FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6134878
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG65338110.650268839901951No Hit
CCCCCCACTGCTAAATTTGACTG5603489.133808365871335No Hit
TTCAAGTAATCCAGGATAGGCT2027803.305363203636649No Hit
AACATTCAACGCTGTCGGTGAGT1703312.7764366300356746No Hit
TAGCAGCACGTAAATATTGGCG1605342.6167431528385734No Hit
AAGCTGCCAGTTGAAGAACTGT1461472.3822315618990304No Hit
CCCCCACTGCTAAATTTGACTG1434822.3387914152490077No Hit
AACATTCAACGCTGTCGGTGAG1193241.945010153421144No Hit
TATTGCACTTGTCCCGGCCTGTAA926491.5102011808547782No Hit
TAGCTTATCAGACTGATGTTGAC833841.359179432745036No Hit
GCCTGTCTGAGCGTCGCT828091.349806793223924No Hit
TAGCTTATCAGACTGATGTTGACT802761.3085182786030953No Hit
GGAATACCGGGTGCTGTAGGCTTT802461.308029271323733No Hit
TATTGCACTTGTCCCGGCCTGTAT750281.222974605199973No Hit
TGAGGTAGTAGGTTGTATAGTT729731.1894776065636512No Hit
AACCGGGCGGAAACACCA568260.9262775885681834No Hit
TGTAAACATCCTTGACTGGAAGCT542130.8836850545357218No Hit
TAGCTTATCAGACTGATGTTGA459490.7489798493140368No Hit
GGAATACCGGGTGCTGTAGGCTT436250.7110980854060993No Hit
TATTGCACTTGTCCCGGCCTGT409930.668195846763375No Hit
CAACGGAATCCCAAAAGCAGCTGT401540.6545199431838743No Hit
AGCAGCATTGTACAGGGCTATGA395720.6450332019642444No Hit
TGAGGTAGTAGATTGTATAGTT388080.6325798165831497No Hit
ACTGGACTTGGAGTCAGAAGGC386590.6301510804289833No Hit
TGTAAACATCCCCGACTGGAAGCT369510.6023102659906195No Hit
TATTGCACTTGTCCCGGCCTGTA331310.5400433390851456No Hit
TTCAAGTAATTCAGGATAGGTT324000.5281278617113494No Hit
CCCCCACTGCTAAATTTGACTGGCTT303100.4940603545824383No Hit
CACCCGGCTGTGTGCACATGTGC269850.43986204778644333No Hit
TGAATACCGGGTGCTGTAGGCTTT258850.42193178087649014No Hit
CAACGGAATCCCAAAAGCAGCT250100.4076690685617546No Hit
CATAAAGTAGAAAGCACTACT242750.3956883902173768No Hit
TCCTGTCTGAGCGTCGCT236310.38519103395373144No Hit
CCCATAAAGTAGAAAGCACTAC221770.36149048114730237No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC208420.3397296572156773No Hit
TCGTACGACTCTTAGCGG183730.299484358124155No Hit
CGCCTGTCTGAGCGTCGCT180060.29350216907328885No Hit
CCCCCCACTGCTAAATTTGACTGGCT179370.2923774523307554No Hit
AACGGAATCCCAAAAGCAGCTG160330.26134179033389093No Hit
TTCTCACTACTGCACTTGACTAG160120.26099948523833727No Hit
GTCAGGATGGCCGAGCGGTCTAAGG156360.2548705940036623No Hit
TGTAAACATCCTACACTCTCAGCT153670.2504858287320465No Hit
CATTGCACTTGTCTCGGTCTGA150400.24515564938699677No Hit
TAGCTTATCAGACTGATGTTGACA148400.24189560085791437No Hit
AACATTCAACGCTGTCGGTGAGTT147300.24010257416691907No Hit
AGCTCGGTCTGAGGCCCCTCAGT145720.23752713582894394No Hit
