FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6004479
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5331778.879654671121342No Hit
CAACGGAATCCCAAAAGCAGCTG4786587.971682472367711No Hit
CCCCCACTGCTAAATTTGACTG1902153.1678851737178193No Hit
TTCAAGTAATCCAGGATAGGCT1888063.1444193576162065No Hit
GCCTGTCTGAGCGTCGCT1692842.819295395986896No Hit
AAGCTGCCAGTTGAAGAACTGT1536672.559206219224016No Hit
TAGCAGCACGTAAATATTGGCG1530062.548197770364423No Hit
AACATTCAACGCTGTCGGTGAGT1492622.485844317217197No Hit
AACATTCAACGCTGTCGGTGAG1102881.836762190358231No Hit
TATTGCACTTGTCCCGGCCTGTAA790631.3167337249409983No Hit
TAGCTTATCAGACTGATGTTGAC746201.2427389620315101No Hit
TATTGCACTTGTCCCGGCCTGT719671.1985552784846112No Hit
TGAGGTAGTAGGTTGTATAGTT688651.146893843745644No Hit
TATTGCACTTGTCCCGGCCTGTAT647281.0779952765260732No Hit
TAGCTTATCAGACTGATGTTGACT580470.9667283372962083No Hit
TAGCTTATCAGACTGATGTTGA527630.8787273633565876No Hit
TGAGGTAGTAGATTGTATAGTT434610.7238096760768087No Hit
TATTGCACTTGTCCCGGCCTGTA425090.7079548450415099No Hit
TTCAAGTAATTCAGGATAGGTT411890.6859712557908854No Hit
TGTAAACATCCTTGACTGGAAGCT380270.6333105669950715No Hit
GGAATACCGGGTGCTGTAGGCTTT376080.6263324428314263No Hit
TCCTGTCTGAGCGTCGCT371520.6187381119993924No Hit
AACCGGGCGGAAACACCA357260.5949891739150057No Hit
CATAAAGTAGAAAGCACTACT351750.5858126908262982No Hit
CACCCGGCTGTGTGCACATGTGC320450.533684937527469No Hit
TCGTACGACTCTTAGCGG305100.5081206879064778No Hit
AGCAGCATTGTACAGGGCTATGA301330.5018420415826252No Hit
ACTGGACTTGGAGTCAGAAGGC285560.47557831412184143No Hit
CAACGGAATCCCAAAAGCAGCT279870.4661020548160798No Hit
CTCCCACTGCTTCACTTGACTAG270110.44984752215804236No Hit
CAACGGAATCCCAAAAGCAGCTGT263290.43848933437855303No Hit
TTCTCACTACTGCACTTGACTAG243850.40611350293672444No Hit
GGAATACCGGGTGCTGTAGGCTT236400.39370609839754617No Hit
CGCCTGTCTGAGCGTCGCT232130.38659474035965485No Hit
TGTAAACATCCCCGACTGGAAGCT231390.3853623270228774No Hit
CATTGCACTTGTCTCGGTCTGA210690.350888062061671No Hit
CCCATAAAGTAGAAAGCACTAC195790.32607325298331463No Hit
ACCGGGTGCTGTAGGCTT166930.27800913284899487No Hit
CCCCCACTGCTAAATTTGACTGGCTT160840.2678667041720023No Hit
TTAATGCTAATCGTGATAGGGGTT159870.266251243446767No Hit
TGAGGTAGTAGTTTGTACAGTT156150.2600558682943183No Hit
CTTCTCACTACTGCACTTGACTAG153050.2548930556672777No Hit
ACCCCCCACTGCTAAATTTGACTG147000.24481724392740817No Hit
CCCATAAAGTAGAAAGCACT135570.22578145414448117No Hit
ACCGGGTGCTGTAGGCTTT134820.22453238657342295No Hit
CTCCCACTGCTTCACTTGACTAGCCT134470.2239494883735958No Hit
