FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7922074
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6521978.232654731576606No Hit
CAACGGAATCCCAAAAGCAGCTG5989247.56019193963601No Hit
GCCTGTCTGAGCGTCGCT5652647.135303204691095No Hit
TTCAAGTAATCCAGGATAGGCT2831243.5738620972235298No Hit
CCCCCACTGCTAAATTTGACTG2582383.25972718760264No Hit
AAGCTGCCAGTTGAAGAACTGT2264572.858556988990509No Hit
TAGCAGCACGTAAATATTGGCG2197982.7745007178675682No Hit
AACATTCAACGCTGTCGGTGAGT1656122.0905131661229115No Hit
AACATTCAACGCTGTCGGTGAG1357041.7129857661011498No Hit
TGAGGTAGTAGGTTGTATAGTT1167411.473616631200365No Hit
CATAAAGTAGAAAGCACTACT904861.1422008933519179No Hit
TAGCTTATCAGACTGATGTTGA839901.0602021642312354No Hit
TAGCTTATCAGACTGATGTTGAC824231.0404219905040017No Hit
TATTGCACTTGTCCCGGCCTGT821521.0370011691382837No Hit
TATTGCACTTGTCCCGGCCTGTAA802011.012373779896527No Hit
TAGCTTATCAGACTGATGTTGACT788180.9949162302700025No Hit
TATTGCACTTGTCCCGGCCTGTAT742900.937759480661251No Hit
TGAGGTAGTAGATTGTATAGTT639820.8076420391932718No Hit
AACCGGGCGGAAACACCA613940.7749738262985173No Hit
TCGTACGACTCTTAGCGG610520.7706567749808951No Hit
TGTAAACATCCTTGACTGGAAGCT519180.6553586851120048No Hit
TTCAAGTAATTCAGGATAGGTT473880.5981766895891152No Hit
TATTGCACTTGTCCCGGCCTGTA449450.5673388054693758No Hit
CCCATAAAGTAGAAAGCACTAC414780.5235750133109082No Hit
CGCCTGTCTGAGCGTCGCT375840.47442121848394747No Hit
AGCAGCATTGTACAGGGCTATGA355580.4488471074620106No Hit
TTCTCACTACTGCACTTGACTAG354320.44725661487130774No Hit
CAACGGAATCCCAAAAGCAGCTGT341230.4307331640678943No Hit
CACCCGGCTGTGTGCACATGTGC326770.4124803681460183No Hit
ACTGGACTTGGAGTCAGAAGGC321220.4054746269726842No Hit
CCCCCACTGCTAAATTTGACTGGCTT300980.37992576186488536No Hit
CAACACCAGTCGATGGGCTGT286990.3622662449252557No Hit
CATTGCACTTGTCTCGGTCTGA282910.3571160784410749No Hit
TGTAAACATCCCCGACTGGAAGCT252850.3191714694914488No Hit
CCCATAAAGTAGAAAGCACT251560.3175431080295387No Hit
ACCGGGTGCTGTAGGCTT249220.3145893360753762No Hit
TGAGGTAGTAGTTTGTACAGTT237660.29999719770353067No Hit
CTCCCACTGCTTCACTTGACTAG232450.2934206370705449No Hit
CTTCTCACTACTGCACTTGACTAG196060.2474856962961972No Hit
ACCGGGTGCTGTAGGCTTA167600.2115607604776224No Hit
TGATGTAGTAGGTTGTATAGTT167450.21137141612158636No Hit
AACGGAATCCCAAAAGCAGCTG164030.20705436480396422No Hit
TTCAAGTAATCCAGGATAGGC160910.20311600219841422No Hit
ACCGGGTGCTGTAGGCTTT160860.20305288741306887No Hit
TAGCAGCACGTAAATATTGGC157370.19864747539596322No Hit
TTCACAGTGGCTAAGTTCCG155980.19689288436336244No Hit
GGAATACCGGGTGCTGTAGGCTTT152640.19267681670229286No Hit
ACCCCCCACTGCTAAATTTGACTG151230.19089697975555392No Hit
AGCTCGGTCTGAGGCCCCTCAGT150380.1898240284046829No Hit
CTACGCCTGTCTGAGCGTCGCT149500.1887132081826047No Hit
GTCAGGATGGCCGAGCGGTCTAAGG146700.18517878020326498No Hit
TTCACAGTGGCTAAGTTCCGC145850.18410582885239396No Hit
CAACGGAATCCCAAAAGCAGCT144290.18213664754961895No Hit
ATCCCGGACGAGCCCCCA143340.18093746662805724No Hit
CCCCCCACTGCTAAATTTGACTGGCT143010.1805209090447779No Hit
TGAGGTAGTAGTTTGTGCTGTT142700.18012959737563672No Hit
CCCCCCACTGCTAAATTTGACTGG142440.1798014004918409No Hit
TGTAAACATCCTACACTCTCAGCT134750.17009434650572564No Hit
TATTGCACTTGTCCCGGCCTGTT134470.16974090370779166No Hit
GGAATACCGGGTGCTGTAGGCTT131380.16584040997344887No Hit
TTCACAGTGGCTAAGTTCTGC124270.15686548749733972No Hit
TGAGGTAGTAGGTTGTGTGGTT120090.1515890914424682No Hit
TACGCCTGTCTGAGCGTCGCT114810.14492417010999897No Hit
TGAGGTAGTAGGTTGTATAGT112130.14154121761548807No Hit
AACATTCAACGCTGTCGGTGAGTT111620.14089744680496546No Hit
CAAAGAATTCTCCTTTTGGGCT108660.1371610515125206No Hit
TTCAAGTAATCCAGGATAGGCTT107070.1351540013385384No Hit
TAGCTTATCAGACTGATGTTGACA107050.13512875542440023No Hit
TTCACAGTGGCTAAGTTCTG106140.1339800663311148No Hit
TTATAAAGCAATGAGACTGATT106130.13396744337404573No Hit
ACGCCTGTCTGAGCGTCGCT101900.12862793253382887No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC101840.12855219479141447No Hit
CACTAGATTGTGAGCTCCTGGA97500.12307383142343783No Hit
CCCATAAAGTAGAAAGCACTA94290.1190218622042662No Hit
TGATGTAGTAGATTGTATAGTT93860.11847907505029617No Hit
AGAGGTAGTAGGTTGCATAGTT90980.11484366341440386No Hit
CTCCCACTGCTTCACTTGACTAGCCT90900.1147426797578513No Hit
CAAAGTGCTGTTCGTGCAGGT90310.11399792529077613No Hit
TCGTTTCCCGGCCAATGCACCA87140.10999644789988075No Hit
TATTGCACTTGTCCCGGCCTGTAG86790.1095546444024633No Hit
AGCAGCATTGTACAGGGCTATGT84880.10714365960227082No Hit
AGAATTACTCTGAGACCT83660.10560365883984421No Hit
CGCGACCTCAGATCAGACGTGG83000.10477054367328555No Hit
ATCACATTGCCAGGGATTTCC82190.10374808415069084No Hit
TCCCACTGCTTCACTTGACTAG81030.10228382113067866No Hit
TGTAAACATCCCCGACTGGAAGC79970.10094578768135719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)