FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7888677
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT6949868.809918317101841No Hit
CCCCCCACTGCTAAATTTGACTG5450316.909029232658403No Hit
CAACGGAATCCCAAAAGCAGCTG3258864.131060252561994No Hit
AAGCTGCCAGTTGAAGAACTGT2939653.726416989819713No Hit
TTCAAGTAATCCAGGATAGGCT2100792.6630447665685892No Hit
AACATTCAACGCTGTCGGTGAGT2058422.6093348732620187No Hit
TAGCAGCACGTAAATATTGGCG2002012.5378273188267184No Hit
CCCCCACTGCTAAATTTGACTG1959512.4839526323615484No Hit
TAGCTTATCAGACTGATGTTGAC1205931.5286847211515948No Hit
TGAGGTAGTAGGTTGTATAGTT998091.2652184897416894No Hit
TATTGCACTTGTCCCGGCCTGT923791.1710328614037562No Hit
AACATTCAACGCTGTCGGTGAG845541.0718400563237662No Hit
TAGCTTATCAGACTGATGTTGA843981.0698625384205742No Hit
TAGCTTATCAGACTGATGTTGACT807771.0239613055522492No Hit
CGCCTGTCTGAGCGTCGCT726470.9209021994435823No Hit
TATTGCACTTGTCCCGGCCTGTAT687370.8713374878956256No Hit
TGAGGTAGTAGATTGTATAGTT654730.8299617286903748No Hit
CATAAAGTAGAAAGCACTACT653960.8289856461355941No Hit
TGTAAACATCCTTGACTGGAAGCT632050.8012116607131969No Hit
TATTGCACTTGTCCCGGCCTGTAA631160.8000834613966322No Hit
TCGTACGACTCTTAGCGG588420.7459045414078939No Hit
TTCAAGTAATTCAGGATAGGTT561080.7112472725147703No Hit
AACCGGGCGGAAACACCA520930.6603515393012035No Hit
AGCAGCATTGTACAGGGCTATGA502380.6368368232087586No Hit
TTCTCACTACTGCACTTGACTAG411600.5217604929191549No Hit
TATTGCACTTGTCCCGGCCTGTA391210.4959133198126885No Hit
ACCGGGTGCTGTAGGCTT336190.4261677845347199No Hit
GGAATACCGGGTGCTGTAGGCTTT323500.4100814369760608No Hit
CATTGCACTTGTCTCGGTCTGA290810.3686422957867333No Hit
ACTGGACTTGGAGTCAGAAGGC288410.3655999605510531No Hit
ACCGGGTGCTGTAGGCTTT284540.3606941949835188No Hit
TGTAAACATCCCCGACTGGAAGCT282740.35841244355675866No Hit
CTTCTCACTACTGCACTTGACTAG268420.3402598433172001No Hit
CCCATAAAGTAGAAAGCACTAC261520.33151312951461953No Hit
ACCGGGTGCTGTAGGCTTA253330.32113116052286084No Hit
CTCCCACTGCTTCACTTGACTAG248650.31519860681328443No Hit
TGAGGTAGTAGTTTGTACAGTT233910.29651359790748183No Hit
CTACGCCTGTCTGAGCGTCGCT229420.29082189573739675No Hit
CAACGGAATCCCAAAAGCAGCTGT228680.28988384237306203No Hit
TACGCCTGTCTGAGCGTCGCT223410.28320338125138095No Hit
CACCCGGCTGTGTGCACATGTGC219080.2777145014303412No Hit
TGTAAACATCCTACACTCTCAGCT218590.2770933579863899No Hit
GGAATACCGGGTGCTGTAGGCTT214180.2715030669908275No Hit
GCGTTCGCGCTTTCCCCTG203700.25821820312835725No Hit
AACATTCAACGCTGTCGGTGAGTT199710.25316032079903894No Hit
AGCTCGGTCTGAGGCCCCTCAGT182080.23081183321360474No Hit
ACGCCTGTCTGAGCGTCGCT181910.2305963344677441No Hit
TGAGGTAGTAGTTTGTGCTGTT174370.22103833126898212No Hit
