FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9912518
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7758607.827072798253683No Hit
CAACGGAATCCCAAAAGCAGCTG5215545.2615692602021005No Hit
AAGCTGCCAGTTGAAGAACTGT3996644.031911972316217No Hit
TTCAAGTAATCCAGGATAGGCT2843162.868252042518359No Hit
AACATTCAACGCTGTCGGTGAGT2841402.8664765098030593No Hit
TAGCAGCACGTAAATATTGGCG2710892.7348147060111265No Hit
CCCCCACTGCTAAATTTGACTG2325912.346437101047383No Hit
TAGCTTATCAGACTGATGTTGAC2262392.282356511231556No Hit
GCCTGTCTGAGCGTCGCT1921291.9382461651015412No Hit
TAGCTTATCAGACTGATGTTGACT1737701.7530359087368115No Hit
TATTGCACTTGTCCCGGCCTGTAA1541601.5552052465377617No Hit
AACATTCAACGCTGTCGGTGAG1478101.4911448332300632No Hit
TATTGCACTTGTCCCGGCCTGTAT1457911.4707766482744344No Hit
TGAGGTAGTAGGTTGTATAGTT1237701.2486232055265878No Hit
TAGCTTATCAGACTGATGTTGA1200641.211236135964646No Hit
TGTAAACATCCTTGACTGGAAGCT1165991.1762803356321774No Hit
TATTGCACTTGTCCCGGCCTGT1140381.1504443169737497No Hit
AGCAGCATTGTACAGGGCTATGA829450.8367702333554401No Hit
TGAGGTAGTAGATTGTATAGTT798390.8054361162320209No Hit
AACCGGGCGGAAACACCA737190.7436960013590896No Hit
TATTGCACTTGTCCCGGCCTGTA711730.718011306511625No Hit
ACTGGACTTGGAGTCAGAAGGC645980.6516810360394806No Hit
TTAATGCTAATCGTGATAGGGGTT644060.6497440912591533No Hit
CATAAAGTAGAAAGCACTACT605800.611146431209507No Hit
CGCCTGTCTGAGCGTCGCT603720.6090480743641524No Hit
TGTAAACATCCCCGACTGGAAGCT588620.5938148107272038No Hit
TTCAAGTAATTCAGGATAGGTT568960.5739813032369777No Hit
CCCATAAAGTAGAAAGCACTAC404460.4080295238808141No Hit
CAACGGAATCCCAAAAGCAGCTGT373370.37666514199520246No Hit
TTCTCACTACTGCACTTGACTAG369760.3730232822780246No Hit
TGTAAACATCCTACACTCTCAGCT369730.37299301751583197No Hit
TCCTGTCTGAGCGTCGCT363170.36637512284971385No Hit
CACCCGGCTGTGTGCACATGTGC318140.3209477147986011No Hit
TGAGGTAGTAGTTTGTACAGTT308840.31156563851889096No Hit
CTTCTCACTACTGCACTTGACTAG308350.311071314069745No Hit
GGAATACCGGGTGCTGTAGGCTTT303830.30651142323272457No Hit
TAGCTTATCAGACTGATGTTGACA302340.305008273377158No Hit
CTACGCCTGTCTGAGCGTCGCT301160.30381785939758194No Hit
TCGTACGACTCTTAGCGG290860.29342695771145133No Hit
AACATTCAACGCTGTCGGTGAGTT280730.2832075563444122No Hit
TACGCCTGTCTGAGCGTCGCT278960.281421935375048No Hit
CATTGCACTTGTCTCGGTCTGA276390.2788292540805475No Hit
CCCCCACTGCTAAATTTGACTGGCTT275290.27771954613348493No Hit
AGCTCGGTCTGAGGCCCCTCAGT260930.2632328132972873No Hit
CCCCCCACTGCTAAATTTGACTGG229580.23160613680600634No Hit
CCCATAAAGTAGAAAGCACT216160.21806769985184388No Hit
GGAATACCGGGTGCTGTAGGCTT207500.20933127183224284No Hit
CCCCCCACTGCTAAATTTGACTGGCT206660.20848385849084966No Hit
CAAAGAATTCTCCTTTTGGGCT206410.20823165213924455No Hit
TATTGCACTTGTCCCGGCCTGTT203050.20484199877367185No Hit
TGAGGTAGTAGTTTGTGCTGTT201560.20333884891810539No Hit
TTCAAGTAATCCAGGATAGGCTT199750.20151287493248438No Hit
CTCCCACTGCTTCACTTGACTAG197500.19924301776803832No Hit
