FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7944488
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG87055010.957911951028185No Hit
CAACGGAATCCCAAAAGCAGCTG4611055.804087060110104No Hit
AAGCTGCCAGTTGAAGAACTGT2610803.2863036611044034No Hit
CCCCCACTGCTAAATTTGACTG2318282.9180986867876193No Hit
TTCAAGTAATCCAGGATAGGCT2282652.8732499816224784No Hit
TAGCAGCACGTAAATATTGGCG2176592.7397486156439532No Hit
AACATTCAACGCTGTCGGTGAGT2173402.735733253042864No Hit
TAGCTTATCAGACTGATGTTGAC1312111.6515979380924233No Hit
TATTGCACTTGTCCCGGCCTGTAA1275701.60576742012827No Hit
GCCTGTCTGAGCGTCGCT1161281.461743034919305No Hit
TATTGCACTTGTCCCGGCCTGTAT1095071.3784022330954493No Hit
AACATTCAACGCTGTCGGTGAG1089551.3714540194409004No Hit
TAGCTTATCAGACTGATGTTGACT985691.2407218690493333No Hit
TGTAAACATCCTTGACTGGAAGCT852351.0728822297925302No Hit
TATTGCACTTGTCCCGGCCTGT823281.0362908220139548No Hit
AGCAGCATTGTACAGGGCTATGA695780.8758021914061673No Hit
TAGCTTATCAGACTGATGTTGA694800.8745686317356134No Hit
TGAGGTAGTAGGTTGTATAGTT661080.8321241091936951No Hit
TATTGCACTTGTCCCGGCCTGTA579070.7288953045180507No Hit
AACCGGGCGGAAACACCA547900.6896605545882881No Hit
TGTAAACATCCCCGACTGGAAGCT481840.6065085629180886No Hit
TGAGGTAGTAGATTGTATAGTT464890.5851730155549356No Hit
CGCCTGTCTGAGCGTCGCT454630.5722584010448503No Hit
ACTGGACTTGGAGTCAGAAGGC435660.5483802102791269No Hit
CTCCCACTGCTTCACTTGACTAG431660.5433452728482944No Hit
CTTCTCACTACTGCACTTGACTAG427820.5385117329146951No Hit
CATAAAGTAGAAAGCACTACT417290.5252572601280284No Hit
TTCAAGTAATTCAGGATAGGTT394320.4963441319314725No Hit
TTCTCACTACTGCACTTGACTAG384390.4838448997594307No Hit
TCCTGTCTGAGCGTCGCT322990.4065586101961511No Hit
CCCATAAAGTAGAAAGCACTAC308620.3884705974758852No Hit
CAACGGAATCCCAAAAGCAGCTGT307870.38752654670760406No Hit
TGTAAACATCCTACACTCTCAGCT274810.34591278884177307No Hit
CACCCGGCTGTGTGCACATGTGC273050.34369741637220674No Hit
CCCCCCACTGCTAAATTTGACTGG242420.3051423829956065No Hit
CCCCCCACTGCTAAATTTGACTGGCT231640.2915732266195128No Hit
ACCCCCCACTGCTAAATTTGACTG230380.2899872213288005No Hit
CTACGCCTGTCTGAGCGTCGCT220510.27756351321822126No Hit
TCGTACGACTCTTAGCGG220500.27755092587464414No Hit
AGCTCGGTCTGAGGCCCCTCAGT210440.2648880582361003No Hit
GGAATACCGGGTGCTGTAGGCTTT206460.2598782954924219No Hit
CATTGCACTTGTCTCGGTCTGA203120.25567412273767676No Hit
AACATTCAACGCTGTCGGTGAGTT195780.246435012552099No Hit
CTCCCACTGCTTCACTTGACTAGCCT194880.2453021516301617No Hit
CCCCCACTGCTAAATTTGACTGGCTT182890.23020992668124113No Hit
TACGCCTGTCTGAGCGTCGCT176690.22240577366345066No Hit
