FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7597240
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4651836.123052582253555No Hit
CCCCCCACTGCTAAATTTGACTG4213225.545724499949982No Hit
GCCTGTCTGAGCGTCGCT2859103.7633403709768283No Hit
AAGCTGCCAGTTGAAGAACTGT2616863.44448773501956No Hit
TTCAAGTAATCCAGGATAGGCT2379013.1314135133285244No Hit
AACATTCAACGCTGTCGGTGAGT2147122.8261842458577062No Hit
TAGCAGCACGTAAATATTGGCG2089392.7501961238555053No Hit
CCCCCACTGCTAAATTTGACTG1646532.1672739047338245No Hit
TCCTGTCTGAGCGTCGCT1358881.788649562209434No Hit
TAGCTTATCAGACTGATGTTGAC1318181.7350774755042622No Hit
AACATTCAACGCTGTCGGTGAG1248601.6434915837856907No Hit
TGAGGTAGTAGGTTGTATAGTT1162621.5303189052866566No Hit
TAGCTTATCAGACTGATGTTGACT1088271.4324544176569385No Hit
TAGCTTATCAGACTGATGTTGA929331.2232468633345794No Hit
TATTGCACTTGTCCCGGCCTGTAA773511.0181460635704547No Hit
TATTGCACTTGTCCCGGCCTGT716050.9425133337896394No Hit
CATAAAGTAGAAAGCACTACT711150.9360636231052331No Hit
TGTAAACATCCTTGACTGGAAGCT681130.896549273157094No Hit
TATTGCACTTGTCCCGGCCTGTAT678400.8929558629186389No Hit
TGAGGTAGTAGATTGTATAGTT658750.8670912068066824No Hit
CGCCTGTCTGAGCGTCGCT569160.7491668026809736No Hit
AACCGGGCGGAAACACCA526320.6927779035544487No Hit
AGCAGCATTGTACAGGGCTATGA454480.5982172473161306No Hit
ACTGGACTTGGAGTCAGAAGGC454060.5976644149717529No Hit
TTCAAGTAATTCAGGATAGGTT409060.5384323780741427No Hit
TCGTACGACTCTTAGCGG406740.5353786375051992No Hit
TATTGCACTTGTCCCGGCCTGTA381990.5028010172115137No Hit
TGTAAACATCCCCGACTGGAAGCT357160.47011809551889894No Hit
CAACGGAATCCCAAAAGCAGCTGT307900.40527875912831507No Hit
CCCATAAAGTAGAAAGCACTAC296480.39024698443118816No Hit
TTCTCACTACTGCACTTGACTAG273180.3595779519930922No Hit
TGAGGTAGTAGTTTGTACAGTT269990.35537905871079495No Hit
CACCCGGCTGTGTGCACATGTGC252500.3323575403699238No Hit
CAACACCAGTCGATGGGCTGT250910.33026467506620827No Hit
CATTGCACTTGTCTCGGTCTGA235980.31061280149106785No Hit
CGCGACCTCAGATCAGACGTGGCG232600.306163817386314No Hit
TTAATGCTAATCGTGATAGGGGTT223550.29425159663246125No Hit
CCCATAAAGTAGAAAGCACT213460.28097045769253043No Hit
CCCCCACTGCTAAATTTGACTGGCTT199150.2621346699590904No Hit
CGCGACCTCAGATCAGACGTGG191080.251512391342119No Hit
CAACGGAATCCCAAAAGCAGCT190490.25073579352501696No Hit
CTTCTCACTACTGCACTTGACTAG186300.24522063275610617No Hit
TGAGGTAGTAGTTTGTGCTGTT167000.21981667026446447No Hit
TAGCTTATCAGACTGATGTTGACA164920.21707883389230825No Hit
AACATTCAACGCTGTCGGTGAGTT163140.21473587776613615No Hit
TACGCCTGTCTGAGCGTCGCT158190.20822035370739903No Hit
ACCGGGTGCTGTAGGCTT155000.20402146042510175No Hit
