FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9010548
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG107575211.93880771735526No Hit
GCCTGTCTGAGCGTCGCT90369110.029256822115592No Hit
CAACGGAATCCCAAAAGCAGCTG5659946.281460350691212No Hit
CCCCCACTGCTAAATTTGACTG4163564.620762244427309No Hit
TTCAAGTAATCCAGGATAGGCT2715753.013967629937713No Hit
TAGCAGCACGTAAATATTGGCG2060192.2864203153903624No Hit
AAGCTGCCAGTTGAAGAACTGT1902602.1115252923573573No Hit
AACATTCAACGCTGTCGGTGAGT1493391.6573797731281161No Hit
AACATTCAACGCTGTCGGTGAG1295571.4378370771677815No Hit
TGAGGTAGTAGGTTGTATAGTT990421.0991784295472373No Hit
CATAAAGTAGAAAGCACTACT901861.0008936193447946No Hit
TAGCTTATCAGACTGATGTTGA825970.9166701070789479No Hit
AACCGGGCGGAAACACCA661500.7341395884023925No Hit
TAGCTTATCAGACTGATGTTGAC645940.71687093837134No Hit
TATTGCACTTGTCCCGGCCTGT642820.7134083298818229No Hit
TAGCTTATCAGACTGATGTTGACT639410.7096238763724471No Hit
TCCTGTCTGAGCGTCGCT531960.5903747474626404No Hit
TATTGCACTTGTCCCGGCCTGTAT505130.5605985340736213No Hit
TATTGCACTTGTCCCGGCCTGTAA476310.5286137979621217No Hit
TGAGGTAGTAGATTGTATAGTT475400.5276038704860126No Hit
TTCAAGTAATTCAGGATAGGTT386790.42926356976290453No Hit
TATTGCACTTGTCCCGGCCTGTA365760.40592425677106436No Hit
CCCATAAAGTAGAAAGCACTAC351990.3906421673798308No Hit
TGTAAACATCCTTGACTGGAAGCT341160.3786229206037191No Hit
ACCGGGTGCTGTAGGCTTT316470.3512217014991763No Hit
TTCTCACTACTGCACTTGACTAG309870.34389695277135196No Hit
ACCGGGTGCTGTAGGCTT309500.3434863229184285No Hit
CAACGGAATCCCAAAAGCAGCTGT298550.33133389889271997No Hit
ACCGGGTGCTGTAGGCTTA288140.31978077249019704No Hit
TTAATGCTAATCGTGATAGGGGTT275390.3056306897205364No Hit
TCGTACGACTCTTAGCGG272250.3021458850227533No Hit
AGCAGCATTGTACAGGGCTATGA266420.2956756903131752No Hit
ACTGGACTTGGAGTCAGAAGGC251820.2794724582788971No Hit
CATTGCACTTGTCTCGGTCTGA243680.2704386015145805No Hit
CGCCTGTCTGAGCGTCGCT239140.265400062238168No Hit
CCCATAAAGTAGAAAGCACT222320.24673305108634902No Hit
CAACACCAGTCGATGGGCTGT220240.24442464542667103No Hit
GACCCGCGGGCGCTCTCCAGTCCT217020.24085105589582342No Hit
CACCCGGCTGTGTGCACATGTGC204120.2265345015641668No Hit
TGAGGTAGTAGTTTGTACAGTT195880.21738966375851945No Hit
CTACGCCTGTCTGAGCGTCGCT195560.21703452442626128No Hit
TTCACAGTGGCTAAGTTCCG177080.1965252279883532No Hit
TGATGTAGTAGGTTGTATAGTT174050.19316250243603386No Hit
ACCCCCCACTGCTAAATTTGACTG168850.18739148828683894No Hit
CCCCCCACTGCTAAATTTGACTGG163550.18150949309631334No Hit
CTCCCACTGCTTCACTTGACTAG160140.17772503958693744No Hit
TGTAAACATCCCCGACTGGAAGCT160000.17756966612907452No Hit
TTCACAGTGGCTAAGTTCCGC157460.17475074767927545No Hit
TAGCAGCACGTAAATATTGGC154180.1711105695236294No Hit
CTTCTCACTACTGCACTTGACTAG153430.17027821171364937No Hit
CCCCCACTGCTAAATTTGACTGGCTT150560.1670930558274591No Hit
AACGGAATCCCAAAAGCAGCTG144670.16055627249308255No Hit
TGAGAACTGAATTCCATGGGTT143450.15920230378884834No Hit
AGCTCGGTCTGAGGCCCCTCAGT142770.1584476327077998No Hit
TTCAAGTAATCCAGGATAGGC141760.15732672419036No Hit
TACGCCTGTCTGAGCGTCGCT121660.13501953488289503No Hit
GGAATACCGGGTGCTGTAGGCTTT119870.133032974243076No Hit
TGAGGTAGTAGGTTGTGTGGTT119470.13258905007775332No Hit
TGTAAACATCCTACACTCTCAGCT119230.1323226955785597No Hit
TGAGGTAGTAGTTTGTGCTGTT117490.13039162545940602No Hit
GGAATACCGGGTGCTGTAGGCTT115590.12828298567412325No Hit
CAAAGAATTCTCCTTTTGGGCT114770.12737294113521175No Hit
TATTGCACTTGTCCCGGCCTGTT110780.12294479758611797No Hit
TCCACCACGTTCCCGTGG108720.12065858813470613No Hit
TCAGGCTCAGTCCCCTCCCGA106700.11841677109982655No Hit
TGAGGTAGTAGGTTGTATAGT106200.11786186589317321No Hit
TTCACAGTGGCTAAGTTCTGC104390.11585310904508805No Hit
CGGCCCCGCCGGGGTCGGC100370.11139167118359505No Hit
GCCTGTCTGAGCGTCGCG96250.10681925228077137No Hit
TTCACAGTGGCTAAGTTCTG96190.10675266365597297No Hit
CAACGGAATCCCAAAAGCAGCT92520.10267965943913733No Hit
TCAGGCTCAGTCCCCTCCCGAT90780.10074858931998364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)