FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9420146
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG160725517.0618905481932No Hit
CAACGGAATCCCAAAAGCAGCTG4540894.820402995877134No Hit
CCCCCACTGCTAAATTTGACTG3800574.0345128408837825No Hit
GCCTGTCTGAGCGTCGCT2651412.814616673669389No Hit
AACATTCAACGCTGTCGGTGAGT2536992.693153588065408No Hit
AAGCTGCCAGTTGAAGAACTGT2400682.548453070684892No Hit
TAGCAGCACGTAAATATTGGCG2120722.251260224629215No Hit
TTCAAGTAATCCAGGATAGGCT2055022.181516082659441No Hit
TAGCTTATCAGACTGATGTTGAC1501061.5934572563949645No Hit
TAGCTTATCAGACTGATGTTGACT1169831.2418385022907288No Hit
AACATTCAACGCTGTCGGTGAG1143911.2143230051848455No Hit
TAGCTTATCAGACTGATGTTGA970001.0297080321260415No Hit
TATTGCACTTGTCCCGGCCTGTAT956391.015260273036108No Hit
TATTGCACTTGTCCCGGCCTGTAA872710.9264293780584717No Hit
TATTGCACTTGTCCCGGCCTGT803240.8526831749741458No Hit
TGAGGTAGTAGGTTGTATAGTT710030.7537356639695393No Hit
TGTAAACATCCTTGACTGGAAGCT620480.6586734430655321No Hit
TCGTACGACTCTTAGCGG556860.5911373348141313No Hit
TTAATGCTAATCGTGATAGGGGTT546300.5799273174746973No Hit
AACCGGGCGGAAACACCA536300.5693117707517484No Hit
AGCAGCATTGTACAGGGCTATGA519080.5510317992948305No Hit
ACTGGACTTGGAGTCAGAAGGC500630.5314461155909899No Hit
TATTGCACTTGTCCCGGCCTGTA493150.5235056866422241No Hit
TTCAAGTAATTCAGGATAGGTT479070.508558996856312No Hit
TGAGGTAGTAGATTGTATAGTT447790.4753535667069279No Hit
CGCCTGTCTGAGCGTCGCT386670.4104713451362644No Hit
CATAAAGTAGAAAGCACTACT362420.3847286443331133No Hit
TGTAAACATCCCCGACTGGAAGCT350310.3718732172516222No Hit
CAACGGAATCCCAAAAGCAGCTGT317240.33676760423883023No Hit
ACCGGGTGCTGTAGGCTT292120.31010135087078267No Hit
GGAATACCGGGTGCTGTAGGCTTT271280.2879785515001572No Hit
TGTAAACATCCTACACTCTCAGCT268980.285536975753879No Hit
ACCGGGTGCTGTAGGCTTT264930.2812376793310847No Hit
GACCCGCGGGCGCTCTCCAGTCCT258690.2746135781759646No Hit
CCCATAAAGTAGAAAGCACTAC257040.272862012966678No Hit
TTCTCACTACTGCACTTGACTAG246140.2612910670386637No Hit
ACCGGGTGCTGTAGGCTTA237960.2526075498192915No Hit
CATTGCACTTGTCTCGGTCTGA236130.2506649047689919No Hit
CTTCTCACTACTGCACTTGACTAG226930.24089860178387892No Hit
CCCCCCACTGCTAAATTTGACTGG215270.22852087430492055No Hit
AACATTCAACGCTGTCGGTGAGTT212270.22533621028803588No Hit
GGGCTACGCCTGTCTGAGCGTCGCT212170.2252300548208064No Hit
CTACGCCTGTCTGAGCGTCGCT210290.22323433203689202No Hit
ACCCCCCACTGCTAAATTTGACTG201500.21390326646741992No Hit
GGAATACCGGGTGCTGTAGGCTT196160.20823456451736522No Hit
CCCCCCACTGCTAAATTTGACTGT193650.20557006228990507No Hit
CACCCGGCTGTGTGCACATGTGC192560.20441296769710365No Hit
TACGCCTGTCTGAGCGTCGCT182490.19372311214709412No Hit
TAGCTTATCAGACTGATGTTGACA181820.19301187051665653No Hit
CCCCCCACTGCTAAATTTGACTGTA174820.18558098781059232No Hit
CTCCCACTGCTTCACTTGACTAG172520.18313941206431408No Hit
TTCAAGTAATCCAGGATAGGCTT162320.17231155440690624No Hit
CCCCCCACTGCTAAATTTGACTGA161620.17156846613629978No Hit
CCCCCCACTGCTAAATTTGACTGGCT161030.17094214887964582No Hit
AGCTCGGTCTGAGGCCCCTCAGT160280.17014598287542465No Hit
TGAGGTAGTAGTTTGTACAGTT155200.1647532851401666No Hit
CAAAGAATTCTCCTTTTGGGCT154460.1639677346826684No Hit
CAACGGAATCCCAAAAGCAGCT151090.16039029543703465No Hit
CACTAGATTGTGAGCTCCTGGA149220.1584051881998432No Hit
CCCCCACTGCTAAATTTGACTGGCTT148630.1577788709431892No Hit
TATTGCACTTGTCCCGGCCTGTT144270.1531504925719835No Hit
GCGTTCGCGCTTTCCCCTG142770.15155816056354116No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT138660.14719517086040917No Hit
TGAGGTAGTAGTTTGTGCTGTT137950.14644146704307978No Hit
CCCATAAAGTAGAAAGCACT135310.1436389627082213No Hit
CCCCCACAACCGCGCTTGACTAG126720.1345202080732082No Hit
CAAAGAATTCTCCTTTTGGGCTT122110.12962644103392879No Hit
AACTTAACTTGACCGCTCTGACCA119940.12732286739504886No Hit
AGCAGCATTGTACAGGGCTATGT119500.1268557833392391No Hit
CACTAGATTGTGAGCTCCTGGAA117830.12508298703650664No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC115010.12208940286063508No Hit
ACGCCTGTCTGAGCGTCGCT112850.11979644476847812No Hit
GAATACCGGGTGCTGTAGGCTTT112730.11966905820780273No Hit
AACGGAATCCCAAAAGCAGCTG108900.1156033038129133No Hit
TATTGCACTTGTCCCGGCCTGTAG108140.1147965222619692No Hit
GTCCGATGGTAGTGGGTTATCAGAAC107930.11457359578078727No Hit
GGGAATACCGGGTGCTGTAGGCTTT105550.11204709566072543No Hit
TTCAAGTAATCCAGGATAGGC103130.10947813335377181No Hit
TGAGGTAGTAGGTTGTGTGGTT101230.1074611794764115No Hit
CAAAGTGCTGTTCGTGCAGGTAG99260.10536991677199059No Hit
TAGCAGCACGTAAATATTGGC99080.10517883693097749No Hit
CAACACCAGTCGATGGGCTGT96470.10240817923628785No Hit
CTCCCACTGCTTCACTTGACTAGCCT96170.10208971283459937No Hit
TTCACAGTGGCTAAGTTCCG95460.10133600901727001No Hit
GTGCGGTAACGCGACCGATCCCGGA94420.10023199215808333No Hit
TGTAAACATCCCCGACTGGAAGC94360.10016829887774563No Hit
AGCAGCATTGTACAGGGCTATG94290.10009399005068498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)