FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9262110
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG128547113.878813790810085No Hit
CAACGGAATCCCAAAAGCAGCTG7524128.123548521881082No Hit
CCCCCACTGCTAAATTTGACTG6458326.9728388023895205No Hit
AACATTCAACGCTGTCGGTGAGT3178543.4317666276906666No Hit
TTCAAGTAATCCAGGATAGGCT2573412.7784273777789292No Hit
TAGCAGCACGTAAATATTGGCG2447002.6419465974815672No Hit
AAGCTGCCAGTTGAAGAACTGT2378372.567849010646602No Hit
AACATTCAACGCTGTCGGTGAG2173002.346117677289516No Hit
TAGCTTATCAGACTGATGTTGAC1932542.0865008081311927No Hit
TAGCTTATCAGACTGATGTTGACT1637101.7675238147679093No Hit
TAGCTTATCAGACTGATGTTGA1575161.7006492041230348No Hit
TGAGGTAGTAGGTTGTATAGTT1420351.5335058642145256No Hit
GCCTGTCTGAGCGTCGCT1330761.4367784446524603No Hit
TATTGCACTTGTCCCGGCCTGTAA1300651.404269653459093No Hit
CATAAAGTAGAAAGCACTACT1131561.2217086603376552No Hit
TATTGCACTTGTCCCGGCCTGTAT1062151.1467689327809754No Hit
TGAGGTAGTAGATTGTATAGTT997521.0769900163137773No Hit
GGAATACCGGGTGCTGTAGGCTTT850280.9180197600762676No Hit
TATTGCACTTGTCCCGGCCTGT814650.8795512037753815No Hit
TGTAAACATCCTTGACTGGAAGCT717210.7743483936165734No Hit
TTCAAGTAATTCAGGATAGGTT664190.7171044178918196No Hit
TATTGCACTTGTCCCGGCCTGTA625750.675601995657577No Hit
GGAATACCGGGTGCTGTAGGCTT596270.6437733950471328No Hit
CATTGCACTTGTCTCGGTCTGA562350.6071510703284673No Hit
AGCAGCATTGTACAGGGCTATGA522140.5637376364564878No Hit
ACTGGACTTGGAGTCAGAAGGC446040.48157493270971735No Hit
TGTAAACATCCCCGACTGGAAGCT406770.4391763863741631No Hit
TGAGGTAGTAGTTTGTACAGTT392710.42399626003146146No Hit
TGTCAGTTTGTCAAATACCCCAA392030.42326208606894106No Hit
CAACGGAATCCCAAAAGCAGCTGT354520.38276375469520446No Hit
CACCCGGCTGTGTGCACATGTGC318910.3443167917461572No Hit
CCCATAAAGTAGAAAGCACT308520.3330990454658819No Hit
CAACGGAATCCCAAAAGCAGCT307450.3319438011425042No Hit
TTATAAAGCAATGAGACTGATT295710.3192685036131076No Hit
CCCATAAAGTAGAAAGCACTAC274860.2967574343211212No Hit
CAACACCAGTCGATGGGCTGT265790.2869648492622091No Hit
TTCTCACTACTGCACTTGACTAG257050.2775285545086379No Hit
TGTCAGTTTGTCAAATACCCCA250930.2709209888459541No Hit
TGAGGTAGTAGTTTGTGCTGTT231370.24980269074757264No Hit
AACGGAATCCCAAAAGCAGCTG209770.2264818707616299No Hit
AACATTCAACGCTGTCGGTGAGTT208160.22474360593860362No Hit
CACTAGATTGTGAGCTCCTGGA197140.21284566907540506No Hit
TGATGTAGTAGGTTGTATAGTT189880.20500728235790766No Hit
TAGCTTATCAGACTGATGTTGACA188460.20347415437735028No Hit
TGTAAACATCCTACACTCTCAGCT184810.19953336766676275No Hit
TAGCAGCACGTAAATATTGGC177210.19132789396800512No Hit
CTTCTCACTACTGCACTTGACTAG168820.1822694828716135No Hit
TTCAAGTAATCCAGGATAGGCTT154840.16717572993626723No Hit
TTCACAGTGGCTAAGTTCCGC151310.1633645033367127No Hit
CACTAGATTGTGAGCTCCTGGAA147940.15972602355186885No Hit
ACCCCCCACTGCTAAATTTGACTG145580.1571780080348862No Hit
TCGTACGACTCTTAGCGG143190.15459760249014534No Hit
CAAAGAATTCTCCTTTTGGGCT141250.15250304736177825No Hit
TGATGTAGTAGATTGTATAGTT135310.146089821865644No Hit
CCCCCACTGCTAAATTTGACTGGCTT134140.1448266107830721No Hit
TCAGTGCACTACAGAACTTTGT133490.14412482684830996No Hit
GACCCGCGGGCGCTCTCCAGTCCT132210.14274285233062445No Hit
GGAATACCGGGTGCTGTAGGCTTA131620.14210584845137877No Hit
CAAAGAATTCTCCTTTTGGGCTT128830.13909357586986118No Hit
TATTGCACTTGTCCCGGCCTGTT127260.13739849775051258No Hit
ACTGGACTTGGAGTCAGAAGGCT126290.13635122018632903No Hit
CCCCCCACTGCTAAATTTGACTGG123810.13367364455831338No Hit
CTCCCACTGCTTCACTTGACTAG121810.13151430937442982No Hit
CGCCTGTCTGAGCGTCGCT121800.13150351269851038No Hit
TGAGGTAGTAAGTTGTATTGTT116670.125964817951849No Hit
ACCGGGTGCTGTAGGCTT115430.12462603013784117No Hit
AGCAGCATTGTACAGGGCTATGT112030.12095516032523906No Hit
TGAGGTAGTAGGTTGTATAGT110980.11982150935370017No Hit
TTCACAGTGGCTAAGTTCTGC110630.11944362569652055No Hit
ACTGGACTTGGAGTCAGAAGGCA109860.11861228165072538No Hit
TTCAAGTAATCCAGGATAGGC109440.11815882126210982No Hit
TTCACAGTGGCTAAGTTCTG105150.11352704729267953No Hit
TGTAAACATCCCCGACTGGAAGC103700.11196152928436394No Hit
TATTGCACTTGTCCCGGCCTGTAAT103140.11135691543287653No Hit
CCCATAAAGTAGAAAGCACTA102390.11054716473892018No Hit
ACTGGACTTGGAGTCAGAAGG100430.10843101625871428No Hit
ACCACAGGGTAGAACCACGGAC99760.10770763897211327No Hit
ACCGGGTGCTGTAGGCTTT97720.105505117084552No Hit
AACATTCAACGCTGTCGGTGA97620.10539715032535782No Hit
ACCGGGTGCTGTAGGCTTA94930.1024928445030344No Hit
GGAATACCGGGTGCTGTAGGCT94260.10176946721643342No Hit
TGAGGTAGTAGATTGTATAGT94020.10151034699436737No Hit
TATTGCACTTGTCCCGGCCTGTAG93370.10080856305960521No Hit
AGCAGCATTGTACAGGGCTATG93270.10070059630041103No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)