FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8695945
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8228499.462444852169604No Hit
CAACGGAATCCCAAAAGCAGCTG6582737.56988458413663No Hit
GCCTGTCTGAGCGTCGCT5956606.849859330987029No Hit
CCCCCACTGCTAAATTTGACTG3597144.136571700947971No Hit
TTCAAGTAATCCAGGATAGGCT2719743.127595678215536No Hit
AAGCTGCCAGTTGAAGAACTGT2661123.0601849482718673No Hit
TAGCAGCACGTAAATATTGGCG2195602.5248549755087No Hit
AACATTCAACGCTGTCGGTGAGT1701511.956670609117238No Hit
AACATTCAACGCTGTCGGTGAG1374651.5807942667530672No Hit
TGAGGTAGTAGGTTGTATAGTT1231721.4164303017095898No Hit
TATTGCACTTGTCCCGGCCTGT879031.0108504596107726No Hit
CATAAAGTAGAAAGCACTACT779960.8969237960911666No Hit
ACCGGGTGCTGTAGGCTT683760.7862975214309658No Hit
TATTGCACTTGTCCCGGCCTGTAA668420.7686571154716365No Hit
TGAGGTAGTAGATTGTATAGTT655370.753650120832181No Hit
TCGTACGACTCTTAGCGG638390.734123778381763No Hit
TAGCTTATCAGACTGATGTTGAC634830.7300299162425705No Hit
TATTGCACTTGTCCCGGCCTGTAT628920.723233645107001No Hit
ACCGGGTGCTGTAGGCTTA626800.7207957272038864No Hit
ACCGGGTGCTGTAGGCTTT611620.7033393150485657No Hit
TAGCTTATCAGACTGATGTTGA599750.689689274713674No Hit
TAGCTTATCAGACTGATGTTGACT550180.6326856943092442No Hit
TTCAAGTAATTCAGGATAGGTT471680.5424137342175003No Hit
CGCCTGTCTGAGCGTCGCT470480.5410337806874354No Hit
TGTAAACATCCTTGACTGGAAGCT455210.5234738720173598No Hit
TATTGCACTTGTCCCGGCCTGTA445910.5127792321593571No Hit
TTCTCACTACTGCACTTGACTAG392490.451348300845969No Hit
TGAGAACTGAATTCCATGGGTT368850.42416321630369097No Hit
CAACGGAATCCCAAAAGCAGCTGT366450.4214033092435612No Hit
CACCCGGCTGTGTGCACATGTGC347970.4001520248805622No Hit
AGCAGCATTGTACAGGGCTATGA341640.3928727700094699No Hit
TTAATGCTAATCGTGATAGGGGTT332430.382281626666222No Hit
CCCATAAAGTAGAAAGCACTAC330900.38052218591538933No Hit
ATCCCGGACGAGCCCCCA313130.3600873740576786No Hit
CATTGCACTTGTCTCGGTCTGA298580.343355437505642No Hit
CAACACCAGTCGATGGGCTGT267460.30756864262595957No Hit
TGAGGTAGTAGTTTGTACAGTT261020.3001628920146114No Hit
AACCGGGCGGAAACACCA251910.2896867447988689No Hit
CCCATAAAGTAGAAAGCACT245280.28206250154526047No Hit
ACTGGACTTGGAGTCAGAAGGC231150.2658135487287465No Hit
CTCCCACTGCTTCACTTGACTAG224170.2577868190288692No Hit
GGAATACCGGGTGCTGTAGGCTTT223940.25752232793560675No Hit
TGTAAACATCCCCGACTGGAAGCT210940.24257283135990393No Hit
CTTCTCACTACTGCACTTGACTAG180820.2079359977552756No Hit
ACCCCCCACTGCTAAATTTGACTG177130.20369264065032608No Hit
TGATGTAGTAGGTTGTATAGTT174930.20116272584520717No Hit
AACGGAATCCCAAAAGCAGCTG171470.1971838598335201No Hit
GGAATACCGGGTGCTGTAGGCTT171320.19701136564226202No Hit
CTACGCCTGTCTGAGCGTCGCT167900.19307849808157712No Hit
AGCTCGGTCTGAGGCCCCTCAGT159500.18341882337112297No Hit
GACCCGCGGGCGCTCTCCAGTCCT156310.1797504469037005No Hit
TGAGGTAGTAGTTTGTGCTGTT155390.1786924825306508No Hit
CCCCCACTGCTAAATTTGACTGGCTT149170.17153972339981452No Hit
TTCACAGTGGCTAAGTTCCG146650.16864182098667826No Hit
TAGCAGCACGTAAATATTGGC145690.16753785816262637No Hit
TGAGGTAGTAGGTTGTGTGGTT143040.1644904607837331No Hit
CCCCCCACTGCTAAATTTGACTGG142560.16393847937170714No Hit
TTCAAGTAATCCAGGATAGGC142520.163892480920705No Hit
CAACGGAATCCCAAAAGCAGCT141840.1631105072536682No Hit
TGTAAACATCCTACACTCTCAGCT130870.15049543206632515No Hit
CGCGACCTCAGATCAGACGTGG127960.14714904475591786No Hit
TATTGCACTTGTCCCGGCCTGTT127290.14637857070163163No Hit
AACATTCAACGCTGTCGGTGAGTT126580.14556209819634325No Hit
ACGCCTGTCTGAGCGTCGCT126170.14509061407357107No Hit
TACGCCTGTCTGAGCGTCGCT125960.14484912220580973No Hit
TTCACAGTGGCTAAGTTCTGC124270.14290568765096837No Hit
TGAGGTAGTAGGTTGTATAGT119830.13779985958972832No Hit
TTCACAGTGGCTAAGTTCTG107130.12319535139654171No Hit
TTCACAGTGGCTAAGTTCCGC104250.11988346292438601No Hit
AGAGGTAGTAGGTTGCATAGTT100230.11526061859866868No Hit
CAAAGTGCTGTTCGTGCAGGT98380.11313319023981867No Hit
TCGTTTCCCGGCCAATGCACCA98340.11308719178881652No Hit
TTATAAAGCAATGAGACTGATT98140.11285719953380571No Hit
TTCAAGTAATCCAGGATAGGCTT97320.11191423128826138No Hit
TGATGTAGTAGATTGTATAGTT92730.10663590903576323No Hit
CCCCCCACTGCTAAATTTGACTGGCT92440.10630242026599755No Hit
AATGACACGATCACTCCCGTTG92130.10594593227073079No Hit
CACTAGATTGTGAGCTCCTGGA88730.10203606393554697No Hit
TGAGGTAGTAAGTTGTATTGTT88490.10176007322953401No Hit
CAAAGAATTCTCCTTTTGGGCT87390.10049511582697453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)