337268407456No Hit
CCCATAAAGTAGAAAGCACT122320.1392358306882282No Hit
TAGCAGCACATCATGGTTTACA117800.13409075257581166No Hit
TATTGCACTTGTCCCGGCCTGTAAT117050.13323703386246818No Hit
CCCGCCACTGCTAAATTTGACTG115480.1314499160225359No Hit
TATTGCACTTGTCCCGGCCTGTTT115310.13125640644751138No Hit
CGCCTGTCTGAGCGTCGCT114310.1301181148297201No Hit
TTAATGCTAATCGTGATAGGGGTTT109130.12422176424956133No Hit
CCCCCCACTGCTAAATTTGACTGA108970.12403963759071474No Hit
GGGAATACCGGGTGCTGTAGGCTT107540.12241188057727322No Hit
TCGTTTCCCGGCCAATGCACCA107240.12207039309193583No Hit
TATTGCACTTGTCCCGGCCTGTAAA107220.12204762725957999No Hit
CCCCCACTGCTAAATTTGACTGGCT106510.12123944021094821No Hit
TTCACCACCTTCTCCACCCAGC104600.11906530322096688No Hit
TTCAAGTAATCCAGGATAGGCTT104280.11870104990327367No Hit
CCCCCACTGCTAAATTTGACTGGCTA100980.11494468756456247No Hit
TACGCCTGTCTGAGCGTCGCT99770.11356735470703504No Hit
CCCCCACAACCGCGCTTGACTAG98970.11265672141280202No Hit
CCCCCCACTGCTAAATTTGACTGT97530.11101758148318258No Hit
CCCCCCACTGCTAAATTTGACTGGC96950.11035737234486366No Hit
TGTAAACATCCCCGACTGGAAGC96300.10961748279329933No Hit
ACTGGACTTGGAGTCAGAAGGCT95990.10926461239178405No Hit
TAGCTTATCAGACTGATGTTGACA94430.10748887746802967No Hit
TTAATGCTAATCGTGATAGGGGT94280.10731813372536096No Hit
ATCACATTGCCAGGGATTTCC92060.10479112633386435No Hit
GTCCGATGGTAGTGGGTTATCAGAAC90880.10344794222487065No Hit
CAAAGAATTCTCCTTTTGGGCTT90540.10306092307482162No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT88750.10102338107897524No Hit
TGAGGTAGTAGTTTGTGCTGTT88610.10086402025248446No Hit
TGATGTAGTAGGTTGTATAGTT88150.10034040610830049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)