FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003947779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23482720
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG18304057.794689030912943No Hit
CAACGGAATCCCAAAAGCAGCTG13249965.642429837770071No Hit
AACATTCAACGCTGTCGGTGAGT11486784.891588367957374No Hit
AAGCTGCCAGTTGAAGAACTGT6883472.9312916050610833No Hit
TAGCAGCACGTAAATATTGGCG6703802.8547800254825675No Hit
TTCAAGTAATCCAGGATAGGCT6540812.785371541286529No Hit
CCCCCACTGCTAAATTTGACTG5785162.4635817315881634No Hit
AACATTCAACGCTGTCGGTGAG4860712.069909277971206No Hit
CGCCTGTCTGAGCGTCGCT4093861.7433500037474365No Hit
TAGCTTATCAGACTGATGTTGAC3103191.3214780911240265No Hit
TAGCTTATCAGACTGATGTTGA2767731.1786241116872322No Hit
TATTGCACTTGTCCCGGCCTGT2684401.1431384439281311No Hit
TCGTACGACTCTTAGCGG2638801.1237199097889852No Hit
TAGCTTATCAGACTGATGTTGACT2311550.984362118187331No Hit
TGAGGTAGTAGGTTGTATAGTT2190350.9327496985017067No Hit
TATTGCACTTGTCCCGGCCTGTAA2103930.8959481695476503No Hit
TATTGCACTTGTCCCGGCCTGTAT1967800.8379778833116436No Hit
CATAAAGTAGAAAGCACTACT1665640.7093045439369885No Hit
AGCAGCATTGTACAGGGCTATGA1579250.6725157903343395No Hit
ACTGGACTTGGAGTCAGAAGGC1533520.6530418963390953No Hit
TGTAAACATCCTTGACTGGAAGCT1377370.5865461922639285No Hit
TATTGCACTTGTCCCGGCCTGTA1353120.5762194498763346No Hit
TGAGGTAGTAGATTGTATAGTT1018510.43372743872941466No Hit
TACGCCTGTCTGAGCGTCGCT983100.41864826561829294No Hit
CTACGCCTGTCTGAGCGTCGCT979780.41723446006254816No Hit
AACATTCAACGCTGTCGGTGAGTT922010.39263339170249445No Hit
TGTAAACATCCCCGACTGGAAGCT916290.390197558034163No Hit
TTCAAGTAATTCAGGATAGGTT907730.38655232443260407No Hit
ACGCCTGTCTGAGCGTCGCT902250.3842186935755313No Hit
CCCATAAAGTAGAAAGCACTAC828660.35288075657334417No Hit
CAACGGAATCCCAAAAGCAGCTGT811500.34557325556834984No Hit
CATTGCACTTGTCTCGGTCTGA777710.3311839514332241No Hit
GCGTTCGCGCTTTCCCCTG725000.3087376590105405No Hit
ACCGGGTGCTGTAGGCTT689610.293667002800357No Hit
GCCTGTCTGAGCGTCGCT682010.29043058044383274No Hit
CCCATAAAGTAGAAAGCACT633960.26996872593975485No Hit
TTAATGCTAATCGTGATAGGGGTT624670.2660126254539508No Hit
TTCTCACTACTGCACTTGACTAG600940.25590732249075065No Hit
CAACACCAGTCGATGGGCTGT590630.2515168600570973No Hit
CACCCGGCTGTGTGCACATGTGC575320.24499717238888852No Hit
TGTAAACATCCTACACTCTCAGCT567220.24154782750890869No Hit
ATCCCGGACGAGCCCCCA526020.22400301157617175No Hit
CTCCCACTGCTTCACTTGACTAG511140.21766643727813476No Hit
CTTCTCACTACTGCACTTGACTAG502360.21392751776625535No Hit
TGATGTAGTAGGTTGTATAGTT490720.20897068142021027No Hit
CCCCCCACTGCTAAATTTGACTGGCT485530.20676054562674173No Hit