CAAAGAATTCTCCTTTTGGGCT145100.2365165207849284No Hit
TGAATACCGGGTGCTGTAGGCTT137230.22368822982298914No Hit
TGAGGTAGTAGTTTGTACAGTT131810.21485349830917583No Hit
GGGAATACCGGGTGCTGTAGGCTTT131320.21405478641955064No Hit
TAGCAGCACGTAAATATTGGC131110.213712481323997No Hit
CTTCTCACTACTGCACTTGACTAG128710.2098004230890981No Hit
TCGTTTCCCGGCCAATGCACCA126210.2057253624277451No Hit
GGAATACCGGGTGCTGTAGGCTTA125870.20517115417780107No Hit
ACCCCCCACTGCTAAATTTGACTG123720.2016666020090375No Hit
CTCCCACTGCTTCACTTGACTAG116990.19069653870867523No Hit
CCCCCCACTGCTAAATTTGACTGG116500.18989782681905004No Hit
CCCATAAAGTAGAAAGCACT114480.18660517780467678No Hit
CAACGGAATCCCAAAAGCAGCTGA113550.18508925523865347No Hit
CTACGCCTGTCTGAGCGTCGCT113230.1845676474740003No Hit
TTCAAGTAATCCAGGATAGGCTT111120.18112829627581836No Hit
CACTAGATTGTGAGCTCCTGGAA104580.1704679375857189No Hit
TATTGCACTTGTCCCGGCCTGTAG97880.15954677501329287No Hit
TGTAAACATCCCCGACTGGAAGC97390.15874806312366765No Hit
TATTGCACTTGTCCCGGCCTGTAAA92730.15115215005090565No Hit
CACTAGATTGTGAGCTCCTGGA92380.15058164155831624No Hit
ACCAGGATGGCCGAGTGGTTAAGGC90670.1477943000659508No Hit
TTCAAGTAATCCAGGATAGGC90500.14751719594097876No Hit
AATGCACCCGGGCAAGGATTCT88390.14407784474279683No Hit
TACGCCTGTCTGAGCGTCGCT88330.14398004328692438No Hit
TGAGGTAGTAGGTTGTGTGGTT88010.14345843552227117No Hit
GGGAATACCGGGTGCTGTAGGCTT87310.14231741853709234No Hit
TATTGCACTTGTCCCGGCCTGTT87090.14195881319889328No Hit
TGAGGTAGTAGTTTGTGCTGTT86960.14174691004450293No Hit
CAAAGAATTCTCCTTTTGGGCTT86370.14078519572842363No Hit
TATTGCACTTGTCCCGGCCTGTAAT82580.13460740376581246No Hit
ACTGGACTTGGAGTCAGAAGGCT79250.12917942296489024No Hit
CAACACCAGTCGATGGGCTGT79220.12913052223695404No Hit
GAATACCGGGTGCTGTAGGCTTT77440.12622907904607067No Hit
AGCAGCATTGTACAGGGCTATGT77200.12583787322258078No Hit
TTCACAGTGGCTAAGTTCTGC76780.1251532630314735No Hit
CTCCCACTGCTTCACTTGACTAGCCT75520.12309943245815158No Hit
TCGTTTCCCGGGCGGCGCACCA73540.11987198441435999No Hit
ATGCACCTGGGCAAGGATTCTGA73470.11975788271584212No Hit
TTCACAGTGGCTAAGTTCCG68710.11199896721662597No Hit
ATCACATTGCCAGGGATTTCC67140.1094398291212963No Hit
CCCCCACTGCTAAATTTGACTGGCTA67090.10935832790806924No Hit
GGAATACCGGGTGCTGTAGGCT66890.109032323055161No Hit
TGAGGGGCAGAGAGCGAGACTTT66790.10886932062870687No Hit
TTAATGCTAATCGTGATAGGGGTT65890.1074022987906198No Hit
TATTGCACTTGTCCCGGCCTGTTT63150.10293603230577691No Hit
TAGCAGCACGTAAATATTGGCGT62570.10199061823234301No Hit
GTCAGGATGGCCGAGTGGTCTAAGG61490.10023019202663852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)