FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6797155
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5251787.72643848786735No Hit
CAACGGAATCCCAAAAGCAGCTG3908835.750685397052149No Hit
AAGCTGCCAGTTGAAGAACTGT2571523.783229895448905No Hit
AACATTCAACGCTGTCGGTGAGT2519903.7072863573068435No Hit
TAGCAGCACGTAAATATTGGCG2224603.2728398866878865No Hit
CCCCCACTGCTAAATTTGACTG2147453.1593365165278704No Hit
TTCAAGTAATCCAGGATAGGCT1820812.6787825200396345No Hit
GCCTGTCTGAGCGTCGCT1805162.6557581811802145No Hit
AACATTCAACGCTGTCGGTGAG1111981.635949158140428No Hit
CGCCTGTCTGAGCGTCGCT866871.2753424042853223No Hit
TAGCTTATCAGACTGATGTTGAC761531.1203658001031314No Hit
TATTGCACTTGTCCCGGCCTGT741171.0904120915294708No Hit
TCCTGTCTGAGCGTCGCT718511.057074614305544No Hit
CATAAAGTAGAAAGCACTACT704801.0369044107424357No Hit
TAGCTTATCAGACTGATGTTGACT594260.8742775470031211No Hit
TATTGCACTTGTCCCGGCCTGTAA559880.8236975617004467No Hit
TAGCTTATCAGACTGATGTTGA555740.8176067781299676No Hit
TGAGGTAGTAGGTTGTATAGTT493880.7265981134754173No Hit
TATTGCACTTGTCCCGGCCTGTAT474520.698115608662742No Hit
TGTAAACATCCTTGACTGGAAGCT442680.6512724809129702No Hit
GGAATACCGGGTGCTGTAGGCTTT393940.5795660096025469No Hit
TATTGCACTTGTCCCGGCCTGTA360530.5304130919480282No Hit
TCGTACGACTCTTAGCGG337620.4967078137838552No Hit
GGAATACCGGGTGCTGTAGGCTT329280.48443797441723774No Hit
AGCAGCATTGTACAGGGCTATGA328740.48364352438630576No Hit
CCCATAAAGTAGAAAGCACTAC313240.46083986609103367No Hit
ACTGGACTTGGAGTCAGAAGGC279700.4114956919475869No Hit
TTCAAGTAATTCAGGATAGGTT265890.39117836800837996No Hit
CAACGGAATCCCAAAAGCAGCTGT263190.38720611785371967No Hit
TGAGGTAGTAGATTGTATAGTT261360.3845138149711166No Hit
AACCGGGCGGAAACACCA260920.3838664853162831No Hit
CATTGCACTTGTCTCGGTCTGA232440.34196660220342184No Hit
TGTAAACATCCCCGACTGGAAGCT228700.3364643001373369No Hit
ACGCCTGTCTGAGCGTCGCT223910.3294172341222173No Hit
ATCCCGGACGAGCCCCCA213260.3137489140677239No Hit
TTCTCACTACTGCACTTGACTAG211480.31113017137317006No Hit
AACATTCAACGCTGTCGGTGAGTT210830.31017388892852965No Hit
CCCATAAAGTAGAAAGCACT206520.303833000718683No Hit
CTACGCCTGTCTGAGCGTCGCT202880.2984778190286965No Hit
TACGCCTGTCTGAGCGTCGCT199630.29369640680549436No Hit
CAACACCAGTCGATGGGCTGT181790.2674501317095167No Hit
TGAATACCGGGTGCTGTAGGCTTT175280.25787259522550243No Hit
TGATGTAGTAGGTTGTATAGTT174510.25673976832954376No Hit
GCGTTCGCGCTTTCCCCTG168980.24860401153129508No Hit
ACCGGGTGCTGTAGGCTT161320.23733459072214774No Hit
TGTAAACATCCTACACTCTCAGCT153030.22513831154357963No Hit
