FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12110358
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG149726212.363482565915888No Hit
CAACGGAATCCCAAAAGCAGCTG10138698.371916007767895No Hit
AACATTCAACGCTGTCGGTGAGT4560933.766139696283132No Hit
CCCCCACTGCTAAATTTGACTG3953023.264164445014755No Hit
TTCAAGTAATCCAGGATAGGCT3105172.5640612771315263No Hit
TAGCAGCACGTAAATATTGGCG3067552.5329969601228965No Hit
AACATTCAACGCTGTCGGTGAG2919112.4104242005067067No Hit
AAGCTGCCAGTTGAAGAACTGT2441542.0160758253389375No Hit
TATTGCACTTGTCCCGGCCTGTAT1898191.567410311074206No Hit
TATTGCACTTGTCCCGGCCTGTAA1851561.5289060818846147No Hit
TCGTACGACTCTTAGCGG1533451.2662301147497044No Hit
TAGCTTATCAGACTGATGTTGAC1518241.2536706181600907No Hit
TGAGGTAGTAGGTTGTATAGTT1466351.2108229996173523No Hit
TATTGCACTTGTCCCGGCCTGT1399821.1558865559548281No Hit
TAGCTTATCAGACTGATGTTGACT1394401.151411048294361No Hit
TAGCTTATCAGACTGATGTTGA1181050.9752395428772626No Hit
TATTGCACTTGTCCCGGCCTGTA917980.7580122734604543No Hit
AGCAGCATTGTACAGGGCTATGA766580.6329953251588434No Hit
TGAGGTAGTAGATTGTATAGTT762010.6292216960060141No Hit
ACTGGACTTGGAGTCAGAAGGC738610.6098993935604545No Hit
TGTAAACATCCTTGACTGGAAGCT683350.5642690331697874No Hit
TTCAAGTAATTCAGGATAGGTT680440.5618661314554037No Hit
CGCCTGTCTGAGCGTCGCT631940.5218177695490092No Hit
CATAAAGTAGAAAGCACTACT572710.4729092236579629No Hit
CAACGGAATCCCAAAAGCAGCTGT532230.4394832919059866No Hit
CACCCGGCTGTGTGCACATGTGC500780.41351378712338643No Hit
GCCTGTCTGAGCGTCGCT480950.39713937440990593No Hit
TGTAAACATCCCCGACTGGAAGCT479860.39623931844128807No Hit
CCCATAAAGTAGAAAGCACTAC420580.34728948557920414No Hit
TTAATGCTAATCGTGATAGGGGTT419690.34655457749473634No Hit
ACCGGGTGCTGTAGGCTT410650.3390898931311527No Hit
CATTGCACTTGTCTCGGTCTGA402900.33269041262033705No Hit
CTCCCACTGCTTCACTTGACTAG378280.31236070808146216No Hit
ACCGGGTGCTGTAGGCTTA378210.31230290632200963No Hit
ATCCCGGACGAGCCCCCA347370.2868371025860672No Hit
CCCCCCACTGCTAAATTTGACTGGCT327030.27004156276800406No Hit
TGAGGTAGTAGTTTGTACAGTT304030.2510495560907448No Hit
ACCGGGTGCTGTAGGCTTT301340.2488283170489262No Hit
AACGGAATCCCAAAAGCAGCTG296200.24458401642626915No Hit
CAACACCAGTCGATGGGCTGT295640.24412160235064892No Hit
CCCCCACTGCTAAATTTGACTGGCTT290570.23993510348744437No Hit
CACTAGATTGTGAGCTCCTGGA288540.23825885246332107No Hit
TCGTTTCCCGGGCGGCGCACCA287340.23726796515842058No Hit
AACATTCAACGCTGTCGGTGAGTT287190.23714410424530802No Hit
AGCTCGGTCTGAGGCCCCTCAGT281980.23284200186319842No Hit
