FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10444879
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG9283688.888259978885346No Hit
CAACGGAATCCCAAAAGCAGCTG6482616.2064960254685575No Hit
AACATTCAACGCTGTCGGTGAGT3826203.6632305649495795No Hit
AAGCTGCCAGTTGAAGAACTGT3541083.390254688445888No Hit
TAGCAGCACGTAAATATTGGCG3503343.3541221492369613No Hit
CCCCCACTGCTAAATTTGACTG2873592.751195107190806No Hit
TTCAAGTAATCCAGGATAGGCT2843012.7219176019176476No Hit
TAGCTTATCAGACTGATGTTGAC2051901.9645033705033825No Hit
AACATTCAACGCTGTCGGTGAG1695481.623264376734283No Hit
TAGCTTATCAGACTGATGTTGACT1529881.4647177817952701No Hit
TAGCTTATCAGACTGATGTTGA1426821.3660474190270657No Hit
TATTGCACTTGTCCCGGCCTGTAA1238661.1859017227485356No Hit
TATTGCACTTGTCCCGGCCTGTAT1062621.0173597989981502No Hit
CATAAAGTAGAAAGCACTACT963470.9224328974993392No Hit
TATTGCACTTGTCCCGGCCTGT918170.8790623615649353No Hit
TGTAAACATCCTTGACTGGAAGCT871240.8341312522624723No Hit
TGAGGTAGTAGGTTGTATAGTT823670.788587402496477No Hit
AGCAGCATTGTACAGGGCTATGA719480.6888351698473482No Hit
CGCCTGTCTGAGCGTCGCT665400.637058600678859No Hit
TATTGCACTTGTCCCGGCCTGTA620710.5942720829987594No Hit
ACTGGACTTGGAGTCAGAAGGC596710.571294315616294No Hit
TGTAAACATCCCCGACTGGAAGCT543160.5200251721441675No Hit
TGAGGTAGTAGATTGTATAGTT470740.45068975906757747No Hit
CCCATAAAGTAGAAAGCACTAC452190.4329298596948802No Hit
TTCTCACTACTGCACTTGACTAG430880.41252751707319923No Hit
TTCAAGTAATTCAGGATAGGTT424800.4067064826696412No Hit
CAACGGAATCCCAAAAGCAGCTGT407580.3902199345727222No Hit
CTTCTCACTACTGCACTTGACTAG399810.38278088238264896No Hit
TCGTACGACTCTTAGCGG378470.36234981755173995No Hit
TGTAAACATCCTACACTCTCAGCT375170.3591903745366509No Hit
CATTGCACTTGTCTCGGTCTGA367090.3514545261845542No Hit
ACCGGGTGCTGTAGGCTT356680.3414879195824097No Hit
CTCCCACTGCTTCACTTGACTAG347980.33315847890626593No Hit
AACATTCAACGCTGTCGGTGAGTT334550.32030050324182785No Hit
CCCATAAAGTAGAAAGCACT331060.3169591529016277No Hit
CCCCCACTGCTAAATTTGACTGGCTT312650.29933329050532803No Hit
CCCCCCACTGCTAAATTTGACTGGCT308710.29556110702670657No Hit
CTACGCCTGTCTGAGCGTCGCT281140.2691653967460992No Hit
ACCGGGTGCTGTAGGCTTA251970.24123783530666082No Hit
ACCGGGTGCTGTAGGCTTT246230.23574231927435446No Hit
CCCCCCACTGCTAAATTTGACTGG245200.23475619009085696No Hit
CAACACCAGTCGATGGGCTGT233160.22322901012065335No Hit
TGTCAGTTTGTCAAATACCCCAA230480.22066315942961137No Hit
TACGCCTGTCTGAGCGTCGCT226000.2163739761848845No Hit
TAGCTTATCAGACTGATGTTGACA218330.20903066469223816No Hit