CCCCCCACTGCTAAATTTGACTGGCT133120.22170116674569101No Hit
ACCGGGTGCTGTAGGCTTA131150.21842028259237814No Hit
TGAGAACTGAATTCCATGGGTT130360.21710459808419683No Hit
AACGGAATCCCAAAAGCAGCTG130130.21672155069573898No Hit
AGCTCGGTCTGAGGCCCCTCAGT127830.21289107681116046No Hit
CCCCCCACTGCTAAATTTGACTGG127170.21179189734862924No Hit
TCGTTTCCCGGCCAATGCACCA124750.20776157265268144No Hit
TGAGGTAGTAGTTTGTGCTGTT116450.1939385581996373No Hit
CAACACCAGTCGATGGGCTGT112440.1872602102530461No Hit
TATTGCACTTGTCCCGGCCTGTT111760.18612772232195332No Hit
TAGCAGCACGTAAATATTGGC104570.17415332787407534No Hit
CTACGCCTGTCTGAGCGTCGCT103460.17230470786890917No Hit
TGATGTAGTAGGTTGTATAGTT100210.16689208172765696No Hit
TAGCTTATCAGACTGATGTTGACA99910.1663924546992337No Hit
CACTAGATTGTGAGCTCCTGGA98370.1638277026199942No Hit
AACATTCAACGCTGTCGGTGAGTT97730.1627618316260245No Hit
TATTGCACTTGTCCCGGCCTGTAG93230.1552674261996753No Hit
TGAGGTAGTAGGTTGTGTGGTT92430.15393508745721318No Hit
TGAATACCGGGTGCTGTAGGCTTT90870.15133702690941214No Hit
TGTAAACATCCTACACTCTCAGCT90600.15088736258383117No Hit
TTCAAGTAATCCAGGATAGGCTT89770.14950506113852677No Hit
TTCAAGTAATCCAGGATAGGC86420.14392589265446676No Hit
TTCACAGTGGCTAAGTTCTGC77060.12833752936766035No Hit
CAAAGAATTCTCCTTTTGGGCT74850.12465694359160887No Hit
AATGCACCCGGGCAAGGATTCT74690.12439047584311645No Hit
CACTAGATTGTGAGCTCCTGGAA74600.12424058773458946No Hit
TACGCCTGTCTGAGCGTCGCT74500.12407404539178171No Hit
TTATAAAGCAATGAGACTGATT72520.12077650700418804No Hit
TCCCACTGCTTCACTTGACTAG71780.11954409366741062No Hit
CAACGGAATCCCAAAAGCAGCTGA71120.11844491420487939No Hit
GTGCGGTAACGCGACCGATCCCGGA71070.11836164303347552No Hit
GGGAATACCGGGTGCTGTAGGCTTT70100.11674618230824024No Hit
CCCCCCACTGCTAAATTTGACTGT69260.11534722662865503No Hit
CCCCCCACTGCTAAATTTGACTGTA68790.11456447761745857No Hit
TGTAAACATCCCCGACTGGAAGC67700.11274916608085399No Hit
TAGTGGGTTATCAGAACT66850.11133355616698801No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC65360.10885207525915237No Hit
GAATACCGGGTGCTGTAGGCTTT65280.10871884138490616No Hit
CGCGACCTCAGATCAGACGTGG63680.106054163899982No Hit
ACCACAGGGTAGAACCACGGAC63230.10530472335734707No Hit
ACGCCTGTCTGAGCGTCGCT63210.10527141488878551No Hit
TGATGTAGTAGATTGTATAGTT62150.10350606605502326No Hit
GGGAATACCGGGTGCTGTAGGCTT62040.10332286947793473No Hit
TTCACAGTGGCTAAGTTCCGC61990.10323959830653084No Hit
CGCGACCTCAGATCAGACGTGGCG61910.10310636443228464No Hit
GGAATACCGGGTGCTGTAGGCTTA60270.10037507001023735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)