FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10203747
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG9541329.350800250143402No Hit
CAACGGAATCCCAAAAGCAGCTG9033948.853551543369313No Hit
TTCAAGTAATCCAGGATAGGCT3473863.4044944469908947No Hit
AAGCTGCCAGTTGAAGAACTGT3000822.940900043876039No Hit
TAGCAGCACGTAAATATTGGCG2954122.895132542976614No Hit
AACATTCAACGCTGTCGGTGAGT2595842.5440066281533635No Hit
CCCCCACTGCTAAATTTGACTG2519872.469553586540317No Hit
GCCTGTCTGAGCGTCGCT2235482.190842246480631No Hit
TCCACCACGTTCCCGTGG1644271.6114374454795872No Hit
AACATTCAACGCTGTCGGTGAG1518261.4879435956222749No Hit
TGAGGTAGTAGGTTGTATAGTT1306691.2805981959372377No Hit
TAGCTTATCAGACTGATGTTGAC1266701.2414067106916704No Hit
TAGCTTATCAGACTGATGTTGA1002540.9825214208074741No Hit
TATTGCACTTGTCCCGGCCTGTAA992030.9722212830247555No Hit
TAGCTTATCAGACTGATGTTGACT862450.8452287184305922No Hit
TATTGCACTTGTCCCGGCCTGTAT858560.8414163934092054No Hit
TATTGCACTTGTCCCGGCCTGT802200.7861817820453605No Hit
CGCCTGTCTGAGCGTCGCT720470.7060837553106716No Hit
AGCAGCATTGTACAGGGCTATGA685460.6717728301181909No Hit
TGAGGTAGTAGATTGTATAGTT642570.6297392516690192No Hit
ACTGGACTTGGAGTCAGAAGGC613540.6012889186687989No Hit
GTCAGGATGGCCGAGCGGTCTAAGG604120.59205701591778No Hit
CCCCCACTGCTAAATTTGACTGGCTT537180.5264536645214744No Hit
CATAAAGTAGAAAGCACTACT522060.5116355785771638No Hit
TGTAAACATCCTTGACTGGAAGCT504820.4947398244978046No Hit
CAACGGAATCCCAAAAGCAGCTGT493480.48362626003957176No Hit
TATTGCACTTGTCCCGGCCTGTA485470.4757762026047882No Hit
CTACGCCTGTCTGAGCGTCGCT451060.44205329669581184No Hit
CCCATAAAGTAGAAAGCACTAC438720.429959700098405No Hit
TTCAAGTAATTCAGGATAGGTT393310.385456440658515No Hit
CCCCCCACTGCTAAATTTGACTGGCT377310.3697759264317314No Hit
TCCTGTCTGAGCGTCGCT372710.3652677785915311No Hit
TAGCAGCACATCATGGTTTACA353040.3459905464139791No Hit
TACGCCTGTCTGAGCGTCGCT339760.33297571960574873No Hit
GGGCTACGCCTGTCTGAGCGTCGCT320060.31366908646402153No Hit
TCAGGCTCAGTCCCCTCCCGAT319840.31345347939340323No Hit
CATTGCACTTGTCTCGGTCTGA317840.3114934151150553No Hit
TCGTACGACTCTTAGCGG314100.30782809491454466No Hit
TGTAAACATCCCCGACTGGAAGCT306070.2999584368369776No Hit
TCGTTTCCCGGCCAATGCACCA299890.2939018382168825No Hit
CCCCCCACTGCTAAATTTGACTGG286930.2812006216931878No Hit
CACCCGGCTGTGTGCACATGTGC278290.2727331440107247No Hit
CTCCCACTGCTTCACTTGACTAG276410.27089068358907764No Hit
TTCACAGTGGCTAAGTTCCGC262170.25693502592724027No Hit
TGAGGTAGTAGTTTGTACAGTT255720.2506138186295681No Hit
AGCTCGGTCTGAGGCCCCTCAGT255550.25044721316590857No Hit
AACGGAATCCCAAAAGCAGCTG253120.24806573506771581No Hit
TTCTCACTACTGCACTTGACTAG250510.24550785118447174No Hit
TGTAAACATCCTACACTCTCAGCT217170.21283357966441152No Hit
ACGCCTGTCTGAGCGTCGCT215880.21156933820487708No Hit
GTCAGGATGGCCGAGTGGTCTAAGG215420.21111852342085707No Hit
TCAGGCTCAGTCCCCTCCCGATA212770.20852143825204603No Hit
CTTCTCACTACTGCACTTGACTAG211740.20751200514869686No Hit
AACATTCAACGCTGTCGGTGAGTT209230.20505212447937018No Hit
TGAGGTAGTAGTTTGTGCTGTT198980.19500679505283697No Hit
TGAGGTAGTAGGTTGTGTGGTT197850.19389935873557038No Hit
TTCACAGTGGCTAAGTTCCG190150.18635311126393078No Hit
TAGCTTATCAGACTGATGTTGACA176970.17343628766961786No Hit
ATCACATTGCCAGGGATTTCC176040.17252485778018603No Hit
ACCCCCCACTGCTAAATTTGACTG175630.17212304460312472No Hit
GCGTTCGCGCTTTCCCCTG173520.17005517678946763No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC163160.15990204382762527No Hit
TTCAAGTAATCCAGGATAGGC161320.15809878469154517No Hit
CCCATAAAGTAGAAAGCACT160780.15756956733639124No Hit
TCAGGCTCAGTCCCCTCCCGA160300.15709915190958773No Hit
ACCGGGTGCTGTAGGCTT157410.15426685902737494No Hit
TATTGCACTTGTCCCGGCCTGTT145280.14237906917919468No Hit
AACCGGGCGGAAACACCA141930.13909596151296186No Hit
TAGCAGCACGTAAATATTGGC141750.13891955572791054No Hit
TTCAAGTAATCCAGGATAGGCTT139940.13714569755600564No Hit
CTCCCACTGCTTCACTTGACTAGCCT139370.13658707923667648No Hit
CAACGGAATCCCAAAAGCAGCT138360.1355972467761108No Hit
ATCACATTGCCAGGGATTTCCA138080.13532283777714207No Hit
GACCCGCGGGCGCTCTCCAGTCCT137470.13472501817224594No Hit
CAACACCAGTCGATGGGCTGT132710.13006006518977783No Hit
CAAAGTGCTGTTCGTGCAGGTAG128360.12579692538437107No Hit
GCCCCCACTGCTAAATTTGACTG126580.1240524681766414No Hit
TTCACAGTGGCTAAGTTCTGC125210.12270982414597305No Hit
AGCAGCATTGTACAGGGCTATGT125060.12256281932509695No Hit
CAAAGAATTCTCCTTTTGGGCT125050.12255301900370522No Hit
TAGCAGCACGTAAATATTGGCGT123540.12107317047355252No Hit
TGAGGTAGTAGGTTGTATAGT123220.12075956018901685No Hit
TCAGGCTCAGTCCCCTCCCGAAA121640.11921110940912197No Hit
TCGTTTCCCGGGCGGCGCACCA119640.11725104513077401No Hit
TATTGCACTTGTCCCGGCCTGTAG117220.114879367353973No Hit
ACCACAGGGTAGAACCACGGAC115190.11288990211144985No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT114050.11177266547279152No Hit
AGCAGCATTGTACAGGGCTATG113800.11152765743799803No Hit
AGAGGTAGTAGGTTGCATAGTT111970.10973419862330965No Hit
CCCCCACTGCTAAATTTGACTGGCT107140.10500064339109938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)