FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7964096
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG130103416.336242054339877No Hit
CAACGGAATCCCAAAAGCAGCTG92351811.596017928462942No Hit
CCCCCACTGCTAAATTTGACTG3941654.9492748455066335No Hit
AACATTCAACGCTGTCGGTGAGT2732653.431211778461736No Hit
AACATTCAACGCTGTCGGTGAG2148572.697820317585323No Hit
TAGCAGCACGTAAATATTGGCG1838242.3081590176713087No Hit
TTCAAGTAATCCAGGATAGGCT1756522.2055485016755196No Hit
AAGCTGCCAGTTGAAGAACTGT1558931.9574475244899108No Hit
TATTGCACTTGTCCCGGCCTGTAA1353031.698912218034539No Hit
TAGCTTATCAGACTGATGTTGACT1317461.6542492707270227No Hit
TAGCTTATCAGACTGATGTTGAC1197861.504075289901076No Hit
TATTGCACTTGTCCCGGCCTGTAT1159641.4560849090719148No Hit
TAGCTTATCAGACTGATGTTGA972851.2215447930311236No Hit
TGAGGTAGTAGGTTGTATAGTT589050.739631968273612No Hit
TATTGCACTTGTCCCGGCCTGT551070.6919429399143355No Hit
TATTGCACTTGTCCCGGCCTGTA486790.611230703396845No Hit
CAACGGAATCCCAAAAGCAGCTGT426960.536106043925136No Hit
AGCAGCATTGTACAGGGCTATGA404820.5083062785782592No Hit
CACCCGGCTGTGTGCACATGTGC399320.5014002844767316No Hit
CAACGGAATCCCAAAAGCAGCT397950.4996800641278056No Hit
ACTGGACTTGGAGTCAGAAGGC345680.4340480074574691No Hit
GCCTGTCTGAGCGTCGCT338570.42512044053713066No Hit
CGCCCCACTGCTAAATTTGACTG329940.4142843079741882No Hit
TGTAAACATCCTTGACTGGAAGCT328160.4120492771558756No Hit
TGTAAACATCCCCGACTGGAAGCT316230.3970695481320165No Hit
TGAGGTAGTAGATTGTATAGTT310110.389385060149953No Hit
TTCAAGTAATTCAGGATAGGTT297360.3733757101873207No Hit
CATTGCACTTGTCTCGGTCTGA263150.33042042687581863No Hit
CATAAAGTAGAAAGCACTACT258830.32499608241789146No Hit
ACCCCCCACTGCTAAATTTGACTG251910.3163070862028785No Hit
CCCGCCACTGCTAAATTTGACTG221710.27838690040903574No Hit
CCCCCACTGCTAAATTTGACTGGCTT209020.26245288856387466No Hit
CCCATAAAGTAGAAAGCACT200690.25199344658828826No Hit
CTTCTCACTACTGCACTTGACTAG195230.24513767789840807No Hit
CTCCCACTGCTTCACTTGACTAG194800.2445977547231977No Hit
AACGGAATCCCAAAAGCAGCTG192520.2417349062592917No Hit
TGATGTAGTAGGTTGTATAGTT189850.2383823600318228No Hit
CAACACCAGTCGATGGGCTGT186130.23371139674860775No Hit
CCCTCCACTGCTAAATTTGACTG185260.2326189940452752No Hit
TAGCTTATCAGACTGATGTTGACA180050.22607713417819175No Hit
TCCTGTCTGAGCGTCGCT179760.22571299994374755No Hit
CACTAGATTGTGAGCTCCTGGA179300.22513540770980162No Hit
TCGTACGACTCTTAGCGG168240.2112480813892751No Hit
CACTAGATTGTGAGCTCCTGGAA167360.21014312233303065No Hit
AACATTCAACGCTGTCGGTGAGTT162560.2041160729353338No Hit
TTCTCACTACTGCACTTGACTAG159020.19967112400453235No Hit
TATTGCACTTGTCCCGGCCTGTAG153970.19333016578403878No Hit
ACTTGACTTGGAGTCAGAAGGC153850.19317948954909633No Hit
CCCATAAAGTAGAAAGCACTAC149960.1882950682663795No Hit
CCCCCCACTGCTAAATTTGACTGG149950.18828251191346765No Hit
CCCCCCACTGCTAAATTTGACTGGCT147090.18469139498067325No Hit
TAGCAGCACGTAAATATTGGC145600.18282049839680486No Hit
CTCCCACTGCTTCACTTGACTAGCCT136040.1708166250130586No Hit
GCCCCCACTGCTAAATTTGACTG132230.16603265455363672No Hit
CAAAGAATTCTCCTTTTGGGCT129980.1632074751484663No Hit
TATTGCACTTGTCCCGGCCTGTAAT128580.16144958574080473No Hit
CAACGGAATCCCAAAAGCAGCTGA126990.15945312562781763No Hit
CAAGGGAATCCCAAAAGCAGCTG125910.15809703951333584No Hit
CGACGGAATCCCAAAAGCAGCTG125720.15785846880801035No Hit
TTCAAGTAATCCAGGATAGGCTT124060.1557741142246402No Hit
CAATGGAATCCCAAAAGCAGCTG118630.1489560145934956No Hit
CTCCCCACTGCTAAATTTGACTG118160.1483658660066378No Hit
TATTGCACTTGTCCCGGCCTGTT116150.14584203907135224No Hit
TGTAAACATCCTACACTCTCAGCT109910.1380068748543463No Hit
TGAGGTAGTAGTTTGTACAGTT109520.13751717709078345No Hit
ATCAGCATTGTACAGGGCTATGA107090.1344659833331994No Hit
TTATGTAGTAGGTTGTATAGTT105920.13299689004251078No Hit
TTAGGTAGTAGGTTGTATAGTT103850.13039772498975402No Hit
TGATGTAGTAGATTGTATAGTT99550.12499849323765058No Hit
TTTAAACATCCTTGACTGGAAGCT99510.12494826782600309No Hit
TATTGCACTTGTCCCGGCCTGTAAA98810.12406932312217231No Hit
CCCCCCACTGCTAAATTTGACTGT95620.12006384654328628No Hit
CAAAGAATTCTCCTTTTGGGCTT95210.11954903607389966No Hit
TTTAAACATCCCCGACTGGAAGCT92670.11635972243428507No Hit
ACCACAGGGTAGAACCACGGAC90290.11337131044126038No Hit
CGCCCACTGCTAAATTTGACTG89680.1126053729136364No Hit
ATGCACCTGGGCAAGGATTCTGA86190.1082232057473943No Hit
CCTTGGCTTGGCTCTTGACGTGGACA85640.10753260633724153No Hit
TGCAAGTAATCCAGGATAGGCT85560.1074321555139466No Hit
ACTGGACTTGGAGTCAGAAGGCT85520.10738193010229911No Hit
TATTGCACTTGTCCCGGCCTGTTT81790.10269841046617219No Hit
CCCCCCACTGCTAAATTTGACTGTA81140.10188224752690073No Hit
AGCAGCATTGTACAGGGCTATGT79880.1003001470600053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)