FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6240218
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG72068011.548955501234092No Hit
CAACGGAATCCCAAAAGCAGCTG3329155.334989899391336No Hit
CCCCCACTGCTAAATTTGACTG2410383.862653516271387No Hit
GCCTGTCTGAGCGTCGCT1634952.620020646714586No Hit
AACATTCAACGCTGTCGGTGAGT1532282.455491138290361No Hit
TTCAAGTAATCCAGGATAGGCT1470592.3566324125214857No Hit
AAGCTGCCAGTTGAAGAACTGT1330482.1321050001778783No Hit
TAGCAGCACGTAAATATTGGCG1109651.778223132589278No Hit
CGCCTGTCTGAGCGTCGCT956421.532670813744007No Hit
AACATTCAACGCTGTCGGTGAG937081.5016783067514627No Hit
TGAGGTAGTAGGTTGTATAGTT636091.0193393884636721No Hit
ACCGGGTGCTGTAGGCTT557210.8929335481548882No Hit
TAGCTTATCAGACTGATGTTGAC508810.8153721552676525No Hit
TATTGCACTTGTCCCGGCCTGT471050.7548614487506686No Hit
TAGCTTATCAGACTGATGTTGA455940.7306475510951701No Hit
TAGCTTATCAGACTGATGTTGACT437740.7014819033565814No Hit
ACCGGGTGCTGTAGGCTTT435660.6981486864721713No Hit
TCGTACGACTCTTAGCGG410060.6571244786640467No Hit
TATTGCACTTGTCCCGGCCTGTAT372060.5962291701988617No Hit
TATTGCACTTGTCCCGGCCTGTAA364430.5840020332622995No Hit
CATAAAGTAGAAAGCACTACT355490.569675610691806No Hit
AACCGGGCGGAAACACCA339430.5439393303246778No Hit
ACCGGGTGCTGTAGGCTTA335950.5383626020757608No Hit
TGAGGTAGTAGATTGTATAGTT333230.5340037799961476No Hit
TCCTGTCTGAGCGTCGCT318510.5104148605064759No Hit
ACTGGACTTGGAGTCAGAAGGC279580.44802921949201135No Hit
TATTGCACTTGTCCCGGCCTGTA250770.4018609606266961No Hit
AGCAGCATTGTACAGGGCTATGA230020.36860891718847No Hit
TGTAAACATCCTTGACTGGAAGCT229760.36819226507791875No Hit
TTCAAGTAATTCAGGATAGGTT211300.33860996522877884No Hit
CAACGGAATCCCAAAAGCAGCTGT202660.3247642950935368No Hit
TACGCCTGTCTGAGCGTCGCT183890.2946852177279704No Hit
CAACACCAGTCGATGGGCTGT183580.2941884402115439No Hit
CACCCGGCTGTGTGCACATGTGC174850.2801985443457264No Hit
ACGCCTGTCTGAGCGTCGCT173970.2787883372023221No Hit
ATCCCGGACGAGCCCCCA168330.26975019141959466No Hit
TTCTCACTACTGCACTTGACTAG150660.2414338729832836No Hit
TGTAAACATCCCCGACTGGAAGCT145080.23249187768760643No Hit
CATTGCACTTGTCTCGGTCTGA140220.22470368823653275No Hit
TGAGGTAGTAGTTTGTACAGTT138810.2224441517908509No Hit
CTACGCCTGTCTGAGCGTCGCT137290.2200083394522435No Hit
CCCATAAAGTAGAAAGCACT134120.2149283887197531No Hit
CCCATAAAGTAGAAAGCACTAC133870.21452776169037685No Hit
TTAATGCTAATCGTGATAGGGGTT129300.2072042995933796No Hit
CAACGGAATCCCAAAAGCAGCT126600.20287752767611644No Hit
TGAGGTAGTAGGTTGTGTGGTT111800.17916040753704437No Hit
CCCCCCACTGCTAAATTTGACTGG111460.1786155547770927No Hit
AACGGAATCCCAAAAGCAGCTG107430.1721574470635481No Hit
GCGTTCGCGCTTTCCCCTG104600.16762234909100934No Hit
AGCTCGGTCTGAGGCCCCTCAGT98000.15704579551547718No Hit
ACCCCCCACTGCTAAATTTGACTG95290.152702998517039No Hit
CGCGACCTCAGATCAGACGTGG95020.15227032132531268No Hit
GACCCGCGGGCGCTCTCCAGTCCT94670.15170944348418597No Hit
AACATTCAACGCTGTCGGTGAGTT94640.15166136824066082No Hit
CTTCTCACTACTGCACTTGACTAG91280.14627694096584445No Hit
TAGTGGGTTATCAGAACT90220.1445782823612893No Hit
TGAGGTAGTAGTTTGTGCTGTT87210.13975473292759966No Hit
ATCCCACCGCTGCCACCA78650.12603726344175797No Hit
CCCCCCACTGCTAAATTTGACTGTA76760.12300852309967376No Hit
CCCCCCACTGCTAAATTTGACTGT76660.12284827228792328No Hit
CGCGACCTCAGATCAGACGTGGCG75300.12066886124811665No Hit
TTCAAGTAATCCAGGATAGGC74720.11973940653996383No Hit
CTCCCACTGCTTCACTTGACTAG73410.11764012090603244No Hit
TTCAAGTAATCCAGGATAGGCTT72950.1169029671719802No Hit
TAGCAGCACGTAAATATTGGC71970.11533250921682543No Hit
TGAGGTAGTAGGTTGTATAGT71670.11485175678157397No Hit
TATTGCACTTGTCCCGGCCTGTT71300.11425882877809718No Hit
CCACCCTGAACGCGCCCGA70510.11299284736526834No Hit
GGAATACCGGGTGCTGTAGGCTTT69800.11185506660183987No Hit
TCCACCACGTTCCCGTGG69230.1109416369748621No Hit
TTCACAGTGGCTAAGTTCCG68130.10917887804560673No Hit
CCCCCACTGCTAAATTTGACTGGCTT66930.1072558683046009No Hit
ACCACAGGGTAGAACCACGGAC66400.10640653900232332No Hit
GGAATACCGGGTGCTGTAGGCTT65130.10437135369309213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)