TGAATACCGGGTGCTGTAGGCTT147580.21712025104620977No Hit
TGTCAGTTTGTCAAATACCCCAA136410.20068690503600406No Hit
CTCCCACTGCTTCACTTGACTAG128110.1884759138198261No Hit
TGAGGTAGTAGTTTGTACAGTT125770.1850332970191205No Hit
CTTCTCACTACTGCACTTGACTAG125600.18478319237975302No Hit
TTCACAGTGGCTAAGTTCCG120420.17716235689784918No Hit
CACCCGGCTGTGTGCACATGTGC110910.163171209130879No Hit
ACTTGACTTGGAGTCAGAAGGC109000.1603612099473971No Hit
ACCACAGGGTAGAACCACGGAC106790.1571098496356196No Hit
TTAATGCTAATCGTGATAGGGGTT106620.1568597449962521No Hit
TATTGCACTTGTCCCGGCCTGTT105720.15553566161136534No Hit
CCCCCCACTGCTAAATTTGACTGG104990.15446168286584608No Hit
AGCTCGGTCTGAGGCCCCTCAGT103060.15162225960714446No Hit
ACCGGGTGCTGTAGGCTTT101060.14867985208517387No Hit
GACCCGCGGGCGCTCTCCAGTCCT96690.14225069164966814No Hit
TGAGGTAGTAGTTTGTGCTGTT96660.14220655553683859No Hit
TTCAAGTAATCCAGGATAGGCTT94330.13877865077374282No Hit
TAGCAGCACGTAAATATTGGC93790.1379842007428108No Hit
CAACGGAATCCCAAAAGCAGCT93650.13777823221627283No Hit
CCCCCACTGCTAAATTTGACTGGCTT93000.1368219497716324No Hit
TGCAAGTAATCCAGGATAGGCT92690.13636587660572694No Hit
CCCCCCACTGCTAAATTTGACTGGCT91130.1340707987385899No Hit
TTATAAAGCAATGAGACTGATT90250.13277613942892286No Hit
TGATGTAGTAGATTGTATAGTT90040.1324671866391159No Hit
TTCAAGTAATCCAGGATAGGC89160.13117252732944887No Hit
AACGGAATCCCAAAAGCAGCTG88980.1309077106524715No Hit
TACCTGGTTGATCCTGCC87410.12859792074772458No Hit
CAAAGTGCTGTTCGTGCAGGT85300.12549368081204562No Hit
TGTAAACATCCCCGACTGGAAGC83080.12222760846265826No Hit
ACCGGGTGCTGTAGGCTTA82780.12178624733436268No Hit
ATCCCACCGCTGCCACCA82360.12116834175474886No Hit
TGTAAACATCCTACACTCAGCT81500.1199031065203015No Hit
CGCCCCACTGCTAAATTTGACTG79160.11646048971959591No Hit
TGTCAGTTTGTCAAATACCCCA78360.11528352671080769No Hit
TCGTTCGCGCTTTCCCCTG78180.11501871003383032No Hit
CCTCGACACAAGGGTTTGT77140.11348865812240562No Hit
TAGCTTATCAGACTGATGTTGACA76850.1130620090317199No Hit
AACATTCAACGCTGTCGGTGA75690.11135541266897694No Hit
TAGCAGCACGTAAATATTGGCGT75370.11088462746546164No Hit
CACTAGATTGTGAGCTCCTGGA75170.1105903867132646No Hit
AGCAGCATTGTACAGGGCTATG74670.10985478483277196No Hit
CTCCCCACTGCTAAATTTGACTG72960.1073390264014871No Hit
TAGTGGGTTATCAGAACT72120.10610321524225945No Hit
TGAGGTAGTAGGTTGTGTGGTT71860.10572070226440326No Hit
CCCGCCACTGCTAAATTTGACTG69670.10249876602784547No Hit
CAAAGAATTCTCCTTTTGGGCTT68780.10118939468056855No Hit
ACTGGACTTGGAGTCAGAAGG68390.1006156252137843No Hit
CAAAGAATTCTCCTTTTGGGCT68280.10045379280007591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)