FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6296608
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG80191512.735666568412707No Hit
CCCCCCACTGCTAAATTTGACTG76599012.165121284348652No Hit
GCCTGTCTGAGCGTCGCT6231589.89672534799689No Hit
CCCCCACTGCTAAATTTGACTG2624954.168831853594825No Hit
TTCAAGTAATCCAGGATAGGCT2217703.5220550493217937No Hit
TCCTGTCTGAGCGTCGCT1786952.8379565632797847No Hit
TAGCAGCACGTAAATATTGGCG1519462.413140535348556No Hit
AACATTCAACGCTGTCGGTGAGT1056081.6772204971311537No Hit
AAGCTGCCAGTTGAAGAACTGT846351.3441363985180592No Hit
AACATTCAACGCTGTCGGTGAG790901.2560731111099817No Hit
AACCGGGCGGAAACACCA564280.8961650463233538No Hit
TAGCTTATCAGACTGATGTTGAC421780.6698527207029563No Hit
TAGCTTATCAGACTGATGTTGA399820.6349768002073497No Hit
CAACGGAATCCCAAAAGCAGCTGT378040.6003867479125269No Hit
CCCCCACTGCTAAATTTGACTGGCTT368440.5851404438707316No Hit
CATAAAGTAGAAAGCACTACT357660.5680201149571326No Hit
TGAGGTAGTAGGTTGTATAGTT320450.5089248052284658No Hit
TAGCTTATCAGACTGATGTTGACT314050.4987606025339358No Hit
CCCATAAAGTAGAAAGCACTAC302610.4805920902174631No Hit
CGCCTGTCTGAGCGTCGCT295860.46987203268807587No Hit
CCCTGTCTGAGCGTCGCT291080.4622806438005987No Hit
TATTGCACTTGTCCCGGCCTGTAA239770.3807923250105454No Hit
CTACGCCTGTCTGAGCGTCGCT228490.36287791776143596No Hit
AGCAGCATTGTACAGGGCTATGA228270.3625285232938115No Hit
TCGTACGACTCTTAGCGG208780.33157534977562525No Hit
TTCAAGTAATTCAGGATAGGTT208190.33063833733972325No Hit
CATTGCACTTGTCTCGGTCTGA208030.33038423227235997No Hit
TTAATGCTAATCGTGATAGGGGTT199470.31678961116842597No Hit
CAACACCAGTCGATGGGCTGT192830.3062442508728509No Hit
CCCCCCACTGCTAAATTTGACTGG192250.3053231200036591No Hit
TTCTCACTACTGCACTTGACTAG190320.3022579776285899No Hit
CACCCGGCTGTGTGCACATGTGC185670.29487304910834533No Hit
CAACGGAATCCCAAAAGCAGCT177510.2819136906728194No Hit
TATTGCACTTGTCCCGGCCTGTAT175730.2790867717984032No Hit
ACTGGACTTGGAGTCAGAAGGC174630.27733979946028087No Hit
TATTGCACTTGTCCCGGCCTGT154050.2446555351706824No Hit
ACCCCCCACTGCTAAATTTGACTG141180.22421595881465067No Hit
CTTCTCACTACTGCACTTGACTAG140210.22267544684376095No Hit
TGAGGTAGTAGATTGTATAGTT139350.2213096321066835No Hit
CCCATAAAGTAGAAAGCACT134510.21362295381894503No Hit
TGTAAACATCCTACACTCTCAGCT133710.2123524284821288No Hit
CCCCCCACTGCTAAATTTGACTGGCT126840.2014417921522191No Hit
AACGGAATCCCAAAAGCAGCTG124500.19772550554203153No Hit
CAAAGAATTCTCCTTTTGGGCT124110.19710612444033357No Hit
TGTAAACATCCTTGACTGGAAGCT120130.19078526088967268No Hit
CTCCCACTGCTTCACTTGACTAG115590.18357502960324035No Hit
ACGCCTGTCTGAGCGTCGCT109500.17390315547672652No Hit
TTCACAGTGGCTAAGTTCCG108560.1724102882059674No Hit
CGCGACCTCAGATCAGACGTGG108090.17166385457058783No Hit
TGATGTAGTAGGTTGTATAGTT102030.16203962514420464No Hit
TTCACAGTGGCTAAGTTCCGC101600.16135671777566588No Hit
TACGCCTGTCTGAGCGTCGCT99560.15811687816678438No Hit
ACCGGGTGCTGTAGGCTT98560.15652872149576408No Hit
TATTGCACTTGTCCCGGCCTGTA98480.15640166896208246No Hit
ACCTGTCTGAGCGTCGCT92980.1476668072714706No Hit
TAGCAGCACGTAAATATTGGC91780.1457610192662462No Hit
AACATTCAACGCTGTCGGTGAGTT89940.14283881099156878No Hit
ATCACATTGCCAGGGATTTCC88560.14064715478556072No Hit
AGCTCGGTCTGAGGCCCCTCAGT87430.13885253774730777No Hit
TTCAAGTAATCCAGGATAGGC78050.12395562817313704No Hit
GCCGGTCTGAGCGTCGCT73800.11720596232130062No Hit
TGAGAACTGAATTCCATGGGTT72440.11504606924871295No Hit
ATCCCGGACGAGCCCCCA72060.11444256971372524No Hit
CCCATAAAGTAGAAAGCACTA69270.1100116126015785No Hit
CACTAGATTGTGAGCTCCTGGA67200.1067241282925664No Hit
CACCCGTAGAACCGACCTTGCG66260.1052312610218073No Hit
ACCGGGTGCTGTAGGCTTT65610.10419895918564406No Hit
TCGTTTCCCGGCCAATGCACCA65370.10381780158459919No Hit
TAGCAGCACATCATGGTTTACA64830.10296019698224823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)