ACGCCTGTCTGAGCGTCGCT193130.194834450741981No Hit
CAAAGAATTCTCCTTTTGGGCTT190970.19265538786411282No Hit
TATTGCACTTGTCCCGGCCTGTAG181940.1835456944441362No Hit
AGCAGCATTGTACAGGGCTATGT176320.17787609566005327No Hit
ACCGGGTGCTGTAGGCTT174640.17618126897726694No Hit
CAACACCAGTCGATGGGCTGT170630.17213587909752093No Hit
GGGCTACGCCTGTCTGAGCGTCGCT168750.17023928733345048No Hit
CGCGACCTCAGATCAGACGTGGCG167910.16939187399205732No Hit
TTATAAAGCAATGAGACTGATT165540.16700095777884086No Hit
ACCCCCCACTGCTAAATTTGACTG165050.16650663332969484No Hit
CACTAGATTGTGAGCTCCTGGA164620.16607283840493406No Hit
TGTAAACATCCCCGACTGGAAGC160160.16157347709229883No Hit
CGCGACCTCAGATCAGACGTGG158490.1598887386635767No Hit
GCGTTCGCGCTTTCCCCTG158020.1594145907225591No Hit
ACTGGACTTGGAGTCAGAAGGCA157920.15931370818191704No Hit
CAACGGAATCCCAAAAGCAGCT151120.152453695418258No Hit
CACTAGATTGTGAGCTCCTGGAA148160.14946757221525347No Hit
CAAAGTGCTGTTCGTGCAGGTAG145990.14727842108332112No Hit
TGTAAACATCCTACACTCAGCT145770.1470564794939086No Hit
TGATGTAGTAGGTTGTATAGTT144210.14548271185989273No Hit
ACCGGGTGCTGTAGGCTTT143450.1447160045510132No Hit
ATCCCGGACGAGCCCCCA142250.14350541406330863No Hit
ACCGGGTGCTGTAGGCTTA141410.14265800072191545No Hit
TAGCAGCACGTAAATATTGGC140660.14190138166710012No Hit
TATTGCACTTGTCCCGGCCTGTTT137080.13828978671211492No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT135820.13701866670002516No Hit
TGAGGTAGTAGGTTGTGTGGTT132890.13406280825921324No Hit
TTCACAGTGGCTAAGTTCCG129400.1305420075908059No Hit
AACGGAATCCCAAAAGCAGCTG127610.1287362101133133No Hit
ACCACAGGGTAGAACCACGGAC126670.12778791423127808No Hit
TTCAAGTAATCCAGGATAGGC126210.12732385454432465No Hit
CCAAGTCCTTCTGATCGAGGCCC123530.12462020245511786No Hit
GGGAATACCGGGTGCTGTAGGCTTT122280.1233591706970923No Hit
TGTCAGTTTGTCAAATACCCCA121980.12305652307516618No Hit
ACTGGACTTGGAGTCAGAAGG121750.12282449323168945No Hit
AGCAGCATTGTACAGGGCTATG120580.12164416750617753No Hit
TGAGAACTGAATTCCATAGGCTGT118960.12000987034777642No Hit
TAGCAGCACGTAAATATTGGCGT113430.11443106585027135No Hit
TATTGCACTTGTCCCGGCCTGTAAT113420.11442097759620715No Hit
ACTGGACTTGGAGTCAGAAGGCT111990.11297835726502589No Hit
TGTCAGTTTGTCAAATACCCCAA111160.11214103217769691No Hit
GTCAGGATGGCCGAGCGGTCTAAGG110610.11158617820416568No Hit
TGAGGTAGTAGGTTGTATAGT109950.11092035343592818No Hit
TTCACAGTGGCTAAGTTCCGC109860.11082955914935035No Hit
TTCACAGTGGCTAAGTTCTGC108620.10957861564538898No Hit
TTAATGCTAATCGTGATAGGGGTTT108340.10929614453159128No Hit
GGGAATACCGGGTGCTGTAGGCTT107050.10799475975730888No Hit
CCCCCCACTGCTAAATTTGACTGT106790.10773246515163956No Hit
CAAAGTGCTGTTCGTGCAGGT103490.1044033413104521No Hit
TGAGAACTGAATTCCATGGGTT102400.1033037216174538No Hit
ATCACATTGCCAGGGATTTCCA102300.10320283907681177No Hit
GAATACCGGGTGCTGTAGGCTTT101670.10256727907076689No Hit
CTCCCACTGCTTCACTTGACTAGCCT101130.10202251335129985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)