FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7026759
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG105836915.061979498656495No Hit
CAACGGAATCCCAAAAGCAGCTG6265628.916799338073215No Hit
CCCCCACTGCTAAATTTGACTG3570005.080578400369217No Hit
AAGCTGCCAGTTGAAGAACTGT2299033.271821333277546No Hit
TTCAAGTAATCCAGGATAGGCT2161903.0766673511927762No Hit
TAGCAGCACGTAAATATTGGCG2083232.9647096193280573No Hit
AACATTCAACGCTGTCGGTGAGT1567402.230615850066866No Hit
TAGCTTATCAGACTGATGTTGAC1043421.484923561488305No Hit
AACATTCAACGCTGTCGGTGAG987221.4049435877906158No Hit
TAGCTTATCAGACTGATGTTGACT956221.3608265204484742No Hit
GCCTGTCTGAGCGTCGCT804271.1445817339117508No Hit
TGAGGTAGTAGGTTGTATAGTT781201.1117500970219698No Hit
TATTGCACTTGTCCCGGCCTGTAA726941.034530997861176No Hit
TGTAAACATCCTTGACTGGAAGCT670920.9548071877803124No Hit
TATTGCACTTGTCCCGGCCTGTAT607450.8644810502252888No Hit
TAGCTTATCAGACTGATGTTGA552000.7855684249310386No Hit
TATTGCACTTGTCCCGGCCTGT432530.615546939919243No Hit
TGAGGTAGTAGATTGTATAGTT419530.5970462342596352No Hit
CAACGGAATCCCAAAAGCAGCTGT415300.5910263892642398No Hit
AGCAGCATTGTACAGGGCTATGA369900.5264162325760711No Hit
AACCGGGCGGAAACACCA365280.5198413664108873No Hit
TTCAAGTAATTCAGGATAGGTT343820.48930097076048856No Hit
TCCTGTCTGAGCGTCGCT343500.48884556877502133No Hit
TTCTCACTACTGCACTTGACTAG341710.4862981639188138No Hit
TGTAAACATCCCCGACTGGAAGCT305040.43411194264667397No Hit
TATTGCACTTGTCCCGGCCTGTA302650.4307106590677153No Hit
CATAAAGTAGAAAGCACTACT302010.42979985509678076No Hit
CCCCCACTGCTAAATTTGACTGGCTT287470.4091075273821117No Hit
TTAATGCTAATCGTGATAGGGGTT276400.3933534649473534No Hit
ACTGGACTTGGAGTCAGAAGGC273230.38884213902881826No Hit
CCCATAAAGTAGAAAGCACTAC264480.37638974098869765No Hit
CACCCGGCTGTGTGCACATGTGC253820.36121916234781926No Hit
CAACGGAATCCCAAAAGCAGCT253200.3603368210009764No Hit
CTTCTCACTACTGCACTTGACTAG239940.34146610122817644No Hit
ACCCCCCACTGCTAAATTTGACTG238610.33957333672607815No Hit
CCCCCCACTGCTAAATTTGACTGG202950.28882447797056937No Hit
CCCCCCACTGCTAAATTTGACTGGCT181590.2584263954406292No Hit
CATTGCACTTGTCTCGGTCTGA164920.23470279826019363No Hit
CAAAGAATTCTCCTTTTGGGCT164250.23374930035312155No Hit
TGTAAACATCCTACACTCTCAGCT156860.2232323607512368No Hit
TAGCTTATCAGACTGATGTTGACA148010.21063764959065767No Hit
TGAGGTAGTAGTTTGTACAGTT147080.20931413757039338No Hit
TAGCAGCACGTAAATATTGGC143640.20441856622662027No Hit
AACATTCAACGCTGTCGGTGAGTT138730.19743099201210687No Hit
GGAATACCGGGTGCTGTAGGCTTT135180.19237887623582936No Hit
AGCTCGGTCTGAGGCCCCTCAGT133760.19035802992531833No Hit
TGAGAACTGAATTCCATGGGTT132890.1891199057773292No Hit
CTCCCACTGCTTCACTTGACTAG131120.18660096354521338No Hit
CAAAGAATTCTCCTTTTGGGCTT130610.18587516663087492No Hit
AACGGAATCCCAAAAGCAGCTG127850.18194732450621973No Hit
TTCAAGTAATCCAGGATAGGCTT117510.16723214785080862No Hit
TCGTACGACTCTTAGCGG115820.1648270561150596No Hit
CCCATAAAGTAGAAAGCACT110460.15719907285848284No Hit
CTCCCACTGCTTCACTTGACTAGCCT108020.15372663271929493No Hit
CGCCTGTCTGAGCGTCGCT107530.15302929842904817No Hit
CTACGCCTGTCTGAGCGTCGCT106990.15226080757857213No Hit
TTCACAGTGGCTAAGTTCTGC103080.14669636456864396No Hit
TGAGGTAGTAGTTTGTGCTGTT101370.14426281020880324No Hit
GACCCGCGGGCGCTCTCCAGTCCT99240.14123154074303673No Hit
AATGCACCCGGGCAAGGATTCT96390.13717561680996887No Hit
CCCCCCACTGCTAAATTTGACTGT95430.13580941085356704No Hit
CCCCCCACTGCTAAATTTGACTGTA94190.1340447281598814No Hit
TATTGCACTTGTCCCGGCCTGTT85840.12216158260159485No Hit
CAACACCAGTCGATGGGCTGT85800.12210465735341144No Hit
TCGTTTCCCGGCCAATGCACCA85380.12150694224748566No Hit
TGTAAACATCCCCGACTGGAAGC85270.12135039781498128No Hit
CAACGGAATCCCAAAAGCAGCTGA84200.1198276474260751No Hit
CGCGACCTCAGATCAGACGTGGCG83100.11826220310103136No Hit
CACTAGATTGTGAGCTCCTGGA82650.11762179405896801No Hit
ACCGGGTGCTGTAGGCTTT82320.1171521607614549No Hit
GGAATACCGGGTGCTGTAGGCTT81790.11639790122302474No Hit
TATTGCACTTGTCCCGGCCTGTAG81520.11601365579778672No Hit
GTGCGGTAACGCGACCGATCCCGGA81290.11568633562073212No Hit
ACCGGGTGCTGTAGGCTT80960.115216702323219No Hit
CGCCCCACTGCTAAATTTGACTG79490.11312469945247874No Hit
ACCGGGTGCTGTAGGCTTA79390.11298238633202021No Hit
CACTAGATTGTGAGCTCCTGGAA78200.11128886019856381No Hit
ATGCACCTGGGCAAGGATTCTGA77390.11013612392284979No Hit
GGGAATACCGGGTGCTGTAGGCTTT76030.10820066548461388No Hit
ATCACATTGCCAGGGATTTCC74860.1065356019752492No Hit
TTCAAGTAATCCAGGATAGGC74140.10551094750794784No Hit
CACCCGTAGAACCGACCTTGCG72060.10255083460241059No Hit
TGAGAACTGAATTCCATAGGCTGT71620.10192465687239309No Hit
GCCCCCACTGCTAAATTTGACTG70560.10041613779553275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)