FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7058543
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG93511513.247988997162729No Hit
CAACGGAATCCCAAAAGCAGCTG6505019.215797084469132No Hit
CCCCCACTGCTAAATTTGACTG2965344.20106529066976No Hit
TTCAAGTAATCCAGGATAGGCT2026622.871159104648084No Hit
AAGCTGCCAGTTGAAGAACTGT1971212.7926584849026206No Hit
TAGCAGCACGTAAATATTGGCG1750662.480200233957631No Hit
AACATTCAACGCTGTCGGTGAGT1616542.290189349275056No Hit
GCCTGTCTGAGCGTCGCT1562282.2133179609446314No Hit
AACATTCAACGCTGTCGGTGAG1088161.5416212665985034No Hit
TATTGCACTTGTCCCGGCCTGTAA959401.3592040170329769No Hit
TAGCTTATCAGACTGATGTTGAC804111.1392011070839974No Hit
TATTGCACTTGTCCCGGCCTGTAT743731.0536593741796287No Hit
TAGCTTATCAGACTGATGTTGACT741161.0500183961477603No Hit
TGAGGTAGTAGGTTGTATAGTT738571.0463490836564997No Hit
TATTGCACTTGTCCCGGCCTGT606950.859880006397921No Hit
TCCTGTCTGAGCGTCGCT500220.7086731638526534No Hit
TGTAAACATCCTTGACTGGAAGCT491940.6969426976643763No Hit
TAGCTTATCAGACTGATGTTGA487360.6904541064636144No Hit
AGCAGCATTGTACAGGGCTATGA408090.5781504766635267No Hit
TATTGCACTTGTCCCGGCCTGTA402850.5707268483028297No Hit
TGAGGTAGTAGATTGTATAGTT397420.5630340425779088No Hit
CAACGGAATCCCAAAAGCAGCTGT379650.5378588754081401No Hit
CACCCGGCTGTGTGCACATGTGC355120.5031066609638845No Hit
CGCCTGTCTGAGCGTCGCT350030.4958955410486272No Hit
TTAATGCTAATCGTGATAGGGGTT349950.49578220321105926No Hit
ACTGGACTTGGAGTCAGAAGGC342230.48484510188575747No Hit
CATAAAGTAGAAAGCACTACT319580.4527563266243473No Hit
TTCAAGTAATTCAGGATAGGTT318230.450843750615389No Hit
TGTAAACATCCCCGACTGGAAGCT282800.4006492558025077No Hit
TCGTACGACTCTTAGCGG282020.39954421188622075No Hit
CAACGGAATCCCAAAAGCAGCT258310.36595371027703594No Hit
CCCATAAAGTAGAAAGCACTAC253120.35860091806481875No Hit
AACCGGGCGGAAACACCA244980.3470687930922855No Hit
CCCCCACTGCTAAATTTGACTGGCTT229090.3245570651053624No Hit
TTCTCACTACTGCACTTGACTAG221940.3144274958727318No Hit
CTCCCACTGCTTCACTTGACTAG214650.30409958542435744No Hit
ACCCCCCACTGCTAAATTTGACTG204120.2891814925544833No Hit
CTTCTCACTACTGCACTTGACTAG182230.2581694267499681No Hit
CATTGCACTTGTCTCGGTCTGA178740.25322506358606867No Hit
CCCCCCACTGCTAAATTTGACTGGCT176230.249669088932376No Hit
CCCCCCACTGCTAAATTTGACTGG168440.23863281699920222No Hit
AGCTCGGTCTGAGGCCCCTCAGT160340.2271573609454529No Hit
AACGGAATCCCAAAAGCAGCTG153650.2176794842788377No Hit
GGAATACCGGGTGCTGTAGGCTTT152670.216291095768631No Hit
CCCATAAAGTAGAAAGCACT150300.2129334623306821No Hit
CTCCCACTGCTTCACTTGACTAGCCT138370.19603195730336984No Hit
TGAGGTAGTAGTTTGTACAGTT138360.19601779007367381No Hit
CTACGCCTGTCTGAGCGTCGCT134560.19063424278919885No Hit
TGTAAACATCCTACACTCTCAGCT128790.18245975125461444No Hit
TACGCCTGTCTGAGCGTCGCT120930.1713243087135688No Hit
ACCGGGTGCTGTAGGCTT120160.17023343202697783No Hit
TAGCAGCACGTAAATATTGGC118210.1674708222362604No Hit
AACATTCAACGCTGTCGGTGAGTT117090.16588409251030986No Hit
CAAAGAATTCTCCTTTTGGGCT113490.16078388981975458No Hit
TCGTTTCCCGGCCAATGCACCA111790.15837546077143683No Hit
CAACACCAGTCGATGGGCTGT110520.1565762226000465No Hit
TTCAAGTAATCCAGGATAGGCTT106060.15025763815563636No Hit
TAGCTTATCAGACTGATGTTGACA105980.15014430031806847No Hit
TATTGCACTTGTCCCGGCCTGTAG104770.14843006552485405No Hit
TGAGGTAGTAGTTTGTGCTGTT104770.14843006552485405No Hit
CACTAGATTGTGAGCTCCTGGA101770.144179896616058No Hit
ACCGGGTGCTGTAGGCTTT101550.14386821756274631No Hit
GGAATACCGGGTGCTGTAGGCTT98660.1397738881806061No Hit
TATTGCACTTGTCCCGGCCTGTT98220.13915053007398268No Hit
ACCGGGTGCTGTAGGCTTA97710.13842800135948735No Hit
CACTAGATTGTGAGCTCCTGGAA96250.13635958582387328No Hit
CAACGGAATCCCAAAAGCAGCTGA93140.1319535773884214No Hit
ACGCCTGTCTGAGCGTCGCT90260.12787341523597717No Hit
AATGCACCCGGGCAAGGATTCT87170.12349574125991723No Hit
TGAGAACTGAATTCCATGGGTT85620.12129982065703929No Hit
TGAGGTAGTAGGTTGTGTGGTT84220.11931640849960111No Hit
TTCAAGTAATCCAGGATAGGC84110.11916056897294527No Hit
CAAAGAATTCTCCTTTTGGGCTT82180.11642629364161981No Hit
TTCACAGTGGCTAAGTTCTGC77550.1098668662923779No Hit
CCCCCCACTGCTAAATTTGACTGT77190.10935684602332238No Hit
TGATGTAGTAGGTTGTATAGTT76970.10904516697001067No Hit
TGTAAACATCCCCGACTGGAAGC76690.10864848453852304No Hit
GTGCGGTAACGCGACCGATCCCGGA76230.10799679197250762No Hit
TATTGCACTTGTCCCGGCCTGTAAT75510.1069767514343966No Hit
ACCACAGGGTAGAACCACGGAC74720.10585754028841364No Hit
CACCCGTAGAACCGACCTTGCG74320.10529085110057416No Hit
GGGAATACCGGGTGCTGTAGGCTTT71800.10172070921718548No Hit
TATTGCACTTGTCCCGGCCTGTAAA71430.10119652171843396No Hit
CCCCCCACTGCTAAATTTGACTGTA70920.10047399300393864No Hit
GCCCCCACTGCTAAATTTGACTG70750.10023315009910685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)