TGTAAACATCCTACACTCTCAGCT154920.20391615902617266No Hit
AGCTCGGTCTGAGGCCCCTCAGT149530.19682147727332558No Hit
TTATAAAGCAATGAGACTGATT146670.1930569522616108No Hit
CTACGCCTGTCTGAGCGTCGCT142050.18697579647345614No Hit
TAGCAGCACGTAAATATTGGC140840.18538311281465375No Hit
AACGGAATCCCAAAAGCAGCTG137360.18080250196123854No Hit
CAAAGAATTCTCCTTTTGGGCT129080.16990380717207829No Hit
CCCCCCACTGCTAAATTTGACTGG127820.1682453101389452No Hit
ACGCCTGTCTGAGCGTCGCT126360.1663235596084894No Hit
CCCCCCACTGCTAAATTTGACTGGCT126140.1660339807614344No Hit
TGTCAGTTTGTCAAATACCCCAA122520.16126909245989335No Hit
TTCAAGTAATCCAGGATAGGCTT122270.16094002558823994No Hit
TTCACAGTGGCTAAGTTCTGC121400.15979487287488614No Hit
TGAGGTAGTAGGTTGTGTGGTT117750.15499049654874664No Hit
TTCACAGTGGCTAAGTTCCGC116270.15304242066855858No Hit
CTCCCACTGCTTCACTTGACTAG115560.15210787075306295No Hit
CAAAGAATTCTCCTTTTGGGCTT113910.14993602940015058No Hit
ACCGGGTGCTGTAGGCTTT112340.1478694894461673No Hit
TATTGCACTTGTCCCGGCCTGTT109930.1446972848034286No Hit
GGAATACCGGGTGCTGTAGGCTTT107250.1411696879393043No Hit
ACCCCCCACTGCTAAATTTGACTG105750.13919528670938394No Hit
TTCAAGTAATCCAGGATAGGC104630.13772106712437673No Hit
ACTGGACTTGGAGTCAGAAGGCA104560.1376289284003138No Hit
ACTGGACTTGGAGTCAGAAGGCT102690.13516750820034645No Hit
TGAGGTAGTAGGTTGTATAGT101620.13375910198966992No Hit
TTCACAGTGGCTAAGTTCCG101510.13361431256614242No Hit
ACCGGGTGCTGTAGGCTTA100480.13225855705493048No Hit
ATCCCGGACGAGCCCCCA100300.13202162890734004No Hit
CACTAGATTGTGAGCTCCTGGA99270.13066587339612806No Hit
TGATGTAGTAGGTTGTATAGTT98210.1292706298603177No Hit
TGTAAACATCCCCGACTGGAAGC96430.12692767373414557No Hit
AGCAGCATTGTACAGGGCTATGT95700.12596679846891767No Hit
TGTCAGTTTGTCAAATACCCCA95150.12524285135128022No Hit
AATGCACCCGGGCAAGGATTCT94940.12496643517909137No Hit
TGAGAACTGAATTCCATGGGTT94250.124058210613328No Hit
CGCGACCTCAGATCAGACGTGGC90580.11922750893745622No Hit
AGAGGTAGTAGGTTGCATAGTT88260.11617376836851277No Hit
ACTGGACTTGGAGTCAGAAGG87580.11527870647761555No Hit
TATTGCACTTGTCCCGGCCTGTAG86330.1136333721193486No Hit
TAGCAGCACGTAAATATTGGCGT85810.11294891302630955No Hit
TCGTTTCCCGGCCAATGCACCA84990.11186957368728645No Hit
ACCACAGGGTAGAACCACGGAC84640.1114088800669717No Hit
GGAATACCGGGTGCTGTAGGCTT83830.1103427034028147No Hit
CAAAGTGCTGTTCGTGCAGGT82680.10882899579320912No Hit
CCCTGTCTGAGCGTCGCT81420.10717049876007602No Hit
AGCAGCATTGTACAGGGCTATG79340.10443266238791982No Hit
TGAGGTAGTAAGTTGTATTGTT79000.10398513144247122No Hit
CACTAGATTGTGAGCTCCTGGAA77080.10145789786817318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)