FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10929103
Sequences flagged as poor quality0
Sequence length18-26
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8823288.07319685796721No Hit
CAACGGAATCCCAAAAGCAGCTG5272384.824165350074932No Hit
AAGCTGCCAGTTGAAGAACTGT4066223.7205432138392327No Hit
CCCCCACTGCTAAATTTGACTG3999383.6593854042733422No Hit
TTCAAGTAATCCAGGATAGGCT3837383.5111573200472175No Hit
TAGCTTATCAGACTGATGTTGAC3718253.402154778850561No Hit
TAGCAGCACGTAAATATTGGCG3679263.366479389937125No Hit
TAGCTTATCAGACTGATGTTGACT3336723.0530593407345505No Hit
AACATTCAACGCTGTCGGTGAGT3280203.0013442091267692No Hit
GCCTGTCTGAGCGTCGCT2284202.090015987588368No Hit
TAGCTTATCAGACTGATGTTGA2166671.9824774274704886No Hit
CATAAAGTAGAAAGCACTACT2082241.9052249759197986No Hit
TGTAAACATCCTTGACTGGAAGCT1780851.629456690087009No Hit
AACATTCAACGCTGTCGGTGAG1751451.6025560377644898No Hit
TGAGGTAGTAGGTTGTATAGTT1415931.295559205545048No Hit
TGAGGTAGTAGATTGTATAGTT1241301.1357748206783302No Hit
TATTGCACTTGTCCCGGCCTGTAA1156511.0581929733849156No Hit
TCCTGTCTGAGCGTCGCT1040750.9522739423354323No Hit
TTCAAGTAATTCAGGATAGGTT954040.8729353177474858No Hit
TATTGCACTTGTCCCGGCCTGTAT934160.8547453528436872No Hit
TATTGCACTTGTCCCGGCCTGT647240.5922169458920828No Hit
AGCAGCATTGTACAGGGCTATGA619370.5667162254761439No Hit
TGTAAACATCCCCGACTGGAAGCT558420.5109476962564997No Hit
TGAGGTAGTAGTTTGTACAGTT539010.4931877757945917No Hit
TGTCAGTTTGTCAAATACCCCAA485540.444263358118228No Hit
TATTGCACTTGTCCCGGCCTGTA483020.44195758791915496No Hit
ACTGGACTTGGAGTCAGAAGGC466530.42686943292601415No Hit
TTATAAAGCAATGAGACTGATT417940.38241015753991886No Hit
TTCTCACTACTGCACTTGACTAG398990.36507113163815913No Hit
TAGCTTATCAGACTGATGTTGACA386010.3531945851365844No Hit
CCCATAAAGTAGAAAGCACTAC357460.32707167276216537No Hit
TGTAAACATCCTACACTCTCAGCT349710.31998051441184144No Hit
CATTGCACTTGTCTCGGTCTGA340310.31137962557402926No Hit
TGTCAGTTTGTCAAATACCCCA334130.3057249986572549No Hit
CAACGGAATCCCAAAAGCAGCTGT328250.300344868192751No Hit
TGATGTAGTAGGTTGTATAGTT303660.277845308988304No Hit
CAAAGAATTCTCCTTTTGGGCT302290.27659177518960154No Hit
GGAATACCGGGTGCTGTAGGCTTT298550.27316971941796137No Hit
AACATTCAACGCTGTCGGTGAGTT293460.2685124296111035No Hit
AACCGGGCGGAAACACCA273400.25015776683594254No Hit
TGATGTAGTAGATTGTATAGTT267560.24481423589840812No Hit
CAAAGAATTCTCCTTTTGGGCTT267530.24478678625318107No Hit
CAACACCAGTCGATGGGCTGT266310.24367050068061394No Hit
CTTCTCACTACTGCACTTGACTAG245050.22421785209637057No Hit
TTCAAGTAATCCAGGATAGGCTT240010.21960631169822445No Hit
TGAGGTAGTAGTTTGTGCTGTT239620.2192494663102727No Hit
CGCCTGTCTGAGCGTCGCT233020.21321054436031942No Hit
TTTAAACATCCTTGACTGGAAGCT225750.20655858033362845No Hit
TAGCAGCACGTAAATATTGGC224630.2055337935784849No Hit
