FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6712307
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG90344213.459485688005628No Hit
CAACGGAATCCCAAAAGCAGCTG4915957.323785994889685No Hit
CCCCCACTGCTAAATTTGACTG3236244.821352777815437No Hit
TAGCTTATCAGACTGATGTTGACT2017003.0049281118995306No Hit
TAGCTTATCAGACTGATGTTGAC1994802.971854535258891No Hit
TAGCTTATCAGACTGATGTTGA1933812.8809915875421073No Hit
TTCAAGTAATCCAGGATAGGCT1900922.8319920408884753No Hit
TAGCAGCACGTAAATATTGGCG1860612.7719381726729724No Hit
AACATTCAACGCTGTCGGTGAGT1614372.4050896360967995No Hit
AAGCTGCCAGTTGAAGAACTGT1540242.2946507065305566No Hit
AACATTCAACGCTGTCGGTGAG1282091.9100586430269055No Hit
TATTGCACTTGTCCCGGCCTGTAA1108771.6518463771099863No Hit
TGAGGTAGTAGGTTGTATAGTT920741.3717191421667692No Hit
TATTGCACTTGTCCCGGCCTGTAT910791.3568956247084647No Hit
TGAGGTAGTAGATTGTATAGTT784151.1682272577818624No Hit
CATAAAGTAGAAAGCACTACT744901.1097525783609123No Hit
TGTAAACATCCTTGACTGGAAGCT598030.8909455422703402No Hit
TTCAAGTAATTCAGGATAGGTT571360.8512125562790857No Hit
GCCTGTCTGAGCGTCGCT520760.775828638350421No Hit
TATTGCACTTGTCCCGGCCTGTA393970.5869368013113823No Hit
TATTGCACTTGTCCCGGCCTGT389050.5796069816234567No Hit
AGCAGCATTGTACAGGGCTATGA341580.5088861400409725No Hit
TGTAAACATCCCCGACTGGAAGCT318620.47468031483065365No Hit
CATTGCACTTGTCTCGGTCTGA299040.44551001615390956No Hit
TTCTCACTACTGCACTTGACTAG289990.43202731937022554No Hit
ACTGGACTTGGAGTCAGAAGGC257350.3834002229039882No Hit
TCCTGTCTGAGCGTCGCT253900.37826041031794283No Hit
CTTCTCACTACTGCACTTGACTAG248820.3706922225100848No Hit
CCCCCACTGCTAAATTTGACTGGCTT245510.3657609820289805No Hit
TAGCTTATCAGACTGATGTTGACA244860.3647926115417546No Hit
TGAGGTAGTAGTTTGTACAGTT241490.35977198301567553No Hit
CAACGGAATCCCAAAAGCAGCTGT233590.34800255709400657No Hit
TGATGTAGTAGGTTGTATAGTT200080.2980793339756361No Hit
CACCCGGCTGTGTGCACATGTGC189470.2822725480226098No Hit
CAACGGAATCCCAAAAGCAGCT188860.281363769565367No Hit
TTATAAAGCAATGAGACTGATT185630.276551713144229No Hit
CAACACCAGTCGATGGGCTGT184480.2748384422822138No Hit
TGATGTAGTAGATTGTATAGTT166820.2485285610446602No Hit
CAAAGAATTCTCCTTTTGGGCT159740.2379807717376455No Hit
TCAGTGCACTACAGAACTTTGT158580.23625260286813463No Hit
CTCCCACTGCTTCACTTGACTAG152120.22662849002585847No Hit
CTCCCACTGCTTCACTTGACTAGCCT148790.22166745352976258No Hit
TAGCAGCACGTAAATATTGGC140630.20951067941320325No Hit
TGTAAACATCCTACACTCTCAGCT136950.2040282126547549No Hit
ACCCCCCACTGCTAAATTTGACTG136380.20317902622749526No Hit
CAAAGAATTCTCCTTTTGGGCTT131470.19586410454706554No Hit
TGAGGTAGTAGTTTGTGCTGTT126760.18884714301655156No Hit
TTCAAGTAATCCAGGATAGGCTT123880.1845565168577659No Hit
CCCATAAAGTAGAAAGCACTAC123810.1844522308052954No Hit
CACTAGATTGTGAGCTCCTGGA122200.18205365159847425No Hit
CACTAGATTGTGAGCTCCTGGAA121680.18127895520869353No Hit
CGCCCCACTGCTAAATTTGACTG121150.180489360811417No Hit
CCCCCCACTGCTAAATTTGACTGG120550.17959548036167No Hit
CCCCCCACTGCTAAATTTGACTGGCT119190.17756935134224344No Hit
TATTGCACTTGTCCCGGCCTGTAG116710.17387464548328913No Hit
CCCATAAAGTAGAAAGCACT114510.17059708383421676No Hit
TCAGTGCACTACAGAACTTTGTC110860.16515931109825577No Hit
TATTGCACTTGTCCCGGCCTGTAAT107750.16052603076706712No Hit
AACGGAATCCCAAAAGCAGCTG106910.1592745981374213No Hit
AACATTCAACGCTGTCGGTGAGTT106760.15905112802498456No Hit
TTCACAGTGGCTAAGTTCCGC102380.1525258007418314No Hit
TTAGGTAGTAGGTTGTATAGTT97790.14568761530126675No Hit
CTCCCCACTGCTAAATTTGACTG89160.1328306348324056No Hit
TTAGGTAGTAGATTGTATAGTT85180.12690122784908378No Hit
TGTCAGTTTGTCAAATACCCCA85080.12675224777412594No Hit
TTCACAGTGGCTAAGTTCTGC84960.12657347168417654No Hit
TGAGGTAGTAAGTTGTATTGTT78970.11764956519420224No Hit
TGTCAGTTTGTCAAATACCCCAA78250.11657690865450582No Hit
TGAGAACTGAATTCCATAGGCTGT78190.11648752060953113No Hit
TGTAAACATCCCCGACTGGAAGC78140.1164130305720522No Hit
TATTGCACTTGTCCCGGCCTGTT77420.1153403740323558No Hit
TCAGTGCATCACAGAACTTTGTTT77150.11493812782996964No Hit
TTTAAACATCCTTGACTGGAAGCT77120.11489343380748228No Hit
TATTGCACTTGTCCCGGCCTGTAAA76910.11458057565007083No Hit
TAGCTTATCAGACTGATGTTG76260.1136122051628449No Hit
ACCGGGTGCTGTAGGCTTA76040.11328444899793767No Hit
ACTGGACTTGGAGTCAGAAGGCT75340.11224158847323283No Hit
TTCACAGTGGCTAAGTTCTG75330.11222669046573704No Hit
TGCAAGTAATCCAGGATAGGCT72370.10781688024698512No Hit
TAGCTTATCAGACTGATGTTGACC71570.10662503964732244No Hit
ACTGGACTTGGAGTCAGAAGGCA71240.10613340539996159No Hit
AGCAGCATTGTACAGGGCTATGT70440.10494156480029891No Hit
TTCAAGTAATCCAGGATAGGC70290.10471809468786217No Hit
CAGTGCAATAGTATTGTCAAAGC68970.10275155769841873No Hit
ACCGGGTGCTGTAGGCTT68870.1026025776234609No Hit
CCCTCCACTGCTAAATTTGACTG68030.10135114499381509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)