TATTGCACTTGTCCCGGCCTGTAG268900.22204133023978317No Hit
CCCATAAAGTAGAAAGCACT267720.22106695772329768No Hit
TATTGCACTTGTCCCGGCCTGTT264520.21842459157689642No Hit
ACCCCCCACTGCTAAATTTGACTG255330.21083604630020022No Hit
CACTAGATTGTGAGCTCCTGGAA250580.2069137840516358No Hit
TTCTCACTACTGCACTTGACTAG240400.19850775674839669No Hit
TGAGGTAGTAGGTTGTGTGGTT237460.1960800828513905No Hit
TGTAAACATCCTACACTCTCAGCT234270.19344597409919673No Hit
CCCCCCACTGCTAAATTTGACTGG232870.19228993891014617No Hit
CTCCCACTGCTTCACTTGACTAGCCT232810.19224039454490113No Hit
TGAGGTAGTAGTTTGTGCTGTT210720.17399981074052476No Hit
CTTCTCACTACTGCACTTGACTAG209670.17313278434873686No Hit
TAGCTTATCAGACTGATGTTGACA201240.16617180103181095No Hit
GACCCGCGGGCGCTCTCCAGTCCT196020.16186144125549384No Hit
ATCCCACCGCTGCCACCA192630.15906218461914998No Hit
TTCAAGTAATCCAGGATAGGCTT186160.15371965056689488No Hit
TAGCAGCACGTAAATATTGGC185220.1529434555113895No Hit
CTACGCCTGTCTGAGCGTCGCT182610.15078827562323094No Hit
GGAATACCGGGTGCTGTAGGCTTT177640.14668435070210145No Hit
GAATACCGGGTGCTGTAGGCTTT176740.14594118522342608No Hit
TTCACCACCTTCTCCACCCAGC174630.14419887504564274No Hit
TACGCCTGTCTGAGCGTCGCT173750.14347222435538237No Hit
TTCAAGTAATCCAGGATAGGC169420.13989677266353315No Hit
ACCACAGGGTAGAACCACGGAC166840.1377663649579971No Hit
CAACGGAATCCCAAAAGCAGCT166290.13731220827658439No Hit
TATTGCACTTGTCCCGGCCTGTTT163340.13487627698537072No Hit
AGCAGCATTGTACAGGGCTATGT155830.1286749739355352No Hit
GGAATACCGGGTGCTGTAGGCTT154730.12776666057270974No Hit
CAAAGTGCTGTTCGTGCAGGTAG151160.1248187708406308No Hit
TGAGAACTGAATTCCATGGGTT150510.12428204021714305No Hit
CCCCCCACTGCTAAATTTGACTGA145960.12052492585272871No Hit
ACTGGACTTGGAGTCAGAAGGCT144790.11955881073045073No Hit
CCCCCCACTGCTAAATTTGACTGT140950.11638797135476919No Hit
TATTGCACTTGTCCCGGCCTGTAAT140690.11617327910537409No Hit
TGAGGTAGTAGGTTGTATAGT138020.11396855485197052No Hit
AACCGGGCGGAAACACCA134680.11121058518666418No Hit
TCGTTTCCCGGCCAATGCACCA133650.11036007358329125No Hit
CAAAGTGCTGTTCGTGCAGGT132950.10978205598876599No Hit
GAATACCGGGTGCTGTAGGCTT129320.10678462189144201No Hit
TGAGGGGCAGAGAGCGAGACTTT127680.10543040924141135No Hit
CAAAGAATTCTCCTTTTGGGCT127530.1053065483282988No Hit
ACGCCTGTCTGAGCGTCGCT125520.1036468120925905No Hit
AGCAGCATTGTACAGGGCTATG123070.101623750511752No Hit
TATTGCACTTGTCCCGGCCTGTAAA122940.10151640438705446No Hit
ACTGGACTTGGAGTCAGAAGGCA121820.100591576235814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)