CCCATAAAGTAGAAAGCACT172310.21842699352502326No Hit
ATCCCGGACGAGCCCCCA152490.1933023750370309No Hit
CAACACCAGTCGATGGGCTGT150160.19034877457905808No Hit
TCCTGTCTGAGCGTCGCT148750.18856140262809595No Hit
TTATAAAGCAATGAGACTGATT147520.18700220581980984No Hit
TGATGTAGTAGGTTGTATAGTT146290.18544300901152375No Hit
CCCCCCACTGCTAAATTTGACTGG145260.1841373401395443No Hit
TTCAAGTAATCCAGGATAGGCTT143260.18160206077647748No Hit
CGCGACCTCAGATCAGACGTGG140410.17798928768410724No Hit
GCCTGTCTGAGCGTCGCTT131720.16697349885158183No Hit
TATTGCACTTGTCCCGGCCTGTT130250.1651100685197277No Hit
CGCGACCTCAGATCAGACGTGGCG127740.16192829291907881No Hit
ACCCCCCACTGCTAAATTTGACTG120480.15272522883114623No Hit
GGGCTACGCCTGTCTGAGCGTCGCT118200.14983501035725003No Hit
CCCCCCACTGCTAAATTTGACTGGCT116690.14792087443813454No Hit
GACCCGCGGGCGCTCTCCAGTCCT113940.14443486531391767No Hit
CAACGGAATCCCAAAAGCAGCT107290.13600506143172042No Hit
TGAGGTAGTAGGTTGTGTGGTT106440.13492756770241704No Hit
AGCAGCATTGTACAGGGCTATGT106140.13454727579795697No Hit
TAGCTTATCAGACTGATGTTGACA104150.1320246728317055No Hit
TTCAAGTAATCCAGGATAGGC103630.1313655001973081No Hit
TTCACAGTGGCTAAGTTCCG103070.1306556219756494No Hit
CAAAGTGCTGTTCGTGCAGGTAG102670.13014856610303605No Hit
CCCCCACTGCTAAATTTGACTGGCTT102590.13004715492851335No Hit
ACCACAGGGTAGAACCACGGAC101790.12903304318328662No Hit
TGAGGTAGTAGGTTGTATAGT99990.12675129175652647No Hit
CAAAGTGCTGTTCGTGCAGGT99040.12554703405906972No Hit
CTCGACACAAGGGTTTGT98170.12444418753613565No Hit
CCCCCCACTGCTAAATTTGACTGT97540.1236455745367696No Hit
TGTAAACATCCTACACTCAGCT96420.12222581809345216No Hit
AGCAGCATTGTACAGGGCTATG96000.12169340942720813No Hit
GTGCGGTAACGCGACCGATCCCGGA94040.11920883565140264No Hit
TGATGTAGTAGATTGTATAGTT93580.11862572139789726No Hit
TTCACAGTGGCTAAGTTCTGC93290.11825810589025257No Hit
CACTAGATTGTGAGCTCCTGGA93270.1182327530966219No Hit
CAAAGAATTCTCCTTTTGGGCTT91670.11620452960616842No Hit
TGTCAGTTTGTCAAATACCCCAA91090.11546929859087905No Hit
CCTCGACACAAGGGTTTGT89630.11361854465584027No Hit
GAATACCGGGTGCTGTAGGCTTT89280.11317487076730358No Hit
TATTGCACTTGTCCCGGCCTGTAC89220.11309881238641156No Hit
TGTAAACATCCCCGACTGGAAGC87620.11107058889595808No Hit
ATCCCACCGCTGCCACCA86640.10982830200805534No Hit
CAAAGAATTCTCCTTTTGGGCT84390.10697611272460517No Hit
TGTCAGTTTGTCAAATACCCCA83860.10630426369339244No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT82730.10487183085325967No Hit
TAGCAGCACGTAAATATTGGC82030.10398448307618628No Hit
AACGGAATCCCAAAAGCAGCTG81880.10379433712395626No Hit
TAGCAGCACGTAAATATTGGCGT81150.10286896015643687No Hit
AGAGGTAGTAGGTTGCATAGTT79240.10044776836470805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)