FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10217475
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG9588249.384158023386405No Hit
CCCCCCACTGCTAAATTTGACTG7660617.497556881714905No Hit
GCCTGTCTGAGCGTCGCT4489924.394353790931713No Hit
TTCAAGTAATCCAGGATAGGCT4125304.037494586480515No Hit
TAGCAGCACGTAAATATTGGCG3524313.4492964259760854No Hit
AAGCTGCCAGTTGAAGAACTGT3494613.4202285789786617No Hit
AACATTCAACGCTGTCGGTGAGT2467472.414950856253624No Hit
CCCCCACTGCTAAATTTGACTG2292612.2438126836620595No Hit
AACATTCAACGCTGTCGGTGAG1819401.780674775323649No Hit
TGAGGTAGTAGGTTGTATAGTT1555851.522734335048532No Hit
TAGCTTATCAGACTGATGTTGAC1377391.34807278706334No Hit
TATTGCACTTGTCCCGGCCTGTAA1343021.3144343392080724No Hit
TAGCTTATCAGACTGATGTTGACT1214161.1883170744239648No Hit
TATTGCACTTGTCCCGGCCTGTAT1197081.1716006156119785No Hit
TCCTGTCTGAGCGTCGCT1137821.113601941771328No Hit
TAGCTTATCAGACTGATGTTGA989610.9685465342464749No Hit
TATTGCACTTGTCCCGGCCTGT988050.9670197382425697No Hit
TGAGGTAGTAGATTGTATAGTT850670.8325638183602112No Hit
CATAAAGTAGAAAGCACTACT732990.7173885916040901No Hit
TGTAAACATCCTTGACTGGAAGCT695950.6811369736652158No Hit
CAACGGAATCCCAAAAGCAGCTGT619500.6063141823199959No Hit
TTCAAGTAATTCAGGATAGGTT582670.5702680946124165No Hit
TATTGCACTTGTCCCGGCCTGTA514790.5038328941348034No Hit
AGCAGCATTGTACAGGGCTATGA501680.491001935409678No Hit
CCCCCACTGCTAAATTTGACTGGCTT488680.4782786353771358No Hit
ACTGGACTTGGAGTCAGAAGGC481640.4713884790518205No Hit
TCCACCACGTTCCCGTGG475110.4649974675739358No Hit
CCCATAAAGTAGAAAGCACTAC465380.4554745668572715No Hit
CACCCGGCTGTGTGCACATGTGC452640.44300573282538No Hit
GTCAGGATGGCCGAGCGGTCTAAGG422700.4137029941350481No Hit
TCGTACGACTCTTAGCGG415240.406401777347143No Hit
TTCTCACTACTGCACTTGACTAG378180.3701305851005263No Hit
CATTGCACTTGTCTCGGTCTGA362240.35452986182985524No Hit
TTCACAGTGGCTAAGTTCCGC357670.35005713251072307No Hit
TGTAAACATCCCCGACTGGAAGCT348910.3414835857195638No Hit
AACCGGGCGGAAACACCA330780.3237394757511029No Hit
CCCCCCACTGCTAAATTTGACTGGCT325100.31818037235226904No Hit
TGAGGTAGTAGTTTGTACAGTT322250.3153910334989809No Hit
CTCCCACTGCTTCACTTGACTAG317630.31086936841049284No Hit
CGCGACCTCAGATCAGACGTGG285480.27940366871462863No Hit
TGTAAACATCCTACACTCTCAGCT262550.2569617248879983No Hit
CCCCCCACTGCTAAATTTGACTGG261580.2560123709624932No Hit
CGCCTGTCTGAGCGTCGCT261320.25575790496184236No Hit
CGCGACCTCAGATCAGACGTGGCG252490.24711584809358478No Hit
CTTCTCACTACTGCACTTGACTAG252430.24705712517035763No Hit
AGCTCGGTCTGAGGCCCCTCAGT243650.23846400407145601No Hit
ACCCCCCACTGCTAAATTTGACTG238310.2332376639042425No Hit