TTCACAGTGGCTAAGTTCCGC218870.20026346169488932No Hit
TGTAAACATCCTACACTCAGCT201440.1843152178179673No Hit
GGAATACCGGGTGCTGTAGGCTT197710.1809023119280695No Hit
CCCCCACTGCTAAATTTGACTGGCTT192600.1762267223577269No Hit
TTCACAGTGGCTAAGTTCCG189090.17301511386616084No Hit
TGAATACCGGGTGCTGTAGGCTTT170380.15589568512621757No Hit
TAGCAGCACGTAAATATTGGCGT161030.14734054569711713No Hit
TGTAAACATCCCCGACTGGAAGC156650.14333289749396635No Hit
TGTCAGTTTGTCAAATACCCC153780.14070688143391089No Hit
TTCACAGTGGCTAAGTTCTGC152700.13971869420573674No Hit
TTAGGTAGTAGGTTGTATAGTT149470.1367632824029566No Hit
TTCAAGTAATCCAGGATAGGC149000.13633323796106597No Hit
TGCAAGTAATCCAGGATAGGCT141560.12952572594475503No Hit
TGTAAACATCCTTGACTGGAAGC139090.12726570515439373No Hit
TGAGGTAGTAAGTTGTATTGTT137570.125874923129556No Hit
CCCATAAAGTAGAAAGCACT136410.12481353684744301No Hit
TTAGGTAGTAGATTGTATAGTT134830.12336785553215118No Hit
ATCACATTGCCAGGGATTTCC129390.11839031986431092No Hit
ACTGGACTTGGAGTCAGAAGGCA129120.11814327305726738No Hit
CAGTGCAATAGTATTGTCAAAGC129040.11807007400332856No Hit
AGCAGCATTGTACAGGGCTATGT126410.11566365510508958No Hit
TGTAGTGTTTCCTACTTTATGGA126000.11528850995365311No Hit
TTAATGCTAATCGTGATAGGGGTT125610.11493166456570131No Hit
TATTGCACTTGTCCCGGCCTGTAG124970.11434607213419071No Hit
TAGCAGCACATCATGGTTTACA123010.11255269531268942No Hit
CCCCCCACTGCTAAATTTGACTGG122020.11164685702019644No Hit
TATTGCACTTGTCCCGGCCTGTT121850.11149130903057643No Hit
CACCCGGCTGTGTGCACATGTGC119690.10951493457422809No Hit
TAGCAGCACATAATGGTTTGT119340.10919468871324572No Hit
CCCTGTCTGAGCGTCGCT118890.10878294403483982No Hit
TTCACAGTGGCTAAGTTCTG117500.1075111104726527No Hit
CGCCCCACTGCTAAATTTGACTG117330.1073555624830327No Hit
CAACGGAATCCCAAAAGCAGCT116580.10666932135235617No Hit
TGATGTAGTAGTTTGTACAGTT116340.10644972419053969No Hit
AACGGAATCCCAAAAGCAGCTG116090.10622097714698085No Hit
CATAAAGTAGAAAGCACTACTA115900.10604712939387614No Hit
ACTGGACTTGGAGTCAGAAGGCT115080.10529683909100317No Hit
TTCACAGTGGCTAAGTTCCGCT115040.10526023956403374No Hit
CTCCCACTGCTTCACTTGACTAG114790.10503149252047493No Hit
ACCCCCCACTGCTAAATTTGACTG114580.1048393450038855No Hit
CCCCCCACTGCTAAATTTGACTGGCT114230.10451909914290312No Hit
TAGCTTATCAGACTGATGTTGACC113010.103402813570336No Hit
TGAATACCGGGTGCTGTAGGCTT112570.10300021877367245No Hit
CAAAGTGCTGTTCGTGCAGGTAG112550.10298191901018776No Hit
ACTGGACTTGGAGTCAGAAGG112500.10293616960147599No Hit
TCAGTGCATCACAGAACTTTGTTT111930.10241462634216184No Hit
ATCACATTGCCAGGGATTTCCA111920.10240547646041948No Hit
TCGTACGACTCTTAGCGG111850.10234142728822301No Hit
CTACGCCTGTCTGAGCGTCGCT111410.10193883249155947No Hit
TGAGGTAGTAGGTTGTATAGT109480.10017290531528526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)