CACCCGGCTGTGTGCACATGTGC211800.20277879715025898No Hit
TGAGGTAGTAGTTTGTACAGTT208420.1995427615772284No Hit
CTCCCACTGCTTCACTTGACTAGCCT200720.19217072787535402No Hit
GCGTTCGCGCTTTCCCCTG190660.18253921371420387No Hit
TGTAAACATCCTACACTCAGCT184040.17620117954454043No Hit
CAACGGAATCCCAAAAGCAGCT182540.17476506908313633No Hit
TTCAAGTAATCCAGGATAGGCTT178830.17121308920859685No Hit
ACGCCTGTCTGAGCGTCGCT177400.16984399723539162No Hit
GGAATACCGGGTGCTGTAGGCTTT174520.16708666514949574No Hit
GACCCGCGGGCGCTCTCCAGTCCT163940.15695729936172548No Hit
TGTAAACATCCCCGACTGGAAGC163420.1564594477351054No Hit
TGATGTAGTAGGTTGTATAGTT160460.15362552309126798No Hit
TGTCAGTTTGTCAAATACCCCA158330.15158624623607414No Hit
TGAGGTAGTAGTTTGTGCTGTT157110.15041820972746547No Hit
CACTAGATTGTGAGCTCCTGGA156270.14961398786907917No Hit
TTAATGCTAATCGTGATAGGGGTT154190.14762258136259884No Hit
CAAAGAATTCTCCTTTTGGGCT153210.14668432252781483No Hit
TAGCAGCACGTAAATATTGGC152300.14581308218122968No Hit
CAAAGAATTCTCCTTTTGGGCTT151570.145114175090013No Hit
AGCTCGGTCTGAGGCCCCTCAGT148920.1425770466081991No Hit
TATTGCACTTGTCCCGGCCTGTAG145880.13966652940642013No Hit
ACCACAGGGTAGAACCACGGAC145400.1392069740587708No Hit
ACTGGACTTGGAGTCAGAAGGCA145400.1392069740587708No Hit
AACGGAATCCCAAAAGCAGCTG144730.1385655113860103No Hit
GGAATACCGGGTGCTGTAGGCTT142940.13685175290206808No Hit
TTATAAAGCAATGAGACTGATT142750.13666984557695688No Hit
TATTGCACTTGTCCCGGCCTGTT141360.13533904988272244No Hit
ACTTGACTTGGAGTCAGAAGGC137680.13181579221741105No Hit
CGCGACCTCAGATCAGACGTGG136590.13077221861545738No Hit
TAGCAGCACGTAAATATTGGCGT136470.13065732977854508No Hit
AGCAGCATTGTACAGGGCTATGT136320.13051371873240464No Hit
CACTAGATTGTGAGCTCCTGGAA134850.12910633048022863No Hit
GGGCTACGCCTGTCTGAGCGTCGCT132350.12671281304455514No Hit
ACCCCCCACTGCTAAATTTGACTG131090.1255064802569757No Hit
AACATTCAACGCTGTCGGTGA129170.12366825886637844No Hit
TTCAAGTAATCCAGGATAGGC127460.12203109294037777No Hit
ACTGGACTTGGAGTCAGAAGG126210.120834334222541No Hit
TTCACAGTGGCTAAGTTCCG124300.11900568690168646No Hit
AGCAGCATTGTACAGGGCTATG121580.11640153993167371No Hit
CCCCCACTGCTAAATTTGACTGGCT119260.1141803557513687No Hit
ATCCCGGACGAGCCCCCA115780.11084857948091117No Hit
GCCTGTCTGAGCGTCGCT114060.1092018394851678No Hit
CAAAGTGCTGTTCGTGCAGGTAG108690.10406056403334113No Hit
TCAGTGCACTACAGAACTTTGT107890.1032946384539256No Hit
ACTGGACTTGGAGTCAGAAGGCT107320.10274891647859205No Hit
AACATTCAACGCTGTCGGTGAGA106490.10195426868994843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)