CTACGCCTGTCTGAGCGTCGCT237000.23195554674711708No Hit
AACGGAATCCCAAAAGCAGCTG233860.22888238043156456No Hit
TGAGGTAGTAGTTTGTGCTGTT220450.21575780709030362No Hit
TAGCAGCACGTAAATATTGGC215780.2111872062324596No Hit
CCCATAAAGTAGAAAGCACT208480.20404258390649355No Hit
TAGCTTATCAGACTGATGTTGACA207940.20351407759744947No Hit
CAACACCAGTCGATGGGCTGT201630.19733838350473087No Hit
TGAGGTAGTAGGTTGTGTGGTT196840.1926503368004326No Hit
TAGCAGCACATCATGGTTTACA196720.19253289095397835No Hit
TTCAAGTAATCCAGGATAGGC178930.1751215442171378No Hit
CAAAGAATTCTCCTTTTGGGCT178620.174818142447131No Hit
TCAGGCTCAGTCCCCTCCCGAT177540.17376112982904288No Hit
TATTGCACTTGTCCCGGCCTGTT166330.16278973033944297No Hit
CACTAGATTGTGAGCTCCTGGA163310.15983400987034468No Hit
TTCACAGTGGCTAAGTTCTGC161170.1577395589419108No Hit
CTCCCACTGCTTCACTTGACTAGCCT159530.1561344657070362No Hit
AACATTCAACGCTGTCGGTGAGTT156790.15345278554633116No Hit
TATTGCACTTGTCCCGGCCTGTAG151430.14820687107137526No Hit
GCCCCCACTGCTAAATTTGACTG149210.14603412291197188No Hit
ATCACATTGCCAGGGATTTCC141220.13821418696889398No Hit
TTCAAGTAATCCAGGATAGGCTT135020.13214615156875845No Hit
TCGTTTCCCGGCCAATGCACCA132420.12960149156224998No Hit
CGCGACCTCAGATCAGACGTGGC131220.1284270330977076No Hit
CAACGGAATCCCAAAAGCAGCT130890.12810405701995847No Hit
CACTAGATTGTGAGCTCCTGGAA129170.1264206665541144No Hit
GTCAAGTAATCCAGGATAGGCT128360.1256279070905483No Hit
TCAGGCTCAGTCCCCTCCCGATA127670.12495259347343644No Hit
TGAGGTAGTAGGTTGTATAGT127300.12459046878020255No Hit
GAACGGAATCCCAAAAGCAGCTG126080.1233964360079178No Hit
TACGCCTGTCTGAGCGTCGCT125190.12252537931338223No Hit
TCAGGCTCAGTCCCCTCCCGA121400.11881604799620259No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC120840.11826796737941614No Hit
AGAGGTAGTAGGTTGCATAGTT119730.11718159329971448No Hit
TTCACAGTGGCTAAGTTCCG119120.11658457691357207No Hit
CAAAGTGCTGTTCGTGCAGGTAG118870.11633989806679244No Hit
ACCGGGTGCTGTAGGCTT114960.11251312090315856No Hit
GTCAGGATGGCCGAGTGGTCTAAGG112020.10963569766502977No Hit
CCCCCCACTGCTAAATTTGACTGT111830.10944974174147723No Hit
CAAAGTGCTGTTCGTGCAGGT110890.1085297492775857No Hit
GAGCAGCACGTAAATATTGGCG109060.1067387001191586No Hit
CAAAGAATTCTCCTTTTGGGCTT105950.10369489526521963No Hit
AGCAGCATTGTACAGGGCTATGT105190.10295107157100947No Hit
CCCCCACTGCTAAATTTGACTGGCT104760.10253022395454847No Hit
TATTGCACTTGTCCCGGCCTGTAAT103720.10151235995194507No Hit
GGAATACCGGGTGCTGTAGGCTTT103180.10098385364290101No Hit
TGTAAACATCCTACACTCAGCT102980.10078811056547729No Hit
TATTGCACTTGTCCCGGCCTGTTT102940.10074896194999254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)