FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8552377
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG130157515.2188683917933No Hit
CAACGGAATCCCAAAAGCAGCTG6880578.045213628912757No Hit
CCCCCACTGCTAAATTTGACTG5131075.999583507602623No Hit
TTCAAGTAATCCAGGATAGGCT2266642.6503041201294097No Hit
AACATTCAACGCTGTCGGTGAGT2249302.6300290550802425No Hit
AAGCTGCCAGTTGAAGAACTGT2181222.550425454818No Hit
GCCTGTCTGAGCGTCGCT2101932.457714387473798No Hit
TAGCAGCACGTAAATATTGGCG2043692.3896163604574494No Hit
AACATTCAACGCTGTCGGTGAG1389011.6241215746218858No Hit
TAGCTTATCAGACTGATGTTGAC1143941.3375696604581393No Hit
TATTGCACTTGTCCCGGCCTGTAA1051491.229471058163128No Hit
TAGCTTATCAGACTGATGTTGACT1031441.206027283409045No Hit
TGAGGTAGTAGGTTGTATAGTT919521.0751630803927377No Hit
TCCTGTCTGAGCGTCGCT889351.039886338032105No Hit
TATTGCACTTGTCCCGGCCTGTAT883661.0332332169173553No Hit
TAGCTTATCAGACTGATGTTGA785180.9180839432125127No Hit
TATTGCACTTGTCCCGGCCTGT683660.7993801021634103No Hit
TGTAAACATCCTTGACTGGAAGCT585630.684756997966764No Hit
TGAGGTAGTAGATTGTATAGTT536930.6278137645241785No Hit
TATTGCACTTGTCCCGGCCTGTA487680.5702274350160196No Hit
TTCTCACTACTGCACTTGACTAG474810.5551789870816032No Hit
AGCAGCATTGTACAGGGCTATGA460510.5384584893766962No Hit
CATAAAGTAGAAAGCACTACT395710.4626900801964179No Hit
CAACGGAATCCCAAAAGCAGCTGT373650.43689608163905774No Hit
ACTGGACTTGGAGTCAGAAGGC370600.4333298216390601No Hit
CACCCGGCTGTGTGCACATGTGC365610.4274951864259492No Hit
CTTCTCACTACTGCACTTGACTAG346800.40550130098334064No Hit
TTCAAGTAATTCAGGATAGGTT331820.3879857026882702No Hit
TGTAAACATCCCCGACTGGAAGCT319900.37404805704893507No Hit
AACCGGGCGGAAACACCA307630.35970116845878053No Hit
CCCCCACTGCTAAATTTGACTGGCTT298500.34902577377026295No Hit
CTCCCACTGCTTCACTTGACTAG290980.3402328966555146No Hit
CAACGGAATCCCAAAAGCAGCT276760.3236059401965091No Hit
CCCATAAAGTAGAAAGCACTAC274640.3211270971801173No Hit
ACCCCCCACTGCTAAATTTGACTG257160.30068833495062247No Hit
CATTGCACTTGTCTCGGTCTGA230010.2689427746227745No Hit
CCCCCCACTGCTAAATTTGACTGG223520.26135424104900895No Hit
CGCCTGTCTGAGCGTCGCT217560.25438541822934135No Hit
AACATTCAACGCTGTCGGTGAGTT193580.22634642977034336No Hit
AGCTCGGTCTGAGGCCCCTCAGT180230.21073673436051754No Hit
CCCCCCACTGCTAAATTTGACTGGCT172770.2020140131801954No Hit
TCGTACGACTCTTAGCGG170880.1998041012457706No Hit
TGAGGTAGTAGTTTGTACAGTT169980.19875176222937788No Hit
AACGGAATCCCAAAAGCAGCTG168270.19675231809823165No Hit
CCCATAAAGTAGAAAGCACT167430.19577013501626506No Hit
GACCCGCGGGCGCTCTCCAGTCCT164530.19237926485233287No Hit
TAGCTTATCAGACTGATGTTGACA152360.17814930281955532No Hit
CAAAGAATTCTCCTTTTGGGCT138210.1616041949507137No Hit
TGTAAACATCCTACACTCTCAGCT138050.16141711245891055No Hit
TAGCAGCACGTAAATATTGGC133220.1557695597376028No Hit
TATTGCACTTGTCCCGGCCTGTT133180.15572278911465198No Hit
CTCCCACTGCTTCACTTGACTAGCCT129380.15127957993432703No Hit
ACCGGGTGCTGTAGGCTTA129330.15122111665563856No Hit
TGAGGTAGTAGTTTGTGCTGTT126820.14828626006547652No Hit
TTCAAGTAATCCAGGATAGGCTT126780.14823948944252574No Hit
CTACGCCTGTCTGAGCGTCGCT124490.14556187127859307No Hit
TTCACAGTGGCTAAGTTCTGC122410.14312979888515204No Hit
ACCGGGTGCTGTAGGCTT119810.14008970839335078No Hit
CAACACCAGTCGATGGGCTGT119500.13972723606548215No Hit
TACGCCTGTCTGAGCGTCGCT117850.13779794786876212No Hit
TCGTTTCCCGGCCAATGCACCA116170.13583358170482898No Hit
CACTAGATTGTGAGCTCCTGGA112450.13148391377040558No Hit
AATGCACCCGGGCAAGGATTCT111460.13032634085237355No Hit
ACCGGGTGCTGTAGGCTTT111310.1301509510163081No Hit
TATTGCACTTGTCCCGGCCTGTAG111180.12999894649171803No Hit
CAAAGAATTCTCCTTTTGGGCTT105550.12341598131139447No Hit
CAACGGAATCCCAAAAGCAGCTGA101780.11900785009828262No Hit
TGAGGTAGTAGGTTGTGTGGTT101530.11871553370484018No Hit
CGCCCCACTGCTAAATTTGACTG99810.11670439691795625No Hit
GGAATACCGGGTGCTGTAGGCTTT98330.11497388386877706No Hit
CACTAGATTGTGAGCTCCTGGAA97990.11457633357369536No Hit
TTATAAAGCAATGAGACTGATT95750.11195717868845118No Hit
TGTAAACATCCCCGACTGGAAGC94570.1105774453114029No Hit
TTCAAGTAATCCAGGATAGGC92360.1079933683933718No Hit
CGCGACCTCAGATCAGACGTGGCG90850.10622777737697951No Hit
TTCACAGTGGCTAAGTTCTG89380.10450895698353801No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC87150.10190149475403154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)