FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7916223
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG80040510.110945586045265No Hit
CAACGGAATCCCAAAAGCAGCTG5898587.451255478780727No Hit
CCCCCACTGCTAAATTTGACTG2696783.4066498631986493No Hit
GCCTGTCTGAGCGTCGCT2421463.058857740616958No Hit
TAGCAGCACGTAAATATTGGCG2221902.8067678234936033No Hit
AACATTCAACGCTGTCGGTGAGT1977892.4985273911561108No Hit
TTCAAGTAATCCAGGATAGGCT1917102.4217357191680935No Hit
AAGCTGCCAGTTGAAGAACTGT1622252.0492727402954665No Hit
AACATTCAACGCTGTCGGTGAG1513701.9121492661336095No Hit
TATTGCACTTGTCCCGGCCTGTAT1284391.6224782955204773No Hit
TATTGCACTTGTCCCGGCCTGTAA1185081.4970270544425037No Hit
TAGCTTATCAGACTGATGTTGACT1121921.4172415304621913No Hit
TAGCTTATCAGACTGATGTTGAC1114991.4084873556492787No Hit
TAGCTTATCAGACTGATGTTGA1082981.3680514053229678No Hit
TCCTGTCTGAGCGTCGCT887781.1214691652824837No Hit
TATTGCACTTGTCCCGGCCTGT839201.0601015155838838No Hit
TGAGGTAGTAGGTTGTATAGTT791741.0001486820166638No Hit
CATAAAGTAGAAAGCACTACT688020.869126602421382No Hit
TATTGCACTTGTCCCGGCCTGTA669280.8454536967945446No Hit
TGAGGTAGTAGATTGTATAGTT621830.7855134955142118No Hit
TGTAAACATCCTTGACTGGAAGCT513990.6492869137213543No Hit
TTCAAGTAATTCAGGATAGGTT484190.611642698797141No Hit
AGCAGCATTGTACAGGGCTATGA442760.5593071342229747No Hit
TGTAAACATCCCCGACTGGAAGCT367950.46480499601893477No Hit
ACCGGGTGCTGTAGGCTTA324760.4102461489526002No Hit
CATTGCACTTGTCTCGGTCTGA313070.3954790055813233No Hit
CTCCCACTGCTTCACTTGACTAG293720.3710355304543594No Hit
ACTGGACTTGGAGTCAGAAGGC288670.3646562255762628No Hit
CAACGGAATCCCAAAAGCAGCTGT283700.35837797899326485No Hit
CCCATAAAGTAGAAAGCACT282060.35630628394374436No Hit
TTCTCACTACTGCACTTGACTAG276340.3490806158441974No Hit
ACCGGGTGCTGTAGGCTT261880.33081432900513286No Hit
CACCCGGCTGTGTGCACATGTGC236370.2985893651555799No Hit
CCCATAAAGTAGAAAGCACTAC224840.2840243383745001No Hit
CTTCTCACTACTGCACTTGACTAG224360.2834179886039087No Hit
TCGTACGACTCTTAGCGG221200.2794261859475156No Hit
CCCCCACTGCTAAATTTGACTGGCTT220460.27849139671785395No Hit
TGATGTAGTAGGTTGTATAGTT215250.2719099752495603No Hit
CAACACCAGTCGATGGGCTGT210790.2662759752978156No Hit
ATCCCGGACGAGCCCCCA210160.2654801412239145No Hit
TGAGGTAGTAGTTTGTACAGTT206740.2611598991084511No Hit
ACCGGGTGCTGTAGGCTTT201790.25490691709922775No Hit
TGATGTAGTAGATTGTATAGTT171180.21623948693714162No Hit
CAACGGAATCCCAAAAGCAGCT162350.20508517761563816No Hit
AGAATTACTCTGAGACCT162120.2047946350172298No Hit
CCCCCCACTGCTAAATTTGACTGGCT161710.2042767112548497No Hit
TATTGCACTTGTCCCGGCCTGTAG160750.203064011713667No Hit
TATTGCACTTGTCCCGGCCTGTT158300.1999691014262736No Hit
GGAATACCGGGTGCTGTAGGCTTT153470.19386770685969812No Hit
ACCCCCCACTGCTAAATTTGACTG149340.188650572375235No Hit
TGTAAACATCCTACACTCTCAGCT147290.18606095356333444No Hit
CACTAGATTGTGAGCTCCTGGAA145770.1841408459564618No Hit
CACTAGATTGTGAGCTCCTGGA144660.18273866211196932No Hit
CCCCCCACTGCTAAATTTGACTGG144260.18223337063647652No Hit
CGCCTGTCTGAGCGTCGCT141880.1792268863572944No Hit
TAGCTTATCAGACTGATGTTGACA139780.1765741061109572No Hit
AACATTCAACGCTGTCGGTGAGTT139060.17566458145507016No Hit
GACCCGCGGGCGCTCTCCAGTCCT137920.1742245007499157No Hit
TAGCAGCACGTAAATATTGGC134770.17024533038040995No Hit
TCAGTGCACTACAGAACTTTGT131290.16584929454362263No Hit
AACGGAATCCCAAAAGCAGCTG130710.16511662190415807No Hit
CAAAGAATTCTCCTTTTGGGCT129230.16324704344483473No Hit
TGAGGTAGTAGTTTGTGCTGTT127150.1606195277722722No Hit
GGAATACCGGGTGCTGTAGGCTT126750.1601142362967794No Hit
CTCCCACTGCTTCACTTGACTAGCCT123080.15547818700913302No Hit
AGCTCGGTCTGAGGCCCCTCAGT121980.15408863545152782No Hit
TTATAAAGCAATGAGACTGATT120240.15189061753313415No Hit
TGTAAACATCCCCGACTGGAAGC115570.14599133955675578No Hit
AACCGGGCGGAAACACCA106470.1344959584892947No Hit
TTCAAGTAATCCAGGATAGGCTT106180.13412962216956245No Hit
TATTGCACTTGTCCCGGCCTGTTT101630.1283819316358319No Hit
TGTCAGTTTGTCAAATACCCCAA100670.1271692320946492No Hit
ATCCCACCGCTGCCACCA98050.12385957293017137No Hit
TGTCAGTTTGTCAAATACCCCA97840.12359429490553765No Hit
CAAAGAATTCTCCTTTTGGGCTT96580.12200262675773535No Hit
ACTGGACTTGGAGTCAGAAGGCA95580.12073939806900337No Hit
TTCACAGTGGCTAAGTTCCG92670.11706340258479328No Hit
AGCAGCATTGTACAGGGCTATGT92190.11645705281420192No Hit
TTCACAGTGGCTAAGTTCCGC90400.11419587346137167No Hit
TATTGCACTTGTCCCGGCCTGA90150.11388006628918868No Hit
TTCAAGTAATCCAGGATAGGC90040.11374111113342815No Hit
CTACGCCTGTCTGAGCGTCGCT89230.11271789589555525No Hit
ACTTGACTTGGAGTCAGAAGGC86670.10948403045240136No Hit
CCCGCCACTGCTAAATTTGACTG84170.10632595873057138No Hit
TATTGCACTTGTCCCGGCCTGTAAT82290.10395108879575525No Hit
ACCTGGTGCTGTAGGCTTA80990.10230889150040366No Hit
TTCACAGTGGCTAAGTTCTGC80960.10227099463974171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)