AGCTCGGTCTGAGGCCCCTCAGT472480.201203267764552No Hit
ACCGGGTGCTGTAGGCTTT446560.1901653641486165No Hit
TGAGGTAGTAGTTTGTACAGTT445460.1896969345970143No Hit
TTCAAGTAATCCAGGATAGGCTT436820.1860176333917025No Hit
CCCCCCACTGCTAAATTTGACTGG434260.1849274700716101No Hit
TACCTGGTTGATCCTGCC427910.1822233540237247No Hit
TATTGCACTTGTCCCGGCCTGTT408730.17405564602396997No Hit
CCTCGACACAAGGGTTTGT399570.1701549053942644No Hit
ACCACAGGGTAGAACCACGGAC397910.16944800261639198No Hit
TGAGGTAGTAGGTTGTGTGGTT392900.16731451893136742No Hit
ACTTGACTTGGAGTCAGAAGGC385460.16414623178234888No Hit
TCGTTCGCGCTTTCCCCTG383580.16334564309415603No Hit
CAACGGAATCCCAAAAGCAGCT382880.16304755156131828No Hit
AACGGAATCCCAAAAGCAGCTG380530.1620468157010772No Hit
TGAGGTAGTAGTTTGTGCTGTT371810.1583334468920125No Hit
CCCCCACTGCTAAATTTGACTGGCTT365180.155510094230992No Hit
CACTAGATTGTGAGCTCCTGGA359060.15290392254389612No Hit
ATCCCACCGCTGCCACCA341500.14542608352013736No Hit
ACCGGGTGCTGTAGGCTTA333880.14218114426267484No Hit
AGCAGCATTGTACAGGGCTATGT326100.13886806979770658No Hit
AACATTCAACGCTGTCGGTGAGA324620.13823781912827815No Hit
TTATAAAGCAATGAGACTGATT322810.13746703959336906No Hit
TCCTGTCTGAGCGTCGCT319820.13619376290310492No Hit
AACATTCAACGCTGTCGGTGA315920.13453296722015165No Hit
TAGCTTATCAGACTGATGTTGACA310000.13201196454243802No Hit
TTCAAGTAATCCAGGATAGGC309430.1317692328656987No Hit
AACCGGGCGGAAACACCA305690.1301765723902512No Hit
CCAAGTCCTTCTGATCGAGGCCC304550.12969110903677256No Hit
TTCACAGTGGCTAAGTTCCG302340.12874999148309907No Hit
CAAAGTGCTGTTCGTGCAGGT301840.1285370689596435No Hit
TAGCAGCACGTAAATATTGGC298210.12699125143935627No Hit
AGCAGCATTGTACAGGGCTATG295520.12584572826316542No Hit
TAGTGGGTTATCAGAACT289220.12316290446762554No Hit
GGGCTACGCCTGTCTGAGCGTCGCT284240.12104219613400832No Hit
CTCCCACTGCTTCACTTGACTAGCCT280900.11961987367732528No Hit
ACTGGACTTGGAGTCAGAAGG274160.11674967806114453No Hit
TAGCAGCACGTAAATATTGGCGT273480.11646010342924498No Hit
TCGTTTCCCGGCCAATGCACCA273390.116421777375023No Hit
CTCGACACAAGGGTTTGT273380.11641751892455389No Hit
TATTGCACTTGTCCCGGCCTGTAG273370.11641326047408476No Hit
CTTGACTAGCTTGCTGTTT262800.11191207832823456No Hit
ACTGGACTTGGAGTCAGAAGGCA260180.1107963643053275No Hit
TGTAAACATCCTACACTCAGCT257500.10965509957960577No Hit
ACTGGACTTGGAGTCAGAAGGCT254350.10831368768183583No Hit
CCCGCCACTGCTAAATTTGACTG253360.10789210108539385No Hit
TGTAAACATCCCCGACTGGAAGC244310.1040382034108485No Hit
CAAAGTGCTGTTCGTGCAGGTAG242380.10321632247031007No Hit
GCTTGACTAGCTTGCTGTTT242360.10320780556937185No Hit
CACTAGATTGTGAGCTCCTGGAA234990.10006932757363712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)