FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8052793
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG101200712.567155271469165No Hit
CAACGGAATCCCAAAAGCAGCTG7767179.645311881231766No Hit
CCCCCACTGCTAAATTTGACTG3380804.198294926989927No Hit
TTCAAGTAATCCAGGATAGGCT2598923.227352298761436No Hit
TAGCAGCACGTAAATATTGGCG2482613.082917939154775No Hit
AAGCTGCCAGTTGAAGAACTGT1909622.3713759933975704No Hit
GCCTGTCTGAGCGTCGCT1892762.350439158190208No Hit
AACATTCAACGCTGTCGGTGAGT1625162.018132094044886No Hit
AACATTCAACGCTGTCGGTGAG1335181.6580334301403252No Hit
TATTGCACTTGTCCCGGCCTGTAA1024551.2722914894248494No Hit
TATTGCACTTGTCCCGGCCTGT1020461.2672125062695638No Hit
TATTGCACTTGTCCCGGCCTGTAT905651.1246408544215654No Hit
TGAGGTAGTAGGTTGTATAGTT785280.9751647658147924No Hit
TCGTACGACTCTTAGCGG737950.9163901270031404No Hit
TCCTGTCTGAGCGTCGCT662280.8224227296044988No Hit
TATTGCACTTGTCCCGGCCTGTA623110.7737812209999686No Hit
TAGCTTATCAGACTGATGTTGAC506780.629322025289859No Hit
TAGCTTATCAGACTGATGTTGA460080.5713297237368451No Hit
CATAAAGTAGAAAGCACTACT456480.566859225116056No Hit
CAACGGAATCCCAAAAGCAGCTGT425050.5278292885462224No Hit
TAGCTTATCAGACTGATGTTGACT417510.5184660775460141No Hit
TTAATGCTAATCGTGATAGGGGTT408650.5074636837181832No Hit
TGAGGTAGTAGATTGTATAGTT404680.5025337171835909No Hit
CACCCGGCTGTGTGCACATGTGC398610.4949959597868715No Hit
TGTAAACATCCTTGACTGGAAGCT384320.47725056387268366No Hit
CTCCCACTGCTTCACTTGACTAG359540.4464786316995855No Hit
TTCAAGTAATTCAGGATAGGTT333360.41396817228506927No Hit
ACTGGACTTGGAGTCAGAAGGC317850.394707774060503No Hit
TTCTCACTACTGCACTTGACTAG317360.39409928952600665No Hit
GGAATACCGGGTGCTGTAGGCTTT304760.3784525443532449No Hit
AGCAGCATTGTACAGGGCTATGA303590.3769996323014884No Hit
AACCGGGCGGAAACACCA293250.36415936681844424No Hit
CATTGCACTTGTCTCGGTCTGA281710.34982893512847035No Hit
CCCATAAAGTAGAAAGCACTAC277630.3447623700249094No Hit
TGTAAACATCCCCGACTGGAAGCT268280.3331514916625822No Hit
CTTCTCACTACTGCACTTGACTAG250840.3114944094552039No Hit
GGAATACCGGGTGCTGTAGGCTT240840.29907635773078983No Hit
CCCCCACTGCTAAATTTGACTGGCTT239980.29800840528249023No Hit
TGAGAACTGAATTCCATGGGTT230380.28608707562705266No Hit
ACCGGGTGCTGTAGGCTT224690.279021204195861No Hit
ACCCCCCACTGCTAAATTTGACTG220110.27333373650607934No Hit
CAACGGAATCCCAAAAGCAGCT204630.25411059243668627No Hit
AACGGAATCCCAAAAGCAGCTG203170.2522975568849218No Hit
CCCCCCACTGCTAAATTTGACTGG200110.24849763305725106No Hit
ACCGGGTGCTGTAGGCTTT193930.24082327709156315No Hit
ACCGGGTGCTGTAGGCTTA191460.23775601831563287No Hit
CCCATAAAGTAGAAAGCACT189790.2356822036776557No Hit
CACTAGATTGTGAGCTCCTGGA186880.2320685506258512No Hit
AGCTCGGTCTGAGGCCCCTCAGT184340.22891436548785No Hit
CCCCCCACTGCTAAATTTGACTGGCT165080.20499719786662837No Hit
TATTGCACTTGTCCCGGCCTGTT160790.19966985367685475No Hit
TAGCAGCACGTAAATATTGGC150610.18702827702140115No Hit
TATTGCACTTGTCCCGGCCTGTAG137700.1709965722451825No Hit
TGAGGTAGTAGTTTGTACAGTT135880.16873648683133916No Hit
CTCCCACTGCTTCACTTGACTAGCCT135450.16820251060718933No Hit
TTCAAGTAATCCAGGATAGGC133000.16516008793470788No Hit
CAACACCAGTCGATGGGCTGT132160.1641169715898571No Hit
ATCCCGGACGAGCCCCCA131690.1635333231588096No Hit
CACTAGATTGTGAGCTCCTGGAA130220.16170786955532074No Hit
TGAGGTAGTAGTTTGTGCTGTT127970.15891380791732757No Hit
TGTAAACATCCTACACTCTCAGCT123850.15379757060686894No Hit
TCGTTTCCCGGCCAATGCACCA123070.15282896257236464No Hit
TCGTTTCCCGGGCGGCGCACCA121690.1511152714343955No Hit
TGAATACCGGGTGCTGTAGGCTTT121060.1503329341757574No Hit
CAAAGAATTCTCCTTTTGGGCT118250.14684346164119702No Hit
CTACGCCTGTCTGAGCGTCGCT113470.1409076329169271No Hit
TGATGTAGTAGGTTGTATAGTT111430.13837435036514661No Hit
TTCACAGTGGCTAAGTTCCG109850.13641229819268919No Hit
CGCCTGTCTGAGCGTCGCT109190.13559270677887783No Hit
AACATTCAACGCTGTCGGTGAGTT108450.1346737709512712No Hit
TATTGCACTTGTCCCGGCCTGA103510.1285392533994106No Hit
CGCGACCTCAGATCAGACGTGG101430.1259562986407325No Hit
TGAGGTAGTAGGTTGTGTGGTT100960.12537265020968502No Hit
CCCATAAAGTAGAAAGCACTA100330.12459031295104692No Hit
TGAATACCGGGTGCTGTAGGCTT97840.1214982180716678No Hit
TGTAAACATCCCCGACTGGAAGC93610.11624538219224063No Hit
GACCCGCGGGCGCTCTCCAGTCCT93180.11571140596809083No Hit
TTAATGCTAATCGTGATAGGGGT91440.11355066496804277No Hit
TCCCACTGCTTCACTTGACTAG86040.10684491703685914No Hit
CCCCCCACTGCTAAATTTGACTGT83050.10313191957125932No Hit
CCCTGTCTGAGCGTCGCT82790.10280905022642456No Hit
GTGCGGTAACGCGACCGATCCCGGA81450.10114503129535306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)