TAGCTTATCAGACTGATGTTGACA176580.22226731288410279No Hit
TGAGGTAGTAGTTTGTACAGTT169450.2132925369136438No Hit
TGATGTAGTAGGTTGTATAGTT164800.20743942215030092No Hit
TTCAAGTAATCCAGGATAGGCTT157490.19823807399545446No Hit
CCCATAAAGTAGAAAGCACT153560.1932912479696615No Hit
CAAAGAATTCTCCTTTTGGGCT151600.19082412862855355No Hit
TATTGCACTTGTCCCGGCCTGTAG151030.1901066500446599No Hit
TGTAAACATCCCCGACTGGAAGC143570.1807164917361572No Hit
TATTGCACTTGTCCCGGCCTGTT141390.17797245083635346No Hit
CAAAGAATTCTCCTTTTGGGCTT140560.1769277013194557No Hit
ACGCCTGTCTGAGCGTCGCT138540.17438505791688527No Hit
CAACGGAATCCCAAAAGCAGCT135640.17073472827953168No Hit
GGAATACCGGGTGCTGTAGGCTT130220.16391238806075356No Hit
AGCAGCATTGTACAGGGCTATGT127450.16042569388990202No Hit
CCCCCCACTGCTAAATTTGACTGTA121710.15320055867665733No Hit
TGAGGTAGTAGTTTGTGCTGTT117690.14814044655867062No Hit
CCCCCCACTGCTAAATTTGACTGT116600.14676842610876875No Hit
TGATGTAGTAGATTGTATAGTT115980.1459880108069897No Hit
GCGTTCGCGCTTTCCCCTG114680.14435165614196913No Hit
CACTAGATTGTGAGCTCCTGGA113690.14310550912783807No Hit
TGTAAACATCCTACACTCAGCT112950.14217404570313405No Hit
TAGCAGCACGTAAATATTGGC111850.14078943790965512No Hit
ACCACAGGGTAGAACCACGGAC108740.1368747740571828No Hit
TTATAAAGCAATGAGACTGATT107720.13559086501232048No Hit
ACTGGACTTGGAGTCAGAAGGCA104730.13182724928277317No Hit
ACTTGACTTGGAGTCAGAAGGC104340.13133634288326698No Hit
CACTAGATTGTGAGCTCCTGGAA103770.13061886429937336No Hit
TAGCAGCACGTAAATATTGGCGT101970.1283531424554987No Hit
GTGCGGTAACGCGACCGATCCCGGA101550.1278244740252613No Hit
AACGGAATCCCAAAAGCAGCTG98520.12401050892140564No Hit
TTCAAGTAATCCAGGATAGGC98490.12397274689067439No Hit
CCAAGTCCTTCTGATCGAGGCCC97820.12312939487100995No Hit
TGTCAGTTTGTCAAATACCCCA96230.121128007242254No Hit
TATTGCACTTGTCCCGGCCTGTTT96190.12107765786794568No Hit
CAACACCAGTCGATGGGCTGT94660.11915179430065223No Hit
TCCCACTGCTTCACTTGACTAG94170.11853501446537525No Hit
AGCAGCATTGTACAGGGCTATG93350.11750285229205457No Hit
ATCACATTGCCAGGGATTTCCA93060.1171378193283192No Hit
TATTGCACTTGTCCCGGCCTGTAAT92120.11595460903207355No Hit
CAAAGTGCTGTTCGTGCAGGTAG91230.11483433545371331No Hit
TGTCAGTTTGTCAAATACCCCAA90590.1140287454647801No Hit
CGCGACCTCAGATCAGACGTGGCG87120.10966093724353287No Hit
CCCCCCACTGCTAAATTTGACTGA86240.10855325100874971No Hit
ACCGGGTGCTGTAGGCTT85680.10784835976843316No Hit
GGGCTACGCCTGTCTGAGCGTCGCT82660.10404698200815458No Hit
TCGTTTCCCGGGCGGCGCACCA82030.10325397936279845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)