FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9034895
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG171041018.931155259690343No Hit
CAACGGAATCCCAAAAGCAGCTG6917727.65666894856No Hit
CCCCCACTGCTAAATTTGACTG5146485.696225578714528No Hit
TTCAAGTAATCCAGGATAGGCT2634282.9156730653759673No Hit
TAGCAGCACGTAAATATTGGCG2317022.564523439398023No Hit
AAGCTGCCAGTTGAAGAACTGT2090722.314050135613087No Hit
AACATTCAACGCTGTCGGTGAGT1761591.9497625594984778No Hit
AACATTCAACGCTGTCGGTGAG1603761.7750732022895672No Hit
GCCTGTCTGAGCGTCGCT1518991.6812480941947858No Hit
TAGCTTATCAGACTGATGTTGACT1091431.208016252540843No Hit
TGAGGTAGTAGGTTGTATAGTT1018091.1268420939036923No Hit
TAGCTTATCAGACTGATGTTGAC1005681.1131064611154862No Hit
TATTGCACTTGTCCCGGCCTGTAT951001.052585558548273No Hit
TATTGCACTTGTCCCGGCCTGTAA868430.9611954538486612No Hit
TAGCTTATCAGACTGATGTTGA848960.9396456738014111No Hit
TATTGCACTTGTCCCGGCCTGT700160.7749508987099463No Hit
CATAAAGTAGAAAGCACTACT607210.6720720052640347No Hit
TGTAAACATCCTTGACTGGAAGCT592770.656089528433922No Hit
TGAGGTAGTAGATTGTATAGTT550230.6090054173291444No Hit
TATTGCACTTGTCCCGGCCTGTA531970.5887948891492375No Hit
TCCTGTCTGAGCGTCGCT501720.5553135924656567No Hit
TCGTACGACTCTTAGCGG418180.46284987263272015No Hit
CAACGGAATCCCAAAAGCAGCTGT387350.4287266205085947No Hit
AGCAGCATTGTACAGGGCTATGA381940.4227387257959279No Hit
TTCAAGTAATTCAGGATAGGTT376900.4171603543815396No Hit
ACTGGACTTGGAGTCAGAAGGC358960.39730400851365727No Hit
CCCATAAAGTAGAAAGCACTAC352330.3899657937363965No Hit
TTCTCACTACTGCACTTGACTAG339460.3757210238746549No Hit
TGTAAACATCCCCGACTGGAAGCT331270.36665617032627384No Hit
CCCCCACTGCTAAATTTGACTGGCTT288560.31938389986823307No Hit
ACCCCCCACTGCTAAATTTGACTG273230.30241635348280194No Hit
CACCCGGCTGTGTGCACATGTGC269510.2982989841055153No Hit
ACCGGGTGCTGTAGGCTT257740.2852717159413585No Hit
CTTCTCACTACTGCACTTGACTAG238660.2641535955868884No Hit
ACCGGGTGCTGTAGGCTTA236420.2616743194027158No Hit
CATTGCACTTGTCTCGGTCTGA233350.25827638284672927No Hit
CGCCTGTCTGAGCGTCGCT231470.25619556176358443No Hit
CCCCCCACTGCTAAATTTGACTGG221510.245171637301817No Hit
ACCGGGTGCTGTAGGCTTT219880.2433675211499414No Hit
CTCCCACTGCTTCACTTGACTAG208970.2312921179493508No Hit
TGAGGTAGTAGTTTGTACAGTT208420.23068336710055842No Hit
CAACACCAGTCGATGGGCTGT197990.2191392373680048No Hit
TAGCAGCACGTAAATATTGGC186560.20648828791037416No Hit
CCCATAAAGTAGAAAGCACT186220.20611196920384797No Hit
GACCCGCGGGCGCTCTCCAGTCCT176310.19514338572833442No Hit
AACGGAATCCCAAAAGCAGCTG176240.19506590834757903No Hit
CAAAGAATTCTCCTTTTGGGCT168910.18695291976276426No Hit
CCCCCCACTGCTAAATTTGACTGGCT161770.17905022692571412No Hit
AGCTCGGTCTGAGGCCCCTCAGT154930.17147958000618713No Hit
CAACGGAATCCCAAAAGCAGCT154310.17079335177663935No Hit
TAGCTTATCAGACTGATGTTGACA145720.1612857703382275No Hit
TATTGCACTTGTCCCGGCCTGTT143450.1587732895623026No Hit
TGATGTAGTAGGTTGTATAGTT143400.15871794857604876No Hit
TTCAAGTAATCCAGGATAGGC139620.1545341700152575No Hit
TGTAAACATCCTACACTCTCAGCT138950.1537926007994559No Hit
TTCACAGTGGCTAAGTTCCG125260.1386402387631511No Hit
CCCCCCACTGCTAAATTTGACTGA118960.1316672744951657No Hit
TGAGGTAGTAGTTTGTGCTGTT117120.12963072620102392No Hit
AACATTCAACGCTGTCGGTGAGTT115780.12814758776942067No Hit
TTCAAGTAATCCAGGATAGGCTT115060.1273506775673652No Hit
GGAATACCGGGTGCTGTAGGCTTT114650.1268968814800836No Hit
TGAGGTAGTAGGTTGTGTGGTT112730.12477178760793568No Hit
AACCGGGCGGAAACACCA110830.12266883013028929No Hit
TATTGCACTTGTCCCGGCCTGTAG108970.12061014544164597No Hit
CACTAGATTGTGAGCTCCTGGA108080.1196250758863274No Hit
ACTGGACTTGGAGTCAGAAGGCT105810.1171125951104025No Hit
TGTAAACATCCCCGACTGGAAGC104700.11588402521556698No Hit
CTCCCACTGCTTCACTTGACTAGCCT102960.11395815889393292No Hit
CCCCCACAACCGCGCTTGACTAG102940.11393602249943136No Hit
CCCCCCACTGCTAAATTTGACTGT99020.10959728917712935No Hit
TGAGGTAGTAGGTTGTATAGT99010.10958622097987858No Hit
ATCCCGGACGAGCCCCCA98770.10932058424586008No Hit
CTACGCCTGTCTGAGCGTCGCT97940.10840192387404612No Hit
CACTAGATTGTGAGCTCCTGGAA96500.10680810346993518No Hit
GGAATACCGGGTGCTGTAGGCTT96480.10678596707543364No Hit
TTCACAGTGGCTAAGTTCTGC92520.10240296096412853No Hit
TTCACAGTGGCTAAGTTCCGC92290.1021483924273608No Hit
CAAAGAATTCTCCTTTTGGGCTT90460.1001229123304698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)